دورية أكاديمية
أعرض في EDS

Visual Detection of Stem-Loop Primer Amplification (SPA) Products without Denaturation Using Peroxidase-like DNA Machines (PxDM).

التفاصيل البيبلوغرافية
العنوان: Visual Detection of Stem-Loop Primer Amplification (SPA) Products without Denaturation Using Peroxidase-like DNA Machines (PxDM).
المؤلفون: Maltzeva YI; Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, 191002 St. Petersburg, Russia., Gorbenko DA; Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, 191002 St. Petersburg, Russia., Nikitina EV; Pediatric Research and Clinical Center for Infectious Diseases, 197022 St. Petersburg, Russia., Rubel MS; Laboratory of Solution Chemistry of Advanced Materials and Technologies, ITMO University, 191002 St. Petersburg, Russia., Kolpashchikov DM; Chemistry Department, University of Central Florida, 4000 Central Florida Boulevard, Orlando, FL 32816, USA.; Burnett School of Biomedical Sciences, University of Central Florida, Orlando, FL 32816, USA.; National Center for Forensic Science, University of Central Florida, Orlando, FL 32826, USA.
المصدر: International journal of molecular sciences [Int J Mol Sci] 2023 Apr 25; Vol. 24 (9). Date of Electronic Publication: 2023 Apr 25.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, [2000-
مواضيع طبية MeSH: Peroxidase* , Nucleic Acids*, DNA ; Nucleic Acid Amplification Techniques/methods ; Polymerase Chain Reaction/methods ; Peroxidases ; Sensitivity and Specificity
مستخلص: Rapid, inexpensive, and accurate determination of nucleic acids is a decisive factor in evaluating population's health and monitoring treatment at point-of-care (POC) settings. Testing systems with visual outputs can provide instrument-free signal detection. Isothermal amplification technologies can substitute conventional polymerase chain reaction (PCR) testing due to compatibility with the POC diagnostic. Here, we have visually detected DNA fragments obtained by stem-loop-primer-assisted isothermal amplification (SPA), but not those obtained by PCR or LAMP amplification using DNA nanomachines with peroxidase-like activity (PxDM) with sensitivity to a single nucleotide substitution. Compared to the diagnostics with conventional loop-mediated isothermal amplification (LAMP), the PxDM method produces no false positive signals with the non-specific amplification products. The study suggests that PxDM, in conjunction with SPA isothermal amplification, can become a valid platform for POC testing systems.
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معلومات مُعتمدة: 22-75-10073 Russian Science Foundation
فهرسة مساهمة: Keywords: DNA-nanomachine; G-quadruplex; detection of dsDNA amplicons; isothermal amplification; point-of-care diagnostics; single nucleotide selectivity
المشرفين على المادة: EC 1.11.1.7 (Peroxidase)
9007-49-2 (DNA)
0 (Nucleic Acids)
EC 1.11.1.- (Peroxidases)
تواريخ الأحداث: Date Created: 20230513 Date Completed: 20230515 Latest Revision: 20231102
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10177805
DOI: 10.3390/ijms24097812
PMID: 37175522
قاعدة البيانات: MEDLINE
الوصف
تدمد:1422-0067
DOI:10.3390/ijms24097812