دورية أكاديمية
Stem cells therapeutic effect in a reserpine-induced fibromyalgia rat model: A possible NLRP3 inflammasome modulation with neurogenesis promotion in the cerebral cortex.
| العنوان: | Stem cells therapeutic effect in a reserpine-induced fibromyalgia rat model: A possible NLRP3 inflammasome modulation with neurogenesis promotion in the cerebral cortex. |
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| المؤلفون: | Mokhemer SA; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, 61511 El-Minia, Egypt. Electronic address: sahar.ahmedmokhemer@mu.edu.eg., Desouky MK; Department of Anatomy, Faculty of Medicine, Minia University, 61511 El-Minia, Egypt., Abdelghany AK; Animal and Poultry Management and Wealth Development Department, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef 62511, Egypt., Ibrahim MFG; Department of Histology and Cell Biology, Faculty of Medicine, Minia University, 61511 El-Minia, Egypt. |
| المصدر: | Life sciences [Life Sci] 2023 Jul 15; Vol. 325, pp. 121784. Date of Electronic Publication: 2023 May 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Fibromyalgia*/chemically induced , Fibromyalgia*/therapy , Mesenchymal Stem Cells*/metabolism , Brain Injuries*/metabolism, Rats ; Animals ; Inflammasomes/metabolism ; NLR Family, Pyrin Domain-Containing 3 Protein/metabolism ; Reserpine ; Tumor Necrosis Factor-alpha/metabolism ; Beclin-1/metabolism ; Cerebral Cortex/metabolism ; Caspase 1/metabolism ; HMGB Proteins/metabolism |
| مستخلص: | Fibromyalgia is a chronic pain syndrome with a multifactorial pathophysiology affecting 2-8 % of the population. Aims: To investigate the therapeutic effects of bone marrow mesenchymal stem cells (BMSCs) against fibromyalgia-related cerebral cortex damage and the possible underlying mechanisms of action. Materials and Methods: Rats were randomly allocated into three groups; control, fibromyalgia and fibromyalgia treated with BMSCs groups. Physical and behavioural assessments were performed. Cerebral cortices were collected for biochemical and histological assessment. Key Findings: Fibromyalgia group showed behavioural changes indicating presence of pain, fatigue, depression, and sleep disturbances. Moreover, biochemical biomarkers alterations were demonstrated by a significant decrease in brain monoamines and GSH levels, but MDA, NO, TNF-alpha, HMGB-1, NLRP3, and caspase-1 levels significantly increased. Furthermore, histological assessment revealed structural and ultrastructural alterations indicating neuronal and neuroglial degeneration with microglia activation, an increase in mast cell number and IL-1β immune-expression. Additionally, a significant decrease in Beclin-1 immune-expression, and blood brain barrier disruption were noticed. Interestingly, BMSCs administration significantly improved behavioural alterations, restored the reduced brain monoamines and oxidative stress markers, and reduced TNF-alpha, HMGB-1, NLRP3, and caspase-1 levels. Profoundly, cerebral cortices demonstrated improved histological structure, significant decrease in mast cell number and IL-1β immune-expression, besides a significant increase in Beclin-1 and DCX immune-expression. Significance: For the best of our knowledge, this is the first study showing ameliorative effects for BMSCs treatment in fibromyalgia-related cerebral cortical damage. The neurotherapeutic effects of BMSCs could be attributed to NLRP3 inflammasome signaling pathway inhibition, mast cell deactivation, and stimulation of neurogenesis and autophagy. Competing Interests: Declaration of competing interest There is no conflict of interests disclosed. (Copyright © 2023 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: BMSCs; Beclin-1; DCX; Fibromyalgia; Mast cell; NLRP3 Inflammasome |
| المشرفين على المادة: | 0 (Inflammasomes) 0 (NLR Family, Pyrin Domain-Containing 3 Protein) 8B1QWR724A (Reserpine) 0 (Tumor Necrosis Factor-alpha) 0 (Beclin-1) EC 3.4.22.36 (Caspase 1) 0 (HMGB Proteins) 0 (Nlrp3 protein, rat) |
| تواريخ الأحداث: | Date Created: 20230517 Date Completed: 20230529 Latest Revision: 20230529 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.lfs.2023.121784 |
| PMID: | 37196857 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1879-0631 |
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| DOI: | 10.1016/j.lfs.2023.121784 |