دورية أكاديمية
Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism.
| العنوان: | Post-feeding Molecular Responses of Cobia (Rachycentron canadum): RNA-Sequencing as a Tool to Evaluate Postprandial Effects in Hepatic Lipid Metabolism. |
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| المؤلفون: | Araújo BC; Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, São Paulo, 08701-970, Brazil. brunocavar@usp.br., Barbosa DA; Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, São Paulo, 08701-970, Brazil., Honji RM; Centro de Biologia Marinha da, Universidade de São Paulo (CEBIMar/USP, Rodovia Manoel Hipólito Do Rego, Km 131, 5, São Sebastião, São Paulo, 11612-109, Brazil., Branco GS; Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, São Paulo, 05508-090, Brazil., Menegidio FB; Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, São Paulo, 08701-970, Brazil., Marques VH; Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, São Paulo, 05508-090, Brazil., Moreira RG; Departamento de Fisiologia, Instituto de Biociências, Universidade de São Paulo, São Paulo, São Paulo, 05508-090, Brazil., Kitahara MV; Centro de Biologia Marinha da, Universidade de São Paulo (CEBIMar/USP, Rodovia Manoel Hipólito Do Rego, Km 131, 5, São Sebastião, São Paulo, 11612-109, Brazil., Rombenso AN; CSIRO Agriculture and Food, Livestock & Aquaculture Program, Bribie Island Research Center, Woorim, QLD, 4507, Australia., de Mello PH; Beacon Development Company, King Abdullah University of Science and Technology (KAUST), Thuwal, Kingdom of Saudi Arabia., Hilsdorf AWS; Núcleo Integrado de Biotecnologia, Universidade de Mogi das Cruzes, Mogi das Cruzes, São Paulo, 08701-970, Brazil. |
| المصدر: | Marine biotechnology (New York, N.Y.) [Mar Biotechnol (NY)] 2023 Jun; Vol. 25 (3), pp. 358-371. Date of Electronic Publication: 2023 May 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer-Verlag New York Inc Country of Publication: United States NLM ID: 100892712 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1436-2236 (Electronic) Linking ISSN: 14362228 NLM ISO Abbreviation: Mar Biotechnol (NY) Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, NY : Springer-Verlag New York Inc., c1999- |
| مواضيع طبية MeSH: | Fatty Acids, Omega-3*/metabolism , Perciformes*/genetics , Perciformes*/metabolism, Animals ; Lipid Metabolism/genetics ; Fatty Acids/metabolism ; Eicosapentaenoic Acid ; Fishes/metabolism ; Liver/metabolism ; RNA/metabolism |
| مستخلص: | We used transcriptome sequencing to investigate the hepatic postprandial responses of Rachycentron canadum (cobia), an important commercial fish species. In total, 150 cobia juveniles (50 per tank, triplicate) were fed ad libitum with a commercial diet for 7 days, fasted for 24 h, and fed for 10 min. The liver was sampled 10 min prior to feeding and 30 min, 1, 2, 4, 8, 12, and 24 h after the feeding event. Each sample was evaluated in terms of liver fatty acid profile and gene expression. Differential gene expressions were evaluated, focusing on fatty acid synthesis and oxidation pathways. In general, the liver fatty acid profile reflected diet composition. Docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), and n-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) levels increased at 8 to 12 h but decreased at 24 h after the feeding event. A high number of differentially expressed genes (DEGs) were observed comparing fish that fasted for 8 h with those fasted for 30 min and 24 h, while a reduced number of DEGs was observed comparing individuals who fasted for 30 min compared with those who fasted for 24 h. Similarly, the main differences in the expression of genes related to the fatty acid biosynthesis and oxidation pathways were noticed in individuals who fasted for 8 h compared with those who fasted for 30 min and 24 h. The results suggested that the adequate time to sample the individuals ranged between 8 and 12 h after the meal since, apparently, after 24 h, differential gene expression was not necessarily influenced by food intake. (© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.) |
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| معلومات مُعتمدة: | 2016/12435-0 Fundação de Amparo à Pesquisa do Estado de São Paulo; 88882.365823/2019-01 Fundação Coordenação de Projetos, Pesquisas e Estudos Tecnológicos; 305493/2019-1 Conselho Nacional de Desenvolvimento Científico e Tecnológico; 304662/2017-8 Conselho Nacional de Desenvolvimento Científico e Tecnológico |
| فهرسة مساهمة: | Keywords: Fasting; Fatty acid oxidation; Fatty acid synthesis; Gene expression; RNA-seq |
| المشرفين على المادة: | 0 (Fatty Acids, Omega-3) 0 (Fatty Acids) AAN7QOV9EA (Eicosapentaenoic Acid) 63231-63-0 (RNA) |
| تواريخ الأحداث: | Date Created: 20230510 Date Completed: 20230706 Latest Revision: 20230706 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/s10126-023-10209-4 |
| PMID: | 37162622 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1436-2236 |
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| DOI: | 10.1007/s10126-023-10209-4 |