دورية أكاديمية
Membrane Permeability Is Required for the Vasodilatory Effect of Carbonic Anhydrase Inhibitors in Porcine Retinal Arteries.
| العنوان: | Membrane Permeability Is Required for the Vasodilatory Effect of Carbonic Anhydrase Inhibitors in Porcine Retinal Arteries. |
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| المؤلفون: | Eysteinsson T; Department of Physiology, BioMedical Center, Faculty of Medicine, University of Iceland, IS101 Reykjavik, Iceland., García-Llorca A; Department of Physiology, BioMedical Center, Faculty of Medicine, University of Iceland, IS101 Reykjavik, Iceland., Hardarson AO; Department of Physiology, BioMedical Center, Faculty of Medicine, University of Iceland, IS101 Reykjavik, Iceland., Vullo D; Sezione di Scienze Farmaceutiche e Nutraceutiche, Neurofarba Department, University of Florence, Via Ugo Schiff 6, 50019 Florence, Italy., Carta F; Sezione di Scienze Farmaceutiche e Nutraceutiche, Neurofarba Department, University of Florence, Via Ugo Schiff 6, 50019 Florence, Italy., Supuran CT; Sezione di Scienze Farmaceutiche e Nutraceutiche, Neurofarba Department, University of Florence, Via Ugo Schiff 6, 50019 Florence, Italy. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2023 May 02; Vol. 24 (9). Date of Electronic Publication: 2023 May 02. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Carbonic Anhydrase Inhibitors*/pharmacology , Retinal Artery*, Animals ; Swine ; Vasodilation ; Benzolamide/pharmacology ; Permeability |
| مستخلص: | It has been demonstrated previously that a variety of carbonic anhydrase inhibitors (CAIs) can induce vasodilation in pre-contracted retinal arteriolar segments although with different efficacy and potency. Since the CAIs tested so far are able to permeate cell membranes and inhibit both intracellular and extracellular isoforms of the enzyme, it is not clear whether extra- or intracellular isoforms or mechanisms are mediating their vasodilatory effects. By means of small wire myography, we have tested the effects of four new CAIs on wall tension in pre-contracted retinal arteriolar segments that demonstrably do not enter cell membranes but have high affinity to both cytosolic and membrane-bound isoforms of CA. At concentrations between 10 -6 M to 10 -3 M, none of the four membrane impermeant CAIs had any significant effect on arteriolar wall tension, while the membrane permeant CAI benzolamide (10 -3 M) fully dilated all arteriolar segments tested. This suggests that CAI act as vasodilators through cellular mechanisms located in the cytoplasm of vascular cells. |
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| معلومات مُعتمدة: | 101 Helga Jónsdóttir and Sigurlidi Kristjánsson Memorial Fund; University of Iceland Research Fund |
| فهرسة مساهمة: | Keywords: arterial wall tension; carbonic anhydrase; membrane impermeable; myography; porcine |
| المشرفين على المادة: | 0 (Carbonic Anhydrase Inhibitors) FC5AAH89R5 (Benzolamide) |
| تواريخ الأحداث: | Date Created: 20230513 Date Completed: 20230515 Latest Revision: 20230515 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10179589 |
| DOI: | 10.3390/ijms24098140 |
| PMID: | 37175846 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1422-0067 |
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| DOI: | 10.3390/ijms24098140 |