دورية أكاديمية
أعرض في EDS

Whole genome sequencing of Ethiopian Brucella abortus isolates expands the known diversity of an early branching sub-Saharan African lineage.

التفاصيل البيبلوغرافية
العنوان: Whole genome sequencing of Ethiopian Brucella abortus isolates expands the known diversity of an early branching sub-Saharan African lineage.
المؤلفون: Edao BM; Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom.; College of Veterinary Medicine, Addis Ababa University, Bishoftu, Ethiopia., Ameni G; Aklilu Lemma Institute of Pathobiology, Addis Ababa University, Addis Ababa, Ethiopia.; Department of Veterinary Medicine, College of Food and Agriculture, United Arab Emirates University, Al Ain, United Arab Emirates., Berg S; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom., Tekle M; College of Veterinary Medicine, Addis Ababa University, Bishoftu, Ethiopia., Whatmore AM; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom., Wood JLN; Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom., van Tonder AJ; Department of Veterinary Medicine, University of Cambridge, Cambridge, United Kingdom., Ashford RT; Department of Bacteriology, Animal and Plant Health Agency, Weybridge, United Kingdom.
المصدر: Frontiers in microbiology [Front Microbiol] 2023 May 04; Vol. 14, pp. 1128966. Date of Electronic Publication: 2023 May 04 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Brucellosis remains one of the most significant zoonotic diseases globally, responsible for both considerable human morbidity and economic losses due to its impacts on livestock productivity. Despite this, there remain significant evidence gaps in many low- and middle-income countries, including those of sub-Saharan Africa. Here we report the first molecular characterisation of Brucella sp. from Ethiopia. Fifteen Brucella sp. isolates from an outbreak in cattle from a herd in central Ethiopia were identified as Brucella abortus , using bacterial culture and molecular methods. Sequencing of the Ethiopian B. abortus isolates allowed their phylogenetic comparison with 411 B. abortus strains of diverse geographical origins, using whole genome single nucleotide polymorphisms (wgSNP). The Ethiopian isolates belonged to an early-branching lineage (Lineage A) previously only represented by data from two strains, both of sub-Saharan African origin (Kenya and Mozambique). A second B. abortus lineage (Lineage B), also comprised solely of strains originating from sub-Saharan Africa, was identified. The majority of strains belonged to one of two lineages of strains originating from a much broader geographical range. Further analyses based on multi-locus sequence typing (MLST) and multi-locus variable-number tandem repeat analysis (MLVA) expanded the number of B. abortus strains available for comparison with the Ethiopian isolates and were consistent with the findings from wgSNP analysis. MLST profiles of the Ethiopian isolates expanded the sequence type (ST) diversity of the early branching lineage of B. abortus , equivalent to wgSNP Lineage A. A more diverse cluster of STs, equivalent to wgSNP Lineage B, was comprised solely of strains originating from sub-Saharan Africa. Similarly, analysis of B. abortus MLVA profiles ( n  = 1891) confirmed that the Ethiopian isolates formed a unique cluster, similar to only two existing strains, and distinct from the majority of other strains of sub-Saharan African origin. These findings expand the known diversity of an under-represented lineage of B. abortus and suggest a potential evolutionary origin for the species in East Africa. In addition to providing information concerning Brucella species extant within Ethiopia this work serves as the basis for further studies on the global population structure and evolutionary history of a major zoonotic pathogen.
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 Edao, Ameni, Berg, Tekle, Whatmore, Wood, van Tonder and Ashford.)
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فهرسة مساهمة: Keywords: Brucella abortus; lineage; molecular typing; sub-Saharan Africa; whole genome sequencing
تواريخ الأحداث: Date Created: 20230522 Latest Revision: 20230523
رمز التحديث: 20230523
مُعرف محوري في PubMed: PMC10192883
DOI: 10.3389/fmicb.2023.1128966
PMID: 37213520
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2023.1128966