دورية أكاديمية
In vivo experimentally study the effect of Nigella Sativa silver nanoparticles for treatment of salmonella species causing diarrhea in ruminants.
| العنوان: | In vivo experimentally study the effect of Nigella Sativa silver nanoparticles for treatment of salmonella species causing diarrhea in ruminants. |
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| المؤلفون: | Abdalhamed AM; Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt. Electronic address: am.abdelhamed@nrc.sci.eg., Zeedan GSG; Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt. Electronic address: gs.zeedan@nrc.sci.eg., Dorgham SM; Department of Microbiology and Immunology, National Research Centre, Dokki, Egypt. Electronic address: sohad_dorgham@yahoo.com., Ghazy AA; Department of Parasitology and Animal Diseases, National Research Centre, Dokki, Egypt. Electronic address: aaghazy7@hotmail.com. |
| المصدر: | Microbial pathogenesis [Microb Pathog] 2023 Jul; Vol. 180, pp. 106133. Date of Electronic Publication: 2023 May 10. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: England NLM ID: 8606191 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-1208 (Electronic) Linking ISSN: 08824010 NLM ISO Abbreviation: Microb Pathog Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London ; Orlando : Academic Press, c1986- |
| مواضيع طبية MeSH: | Nigella sativa*/chemistry , Metal Nanoparticles*/chemistry , Anti-Infective Agents* , Salmonella Infections*, Rats ; Animals ; Silver/pharmacology ; Silver/chemistry ; Anti-Bacterial Agents/pharmacology ; Anti-Bacterial Agents/therapeutic use ; Anti-Bacterial Agents/chemistry ; Rats, Wistar ; Salmonella ; Ruminants ; Diarrhea/drug therapy ; Diarrhea/veterinary ; Microbial Sensitivity Tests |
| مستخلص: | Salmonellais one of the main etiological agents of infectious diarrhea in large and small ruminants but emergence of multidrug-resistant (MDR) strains faster rate than previously, leads to develop of MDR strains among animals needs different alternative therapeutic strategies. Our study was aimed to evaluate the effects of Nigella sativa silver nanoparticles (NS AgNPs) on specific pathogen-free (SPF) Wister rats. Nigella sativa silver nanoparticles were prepared and confirmed their formation by optical observations, UV-Vis spectroscopy, transmission electron microscopy (TEM), and scanning electron microscopy (SEM). Rats in group G2 were infected experimentally with Salmonella spp and treated with ciprofloxacin orally for duration of 6 days at a dose rat 10 mg/kg. On the other hand, rats in group G1 were infected with salmonella and treated for 20 days with NS AgNPs in oral dose of (10 mg/kg rats), and the results were compared to control groups G3 which received bacterial infection without treatment and G4 control negative. The results of optical observation, UV-Vis spectroscopy, TEM, and SEM revealed typical characteristics of prepared NS AgNPs. Liver, kidney function biomarkers, hematologic analysis, and histological examination the tissues of liver, kidney, and stomach of rat's model improved that NS AgNPs has antimicrobial effect and has the ability to decrease the inflammatory reaction caused by Salmonella spp infection. The results of our study indicate that NS AgNPs are effective in controlling MDR Salmonella spp in vivo without causing any adverse effects. Moreover, our findings suggest that reducing the use of antimicrobials could be a key factor in the fight against antimicrobial resistance and can provide valuable insights into identifying the most appropriate treatment strategies to tackle this issue effectively in the future. Competing Interests: Declaration of competing interest The authors declare that they have no competing interests. (Copyright © 2023 Elsevier Ltd. All rights reserved.) |
| فهرسة مساهمة: | Keywords: In vivo; Nigella sativa; Salmonella; Silver nanoparticles |
| المشرفين على المادة: | 3M4G523W1G (Silver) 0 (Anti-Bacterial Agents) 0 (Anti-Infective Agents) |
| تواريخ الأحداث: | Date Created: 20230512 Date Completed: 20230606 Latest Revision: 20230606 |
| رمز التحديث: | 20230606 |
| DOI: | 10.1016/j.micpath.2023.106133 |
| PMID: | 37172661 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1096-1208 |
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| DOI: | 10.1016/j.micpath.2023.106133 |