دورية أكاديمية
أعرض في EDS

Neural mechanisms of parasite-induced summiting behavior in 'zombie' Drosophila .

التفاصيل البيبلوغرافية
العنوان: Neural mechanisms of parasite-induced summiting behavior in 'zombie' Drosophila .
المؤلفون: Elya C; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Lavrentovich D; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Lee E; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Pasadyn C; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Duval J; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Basak M; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., Saykina V; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States., de Bivort B; Department of Organismic and Evolutionary Biology, Harvard University, Cambridge, United States.
المصدر: ELife [Elife] 2023 May 15; Vol. 12. Date of Electronic Publication: 2023 May 15.
نوع المنشور: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Drosophila* , Parasites*, Animals ; Drosophila melanogaster/physiology ; Corpora Allata/metabolism ; Juvenile Hormones/metabolism
مستخلص: For at least two centuries, scientists have been enthralled by the "zombie" behaviors induced by mind-controlling parasites. Despite this interest, the mechanistic bases of these uncanny processes have remained mostly a mystery. Here, we leverage the Entomophthora muscae - Drosophila melanogaster "zombie fly" system to reveal the mechanistic underpinnings of summit disease, a manipulated behavior evoked by many fungal parasites. Using a high-throughput approach to measure summiting, we discovered that summiting behavior is characterized by a burst of locomotion and requires the host circadian and neurosecretory systems, specifically DN1p circadian neurons, pars intercerebralis to corpora allata projecting (PI-CA) neurons and corpora allata (CA), the latter being solely responsible for juvenile hormone (JH) synthesis and release. Using a machine learning classifier to identify summiting animals in real time, we observed that PI-CA neurons and CA appeared intact in summiting animals, despite invasion of adjacent regions of the "zombie fly" brain by E. muscae cells and extensive host tissue damage in the body cavity. The blood-brain barrier of flies late in their infection was significantly permeabilized, suggesting that factors in the hemolymph may have greater access to the central nervous system during summiting. Metabolomic analysis of hemolymph from summiting flies revealed differential abundance of several compounds compared to non-summiting flies. Transfusing the hemolymph of summiting flies into non-summiting recipients induced a burst of locomotion, demonstrating that factor(s) in the hemolymph likely cause summiting behavior. Altogether, our work reveals a neuro-mechanistic model for summiting wherein fungal cells perturb the fly's hemolymph, activating a neurohormonal pathway linking clock neurons to juvenile hormone production in the CA, ultimately inducing locomotor activity in their host.
Competing Interests: CE, DL, EL, CP, JD, MB, VS, Bd No competing interests declared
(© 2023, Elya et al.)
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معلومات مُعتمدة: R01 NS121874 United States NS NINDS NIH HHS; GT11087 United States HHMI Howard Hughes Medical Institute
فهرسة مساهمة: Keywords: D. melanogaster; Entomophthora muscae; behavior; circadian rhythm; genetics; genomics; juvenile hormone; neuroscience; parasitic mind-control; summit disease
المشرفين على المادة: 0 (Juvenile Hormones)
تواريخ الأحداث: Date Created: 20230515 Date Completed: 20230609 Latest Revision: 20240701
رمز التحديث: 20240701
مُعرف محوري في PubMed: PMC10259475
DOI: 10.7554/eLife.85410
PMID: 37184212
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.85410