دورية أكاديمية
أعرض في EDS

Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel.

التفاصيل البيبلوغرافية
العنوان: Anti-inflammatory, procollagen, and wound repair properties of topical insulin gel.
المؤلفون: Apolinário PP; Colégio Técnico de Campinas, Universidade Estadual de Campinas, Campinas, SP, Brasil., Zanchetta FC; Faculdade de Enfermagem, Universidade Estadual de Campinas, Campinas, SP, Brasil., Breder JSC; Faculdade de Enfermagem, Universidade Estadual de Campinas, Campinas, SP, Brasil., Adams G; Faculty of Medicine and Health Science, University of Nottingham, Nottingham, UK., Consonni SR; Insituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil., Gillis R; Department of Service Sector Management, Sheffield Hallam University, Sheffield, UK., Saad MJA; Faculdade de Ciências Médicas, Universidade Estadual de Campinas, Campinas, SP, Brasil., Lima MHM; Faculdade de Enfermagem, Universidade Estadual de Campinas, Campinas, SP, Brasil.
المصدر: Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas [Braz J Med Biol Res] 2023 May 15; Vol. 56, pp. e12640. Date of Electronic Publication: 2023 May 15 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Associação Brasileira de Divulgação Científica Country of Publication: Brazil NLM ID: 8112917 Publication Model: eCollection Cited Medium: Internet ISSN: 1414-431X (Electronic) Linking ISSN: 0100879X NLM ISO Abbreviation: Braz J Med Biol Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [SP, Brasil : Associação Brasileira de Divulgação Científica, 1981-
مواضيع طبية MeSH: Insulin* , Procollagen*, Mice ; Animals ; Mice, Obese ; Wound Healing ; Anti-Inflammatory Agents
مستخلص: Diabetes mellitus is associated with impaired wound healing. The topical use of insulin is a promising therapy because it may favor all phases of the wound healing process. This study aimed to investigate the therapeutic outcomes of insulin gel in wounds of hyperglycemic mice. After diabetes induction, a 1-cm2 full-thickness wound was created on each animal's dorsum. The lesions were treated daily for 14 days with insulin gel (insulin group) or vehicle gel without insulin (vehicle group). Tissue samples were extracted on days 4, 7, 10, and 14 after the creation of the lesion. The samples were analyzed with hematoxylin/eosin and Sirius red staining, immunohistochemistry, Bio-Plex immunoassays, and western blotting. Insulin gel favored re-epithelialization at day 10 and increased the organization and deposition of collagen. Additionally, it modulated the expression of cytokines (interleukin (IL)-4 and IL-10) and increased the expression of arginase I, VEGF receptor 1, and VEGF on day 10. Activation of the insulin signaling pathway occurred via IRβ, IRS1, and IKK on day 10 and activation of Akt and IRS1 on day 14. These results suggested that insulin gel improved wound healing in hyperglycemic mice by modulating the expression of inflammatory factors, growth factors, and proteins of the insulin signaling pathway.
References: Can J Diabetes. 2018 Apr;42 Suppl 1:S10-S15. (PMID: 29650080)
Innate Immun. 2016 Nov;22(8):647-657. (PMID: 27670945)
J Clin Endocrinol Metab. 2001 Mar;86(3):972-9. (PMID: 11238471)
Adv Wound Care (New Rochelle). 2018 Jul 1;7(7):209-231. (PMID: 29984112)
Sci Rep. 2021 Jan 14;11(1):1474. (PMID: 33446687)
Nature. 2008 May 15;453(7193):314-21. (PMID: 18480812)
Mol Cell Biol. 2003 Aug;23(16):5726-37. (PMID: 12897144)
PLoS One. 2012;7(5):e36974. (PMID: 22662132)
PLoS One. 2017 Nov 7;12(11):e0187670. (PMID: 29112962)
Diabetes Metab Syndr Obes. 2020 Mar 13;13:719-727. (PMID: 32214835)
Int J Low Extrem Wounds. 2015 Dec;14(4):353-64. (PMID: 26349856)
Front Immunol. 2018 Dec 04;9:2851. (PMID: 30564244)
Biol Open. 2018 Jan 17;7(1):. (PMID: 29101099)
Sci Signal. 2009 Aug 04;2(82):ra41. (PMID: 19654415)
Cell. 1994 Mar 25;76(6):959-62. (PMID: 8137429)
Biol Res Nurs. 2016 Mar;18(2):181-92. (PMID: 26111834)
Psychol Bull. 1992 Jul;112(1):155-9. (PMID: 19565683)
J Invest Dermatol. 2009 Mar;129(3):752-64. (PMID: 18668138)
Front Physiol. 2018 May 01;9:419. (PMID: 29765329)
Curr Protoc Pharmacol. 2015 Sep 01;70:5.47.1-5.47.20. (PMID: 26331889)
Biomed Pharmacother. 2019 Apr;112:108615. (PMID: 30784919)
Front Bioeng Biotechnol. 2021 Sep 20;9:718377. (PMID: 34616718)
Biochemistry (Mosc). 2016 Nov;81(11):1251-1260. (PMID: 27914451)
Gene. 2009 Apr 15;435(1-2):36-44. (PMID: 19393188)
J Surg Res. 2002 Jun 1;105(1):35-42. (PMID: 12069499)
Bioengineering (Basel). 2021 May 11;8(5):. (PMID: 34064689)
J Wound Care. 1999 Nov;8(10):526-8. (PMID: 10827659)
Acta Biomater. 2018 Feb;67:183-195. (PMID: 29242162)
An Bras Dermatol. 2018 Jun;93(3):415-418. (PMID: 29924246)
J Biol Chem. 2013 Aug 23;288(34):24363-71. (PMID: 23843455)
Cell. 2007 Jun 29;129(7):1261-74. (PMID: 17604717)
J Am Assoc Lab Anim Sci. 2014 Nov;53(6):684-91. (PMID: 25650976)
Surgery. 1997 May;121(5):513-9. (PMID: 9142149)
J Eur Acad Dermatol Venereol. 2012 Jul;26(7):812-20. (PMID: 22211801)
Front Med (Lausanne). 2020 Aug 27;7:438. (PMID: 32974363)
J Cell Sci. 2020 Oct 12;133(19):. (PMID: 32878940)
J Dermatol Sci. 2009 Sep;55(3):170-8. (PMID: 19559571)
Pharmaceuticals (Basel). 2020 Apr 01;13(4):. (PMID: 32244718)
J Biochem Mol Biol. 2006 Sep 30;39(5):469-78. (PMID: 17002866)
المشرفين على المادة: 0 (Insulin)
0 (Procollagen)
0 (Anti-Inflammatory Agents)
تواريخ الأحداث: Date Created: 20230517 Date Completed: 20230518 Latest Revision: 20230607
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10241483
DOI: 10.1590/1414-431X2023e12640
PMID: 37194835
قاعدة البيانات: MEDLINE
الوصف
تدمد:1414-431X
DOI:10.1590/1414-431X2023e12640