دورية أكاديمية
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Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis.

التفاصيل البيبلوغرافية
العنوان: Exploring linker's sequence diversity to fuse carotene cyclase and hydroxylase for zeaxanthin biosynthesis.
المؤلفون: Bouin A; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore.; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France., Zhang C; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore., Lindley ND; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore.; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France., Truan G; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France., Lautier T; Singapore Institute of Food and Biotechnology Innovation (SIFBI), Agency for Science, Technology and Research (A*STAR), Singapore.; TBI, Université de Toulouse, CNRS, INRAE, INSA, Toulouse, France.; CNRS@CREATE, 1 Create Way, #08-01 Create Tower, 138602, Singapore.
المصدر: Metabolic engineering communications [Metab Eng Commun] 2023 Apr 24; Vol. 16, pp. e00222. Date of Electronic Publication: 2023 Apr 24 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier B.V Country of Publication: Netherlands NLM ID: 101642117 Publication Model: eCollection Cited Medium: Internet ISSN: 2214-0301 (Electronic) Linking ISSN: 22140301 NLM ISO Abbreviation: Metab Eng Commun Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Amsterdam] : Elsevier B.V., [2014]-
مستخلص: Fusion of catalytic domains can accelerate cascade reactions by bringing enzymes in close proximity. However, the design of a protein fusion and the choice of a linker are often challenging and lack of guidance. To determine the impact of linker parameters on fusion proteins, a library of linkers featuring various lengths, secondary structures, extensions and hydrophobicities was designed. Linkers were used to fuse the lycopene cyclase (crtY) and β-carotene hydroxylase (crtZ) from Pantoea ananatis to create fusion proteins to produce zeaxanthin. The fusion efficiency was assessed by comparing the carotenoids content in a carotenoid-producer Escherichia coli strain. It was shown that in addition to the orientation of the enzymes and the size of the linker, the first amino acid of the linker is also a key factor in determining the efficiency of a protein fusion. The wide range of sequence diversity in our linker library enables the fine tuning of protein fusion and this approach can be easily transferred to other enzyme couples.
Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(© 2023 The Authors.)
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فهرسة مساهمة: Keywords: Artificial protein fusion; Linker; Protein engineering; Zeaxanthin
تواريخ الأحداث: Date Created: 20230511 Latest Revision: 20230514
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10165439
DOI: 10.1016/j.mec.2023.e00222
PMID: 37168436
قاعدة البيانات: MEDLINE
الوصف
تدمد:2214-0301
DOI:10.1016/j.mec.2023.e00222