دورية أكاديمية
أعرض في EDS

Bioprospection of the trichomonacidal activity of lipid extracts derived from marine macroalgae Gigartina skottsbergii.

التفاصيل البيبلوغرافية
العنوان: Bioprospection of the trichomonacidal activity of lipid extracts derived from marine macroalgae Gigartina skottsbergii.
المؤلفون: Barbosa TN; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Silva MTO; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Sena-Lopes Â; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Kremer FS; Laboratório de Lipidômica e Bio-orgânica, Centro de Ciências Químicas, Farmacêuticas e de Alimentos, UFPel, Pelotas, Rio Grande do Sul, Brasil., Sousa FSS; Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Seixas FK; Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Collares TV; Laboratório de Bioinformática e Proteômica, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., de Pereira CMP; Laboratório de Biotecnologia do Câncer, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil., Borsuk S; Laboratório de Biotecnologia Infecto-Parasitária, Centro de Desenvolvimento Tecnológico, Biotecnologia, UFPel, Pelotas, Rio Grande do Sul, Brasil.
المصدر: PloS one [PLoS One] 2023 May 08; Vol. 18 (5), pp. e0285426. Date of Electronic Publication: 2023 May 08 (Print Publication: 2023).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Seaweed* , Trichomonas Infections* , Trichomonas vaginalis* , Anti-Infective Agents*/pharmacology , Rhodophyta*, Female ; Humans ; Antiparasitic Agents/pharmacology ; Lipids/pharmacology
مستخلص: Algal extracts are sources of bioactive substances with applications in the development of novel alternative drugs against several diseases, including trichomoniasis sexually transmitted infection caused by Trichomonas vaginalis. Factors such as clinical failures and resistant strains limit the success of the existing drugs available for treating this disease. Therefore, searching for viable alternatives to these drugs is essential for the treatment of this disease. The present study was conducted for, in vitro and in silico characterization of extracts obtained from marine macroalgae Gigartina skottsbergii at stages gametophidic, cystocarpic, and tetrasporophidic. In addition, antiparasitic activity of these extracts against the ATCC 30236 isolate of T. vaginalis, their cytotoxicity, and gene expression of trophozoites after treatment were evaluated. The minimum inhibitory concentration and 50% inhibition concentration were determined for each extract. Results: In vitro analysis of the extracts' anti-T. vaginalis activity revealed an inhibitory effect of 100%, 89.61%, and 86.95% for Gigartina skottsbergii at stages gametophidic, cystocarpic, and tetrasporophidic, respectively, at 100 μg/mL. In silico analysis revealed the interactions between constituents of the extracts and enzymes from T. vaginalis, with significant free energy values obtained for the binding. None of the extract concentrations exhibited cytotoxic effects on VERO cell line compared to control, while cytotoxicity on HMVII vaginal epithelial cells line was observed at 100 μg/mL (30% inhibition). Gene expression analysis revealed differences in the expression profile of T. vaginalis enzymes between the extract-treated and control groups. According to these results, Gigartina skottsbergii extracts exhibited satisfactory antiparasitic activity.
Competing Interests: The authors declare that they have no competing interests.
(Copyright: © 2023 Barbosa et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
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المشرفين على المادة: 0 (Antiparasitic Agents)
0 (Anti-Infective Agents)
0 (Lipids)
SCR Organism: Gigartina
تواريخ الأحداث: Date Created: 20230508 Date Completed: 20230510 Latest Revision: 20230613
رمز التحديث: 20230613
مُعرف محوري في PubMed: PMC10166524
DOI: 10.1371/journal.pone.0285426
PMID: 37155662
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0285426