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Genetic and histopathological analysis of spermatogenesis after short-term testicular torsion in rats.

التفاصيل البيبلوغرافية
العنوان: Genetic and histopathological analysis of spermatogenesis after short-term testicular torsion in rats.
المؤلفون: Katami H; Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Suzuki S; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Fujii T; Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Ueno M; Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Tanaka A; Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Ohta KI; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Miki T; Department of Anatomy and Neurobiology, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan., Shimono R; Department of Pediatric Surgery, Faculty of Medicine, Kagawa University, Miki-cho, Kagawa Prefecture, Japan. shimono.ryuichi@kagawa-u.ac.jp.
المصدر: Pediatric research [Pediatr Res] 2023 Nov; Vol. 94 (5), pp. 1650-1658. Date of Electronic Publication: 2023 May 24.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: United States NLM ID: 0100714 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1530-0447 (Electronic) Linking ISSN: 00313998 NLM ISO Abbreviation: Pediatr Res Subsets: MEDLINE
أسماء مطبوعة: Publication: 2012- : New York : Nature Publishing Group
Original Publication: Basel ; New York : Karger.
مواضيع طبية MeSH: Spermatic Cord Torsion*/genetics , Reperfusion Injury*/genetics , Reperfusion Injury*/metabolism , Reperfusion Injury*/pathology, Humans ; Rats ; Male ; Animals ; Rats, Sprague-Dawley ; Semen/metabolism ; Spermatogenesis ; Testis/pathology ; Ischemia/genetics ; Ischemia/pathology
مستخلص: Background: Patients with testicular torsion (TT) may exhibit impaired spermatogenesis from reperfusion injury after detorsion surgery. Alteration in the expressions of spermatogenesis-related genes induced by TT have not been fully elucidated.
Methods: Eight-week-old Sprague-Dawley rats were grouped as follows: group 1 (sham-operated), group 2 (TT without reperfusion) and group 3 (TT with reperfusion). TT was induced by rotating the left testis 720° for 1 h. Testicular reperfusion proceeded for 24 h. Histopathological examination, oxidative stress biomarker measurements, RNA sequencing and RT-PCR were performed.
Results: Testicular ischemia/reperfusion injury induced marked histopathological changes. Germ cell apoptosis was significantly increased in group 3 compared with group 1 and 2 (mean apoptotic index: 26.22 vs. 0.64 and 0.56; p = 0.024, and p = 0.024, respectively). Johnsen score in group 3 was smaller than that in group 1 and 2 (mean: 8.81 vs 9.45 and 9.47 points/tubule; p = 0.001, p < 0.001, respectively). Testicular ischemia/reperfusion injury significantly upregulated the expression of genes associated with apoptosis and antioxidant enzymes and significantly downregulated the expression of genes associated with spermatogenesis.
Conclusion: One hour of TT followed by reperfusion injury caused histopathological testicular damage. The relatively high Johnsen score indicated spermatogenesis was maintained. Genes associated with spermatogenesis were downregulated in the TT rat model.
Impact: How ischemia/reperfusion injury in testicular torsion (TT) affects the expressions of genes associated with spermatogenesis has not been fully elucidated. This is the first study to report comprehensive gene expression profiles using next generation sequencing for an animal model of TT. Our results revealed that ischemia/reperfusion injury downregulated the expression of genes associated with spermatogenesis and sperm function in addition to histopathological damage, even though the duration of ischemia was short.
(© 2023. The Author(s), under exclusive licence to the International Pediatric Research Foundation, Inc.)
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تواريخ الأحداث: Date Created: 20230524 Date Completed: 20231106 Latest Revision: 20231213
رمز التحديث: 20231215
DOI: 10.1038/s41390-023-02638-7
PMID: 37225778
قاعدة البيانات: MEDLINE
الوصف
تدمد:1530-0447
DOI:10.1038/s41390-023-02638-7