دورية أكاديمية
A Simple Method for the Detection of Wybutosine-Modified tRNA Phe GAA as a Readout of Retrograde tRNA Nuclear Import and Re-export: HCl/Aniline Cleavage and Nonradioactive Northern Hybridization.
| العنوان: | A Simple Method for the Detection of Wybutosine-Modified tRNA Phe |
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| المؤلفون: | Nostramo RT; Department of Molecular Genetics, Center for RNA Biology, The Ohio State University, Columbus, OH, USA., Hopper AK; Department of Molecular Genetics, Center for RNA Biology, The Ohio State University, Columbus, OH, USA. hopper.64@osu.edu. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2023; Vol. 2666, pp. 1-14. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | RNA, Transfer, Phe*/metabolism , Saccharomyces cerevisiae Proteins*/genetics , Saccharomyces cerevisiae Proteins*/metabolism, Active Transport, Cell Nucleus ; Hydrochloric Acid ; Saccharomyces cerevisiae/genetics ; Saccharomyces cerevisiae/metabolism ; RNA, Transfer/genetics ; RNA, Transfer/metabolism ; Cell Nucleus/genetics ; Cell Nucleus/metabolism |
| مستخلص: | tRNAs are highly mobile molecules that are trafficked back and forth between the nucleus and cytoplasm by several proteins. However, characterization of the movement of tRNAs and the proteins mediating these movements can be difficult. Here, we describe an easy and cost-effective assay to discover genes that are involved in two specific tRNA trafficking events, retrograde nuclear import and nuclear re-export for yeast, Saccharomyces cerevisiae. This assay, referred to as the hydrochloric acid (HCl)/aniline assay, identifies the presence or absence of a unique modification on tRNA Phe (© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.) |
| References: | EMBO J. 1987 Feb;6(2):477-83. (PMID: 3556165) Yeast. 2013 Apr;30(4):119-28. (PMID: 23417998) Genes Dev. 2015 Dec 15;29(24):2633-44. (PMID: 26680305) Nucleic Acids Res. 1974 Feb;1(2):183-92. (PMID: 4606505) Eur J Biochem. 1968 Sep 24;5(4):546-55. (PMID: 5698615) Nucleic Acids Res. 2020 Nov 18;48(20):11577-11588. (PMID: 33074312) Nucleic Acids Res. 1997 Jul 15;25(14):2694-701. (PMID: 9207014) Mol Cell Biol. 1998 Nov;18(11):6374-86. (PMID: 9774653) Genes Dev. 2018 Oct 1;32(19-20):1309-1314. (PMID: 30228203) Chem Rev. 1998 May 7;98(3):1109-1152. (PMID: 11848927) EMBO J. 2006 May 17;25(10):2142-54. (PMID: 16642040) Proc Natl Acad Sci U S A. 2005 Aug 9;102(32):11290-5. (PMID: 16040803) RNA. 2012 Oct;18(10):1921-33. (PMID: 22912484) Biochim Biophys Acta Gene Regul Mech. 2018 Apr;1861(4):373-386. (PMID: 29191733) Proc Natl Acad Sci U S A. 2011 Jun 28;108(26):10502-7. (PMID: 21670254) Genes Dev. 2017 Nov 1;31(21):2186-2198. (PMID: 29212662) EMBO J. 2002 Apr 2;21(7):1811-20. (PMID: 11927565) Elife. 2015 Apr 08;4:. (PMID: 25853343) Mol Biol Cell. 2003 Aug;14(8):3266-79. (PMID: 12925762) Genes Cells. 2007 Mar;12(3):285-97. (PMID: 17352735) |
| معلومات مُعتمدة: | R01 GM122884 United States GM NIGMS NIH HHS |
| فهرسة مساهمة: | Keywords: Aniline; Hydrochloric acid; Northern blot; Wybutosine; Yeast; tRNA re-export; tRNA retrograde nuclear import |
| المشرفين على المادة: | 0 (RNA, Transfer, Phe) 0 (wybutosine) QTT17582CB (Hydrochloric Acid) 9014-25-9 (RNA, Transfer) 0 (Saccharomyces cerevisiae Proteins) |
| تواريخ الأحداث: | Date Created: 20230511 Date Completed: 20230515 Latest Revision: 20230727 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10370157 |
| DOI: | 10.1007/978-1-0716-3191-1_1 |
| PMID: | 37166653 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-0716-3191-1_1 |