دورية أكاديمية
Tissue factor as a novel diagnostic target for early detection of ovarian cancer using ultrasound microbubbles.
| العنوان: | Tissue factor as a novel diagnostic target for early detection of ovarian cancer using ultrasound microbubbles. |
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| المؤلفون: | Newcomer MM; Division of Anatomy, College of Medicine, The Ohio State University, Columbus, OH, USA; Department of Anatomy, School of Medicine, Case Western Reserve University, Cleveland, OH, USA., Dorayappan KDP; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Wagner V; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Suarez AA; Department of Pathology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Calo CA; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Kalmar EL; Division of Anatomy, College of Medicine, The Ohio State University, Columbus, OH, USA., Maxwell GL; Inova Women's Service Line and the Inova Schar Cancer Institute, Falls Church, VA, USA., O'Malley D; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Cohn DE; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA., Tweedle MF; Department of Radiology, Comprehensive Cancer Center, The Ohio State University, USA., Selvendiran K; Division of Gynecologic Oncology, Comprehensive Cancer Center, The Ohio State University Wexner Medical Center, Columbus, OH, USA.. Electronic address: selvendiran.karuppaiyah@osumc.edu. |
| المصدر: | Gynecologic oncology [Gynecol Oncol] 2023 Jun; Vol. 173, pp. 138-150. Date of Electronic Publication: 2023 May 11. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Academic Press Country of Publication: United States NLM ID: 0365304 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1095-6859 (Electronic) Linking ISSN: 00908258 NLM ISO Abbreviation: Gynecol Oncol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York, Academic Press. |
| مواضيع طبية MeSH: | Microbubbles* , Ovarian Neoplasms*/diagnostic imaging , Ovarian Neoplasms*/metabolism, Humans ; Mice ; Female ; Animals ; Thromboplastin ; Endothelial Cells/metabolism ; Early Detection of Cancer ; Ultrasonography/methods |
| مستخلص: | Introduction: Ovarian cancer (OC) is the deadliest gynecologic malignancy, with an overall 5-year survival rate of less than 30%. The existing paradigm for OC detection involves a serum marker, CA125, and ultrasound examination, neither of which is sufficiently specific for OC. This study addresses this deficiency through the use of a targeted ultrasound microbubble directed against tissue factor (TF). Methods: TF expression was examined in both OC cell lines and patient-derived tumor samples via western blotting and IHC. In vivo microbubble ultrasound imaging was analyzed using high grade serous ovarian carcinoma orthotopic mouse models. Results: While TF expression has previously been described on angiogenic, tumor-associated vascular endothelial cells (VECs) of several tumor types, this is first study to show TF expression on both murine and patient-derived ovarian tumor-associated VECs. Biotinylated anti-TF antibody was conjugated to streptavidin-coated microbubbles and in vitro binding assays were performed to assess the binding efficacy of these agents. TF-targeted microbubbles successfully bound to TF-expressing OC cells, as well as an in vitro model of angiogenic endothelium. In vivo, these microbubbles bound to the tumor-associated VECs of a clinically relevant orthotopic OC mouse model. Conclusion: Development of a TF-targeted microbubble capable of successfully detecting ovarian tumor neovasculature could have significant implications towards increasing the number of early-stage OC diagnoses. This preclinical study shows potential for translation to clinical use, which could ultimately help increase the number of early OC detections and decrease the mortality associated with this disease. (Copyright © 2023. Published by Elsevier Inc.) |
| فهرسة مساهمة: | Keywords: Early detection; Ovarian cancer; Tissue factor; Ultrasound microbubbles; Vascular endothelial cells |
| المشرفين على المادة: | 9035-58-9 (Thromboplastin) |
| تواريخ الأحداث: | Date Created: 20230513 Date Completed: 20230602 Latest Revision: 20230607 |
| رمز التحديث: | 20230608 |
| DOI: | 10.1016/j.ygyno.2023.04.008 |
| PMID: | 37178671 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1095-6859 |
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| DOI: | 10.1016/j.ygyno.2023.04.008 |