دورية أكاديمية
Anopheles Egg Collection, Disinfection, and Hatching.
| العنوان: | Anopheles Egg Collection, Disinfection, and Hatching. |
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| المؤلفون: | Leite LN; Entomology Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30333, USA., Bascuñán P; CDC Foundation, Atlanta, Georgia 30308, USA., Dotson EM; Entomology Branch, Division of Parasitic Diseases and Malaria, Centers for Disease Control and Prevention (CDC), Atlanta, Georgia 30333, USA., Benedict MQ; CDC Foundation, Atlanta, Georgia 30308, USA mbenedict@cdc.gov. |
| المصدر: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2024 Mar 01; Vol. 2024 (3), pp. 108186. Date of Electronic Publication: 2024 Mar 01. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Cold Spring Harbor Laboratory Press Country of Publication: United States NLM ID: 101524530 Publication Model: Electronic Cited Medium: Internet ISSN: 1559-6095 (Electronic) Linking ISSN: 15596095 NLM ISO Abbreviation: Cold Spring Harb Protoc Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press |
| مواضيع طبية MeSH: | Anopheles*, Animals ; Female ; Disinfection ; Oviposition ; Larva ; Time Factors |
| مستخلص: | Gravid (i.e., with fully developed eggs), mated Anopheles females typically lay their eggs directly on water ∼48-72 h after a blood meal. Unlike some other mosquito species, Anopheles eggs cannot be desiccated and stored for long durations, and, hence, colonies must be reared continuously. In this protocol, we discuss methods for egg collection, including individual and en masse oviposition; egg disinfection to avoid the transmission of infectious agents to the next generation; and egg hatching for colony maintenance or experimentation. We also include optional methods for estimating life history traits such as fecundity, fertility, and larval mortality rates from egg counts. (© 2024 Cold Spring Harbor Laboratory Press.) |
| تواريخ الأحداث: | Date Created: 20230509 Date Completed: 20240304 Latest Revision: 20240515 |
| رمز التحديث: | 20240515 |
| DOI: | 10.1101/pdb.prot108186 |
| PMID: | 37160330 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1559-6095 |
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| DOI: | 10.1101/pdb.prot108186 |