دورية أكاديمية
Rapid simultaneous determination of thirteen aristolochic acids analogs in Aristolochiaceae plants by Ultra-High-Performance liquid Chromatography- tandem mass spectrometry in dynamic multiple reaction monitoring mode.
| العنوان: | Rapid simultaneous determination of thirteen aristolochic acids analogs in Aristolochiaceae plants by Ultra-High-Performance liquid Chromatography- tandem mass spectrometry in dynamic multiple reaction monitoring mode. |
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| المؤلفون: | Ji H; School of Pharmacy, Jiangsu Vocational College of Medicine, Yancheng 224005, China. Electronic address: hongjianji2006@163.com., Zhang G; School of Pharmacy, Changchun University of Chinese Medicine, Changchun 130117, China., Zhou X; Department of Nephrology, Sixth People's Hospital Affiliated to Nantong University, The Third People's Hospital of Yancheng, Yancheng 224001, China. |
| المصدر: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [J Chromatogr B Analyt Technol Biomed Life Sci] 2023 Jun 01; Vol. 1225, pp. 123753. Date of Electronic Publication: 2023 May 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101139554 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-376X (Electronic) Linking ISSN: 15700232 NLM ISO Abbreviation: J Chromatogr B Analyt Technol Biomed Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| مواضيع طبية MeSH: | Aristolochiaceae* , Aristolochic Acids*/analysis , Asarum*/chemistry , Aristolochia*/chemistry, Chromatography, High Pressure Liquid/methods ; Tandem Mass Spectrometry/methods |
| مستخلص: | Asarum and Aristolochia are two large genera of Aristolochiaceae plants containing typical toxicant aristolochic acid analogs(AAAs), AAAs can be deemed as toxicity markers of Aristolochiaceae plants. Based on the least AAAs in dry roots and rhizomes of Asarum heterotropoides, Asarum sieboldii Miq and Asarum sieboldii var, all of which are enrolled in the Chinese pharmacopeia up to now. AAAs distribution in Aristolochiaceae plants, especially Asarum L. plants, is still obscure and controversial due to few AAAs measured, unverified species of Asarum, and complicated pretreatment in analytical samples making the results more challenging to reproduce. In the present study, a simple ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) method in dynamic multiple reaction monitoring mode for simultaneous determination of thirteen AAAs was developed for evaluating the distribution of toxicity phytochemicals in Aristolochiaceae plants. The sample was prepared by extracting Asarum and Aristolochia powder with methanol, and the supernatant was analyzed using the Agilent 6410 system on an ACQUITY UPLC HSS PFP column with gradient elution of water and acetonitrile, containing 1% v/v formic acid (FA) each, at a flow rate of 0.3 mL/min. The chromatographic condition provided good peak shape and resolution. The method was linear over the specific ranges with the coefficient of determination (R 2 ) > 0.990. Satisfactory intra- and inter-day precisions were achieved with RSD less than 9.79%, and the average recovery factors obtained were in the range of 88.50%～105.49%%. The proposed method was successfully applied for simultaneous quantification of the 13 AAAs in 19 samples from 5 Aristolochiaceae species, especially three Asarum L. species enrolled in the Chinese Pharmacopoeia. Except Asarum heterotropoides, the results supported that the Chinese Pharmacopoeia (2020 Edition) adopting the root with rhizome as medicinal parts of Herba Asari instead of the whole herb for drug safety by providing scientific data. Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: [Hongjian ji reports article publishing charges was provided by Jiang su vocational college of medicine. Hongjian ji reports a relationship with Jiangsu Vocational College of Medicine that includes: funding grants. Hongjian ji has patent pending to Not Applicable. The authors state that they had no conflict of interests]. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Aristolochiaceae; Aristolochic Acids Analogs; Dynamic Multiple Reaction Monitoring Mode; UHPLC-MS/MS |
| المشرفين على المادة: | 0 (Aristolochic Acids) |
| تواريخ الأحداث: | Date Created: 20230522 Date Completed: 20230616 Latest Revision: 20230616 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.jchromb.2023.123753 |
| PMID: | 37216764 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-376X |
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| DOI: | 10.1016/j.jchromb.2023.123753 |