دورية أكاديمية
أعرض في EDS

Genome sequence of Ophryocystis elektroscirrha, an apicomplexan parasite of monarch butterflies: cryptic diversity and response to host-sequestered plant chemicals.

التفاصيل البيبلوغرافية
العنوان: Genome sequence of Ophryocystis elektroscirrha, an apicomplexan parasite of monarch butterflies: cryptic diversity and response to host-sequestered plant chemicals.
المؤلفون: Mongue AJ; Department of Entomology and Nematology, University of Florida, Gainesville, USA. andrew.mongue@ufl.edu., Martin SH; Institute of Ecology and Evolution, University of Edinburgh, Edinburgh, UK., Manweiler REV; Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, USA., Scullion H; Department of Entomology and Nematology, University of Florida, Gainesville, USA., Koehn JL; Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, USA., de Roode JC; Biology Department, Emory University, Atlanta, USA., Walters JR; Department of Ecology and Evolutionary Biology, University of Kansas, Lawrence, USA.
المصدر: BMC genomics [BMC Genomics] 2023 May 24; Vol. 24 (1), pp. 278. Date of Electronic Publication: 2023 May 24.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100965258 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2164 (Electronic) Linking ISSN: 14712164 NLM ISO Abbreviation: BMC Genomics Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Butterflies*/genetics , Parasites* , Apicomplexa*/genetics, Animals ; Sodium-Potassium-Exchanging ATPase ; Sodium
مستخلص: Apicomplexa are ancient and diverse organisms which have been poorly characterized by modern genomics. To better understand the evolution and diversity of these single-celled eukaryotes, we sequenced the genome of Ophryocystis elektroscirrha, a parasite of monarch butterflies, Danaus plexippus. We contextualize our newly generated resources within apicomplexan genomics before answering longstanding questions specific to this host-parasite system. To start, the genome is miniscule, totaling only 9 million bases and containing fewer than 3,000 genes, half the gene content of two other sequenced invertebrate-infecting apicomplexans, Porospora gigantea and Gregarina niphandrodes. We found that O. elektroscirrha shares different orthologs with each sequenced relative, suggesting the true set of universally conserved apicomplexan genes is very small indeed. Next, we show that sequencing data from other potential host butterflies can be used to diagnose infection status as well as to study diversity of parasite sequences. We recovered a similarly sized parasite genome from another butterfly, Danaus chrysippus, that was highly diverged from the O. elektroscirrha reference, possibly representing a distinct species. Using these two new genomes, we investigated potential evolutionary response by parasites to toxic phytochemicals their hosts ingest and sequester. Monarch butterflies are well-known to tolerate toxic cardenolides thanks to changes in the sequence of their Type II ATPase sodium pumps. We show that Ophryocystis completely lacks Type II or Type 4 sodium pumps, and related proteins PMCA calcium pumps show extreme sequence divergence compared to other Apicomplexa, demonstrating new avenues of research opened by genome sequencing of non-model Apicomplexa.
(© 2023. The Author(s).)
References: Trends Parasitol. 2009 Aug;25(8):375-82. (PMID: 19635681)
Acta Trop. 2021 Jun;218:105907. (PMID: 33789154)
Mol Biol Evol. 2011 Oct;28(10):2855-71. (PMID: 21504890)
Bioinformatics. 2010 Mar 1;26(5):589-95. (PMID: 20080505)
Cells. 2020 Oct 02;9(10):. (PMID: 33023071)
Parasitology. 2007 May;134(Pt 5):657-68. (PMID: 17140464)
Bioinformatics. 2018 Mar 1;34(5):867-868. (PMID: 29096012)
Dev Cell. 2004 May;6(5):614-6. (PMID: 15130487)
Curr Biol. 2019 Dec 2;29(23):4071-4077.e3. (PMID: 31735674)
Evolution. 2016 Aug;70(8):1913-21. (PMID: 27405795)
J Anim Ecol. 2008 Jan;77(1):120-6. (PMID: 18177332)
Biochim Biophys Acta. 1968 Mar 25;151(3):655-63. (PMID: 4230809)
Proc Natl Acad Sci U S A. 2008 May 27;105(21):7489-94. (PMID: 18492806)
Proc Natl Acad Sci U S A. 2021 Dec 28;118(52):. (PMID: 34862323)
Genome Biol Evol. 2021 Feb 3;13(2):. (PMID: 33566096)
PLoS Biol. 2020 Feb 27;18(2):e3000610. (PMID: 32108180)
Int J Parasitol. 2015 Apr;45(5):285-8. (PMID: 25724591)
Mol Ecol Resour. 2021 Jul;21(5):1416-1421. (PMID: 33629477)
Mol Biol Evol. 2006 Feb;23(2):254-67. (PMID: 16221896)
Ecol Lett. 2011 May;14(5):453-61. (PMID: 21375682)
mBio. 2015 Feb 10;6(1):. (PMID: 25670772)
New Phytol. 2012 Apr;194(1):28-45. (PMID: 22292897)
Mol Biol Evol. 2021 Sep 27;38(10):4647-4654. (PMID: 34320186)
J Biol Chem. 2001 Apr 6;276(14):10782-7. (PMID: 11145964)
Nature. 2014 Oct 16;514(7522):317-21. (PMID: 25274300)
J Biol Chem. 2019 Apr 5;294(14):5720-5734. (PMID: 30723156)
Syst Biol. 2007 Apr;56(2):232-51. (PMID: 17464880)
Ecol Lett. 2010 Dec;13(12):1485-93. (PMID: 21040353)
Ann N Y Acad Sci. 1985;443:171-88. (PMID: 3860070)
J Invertebr Pathol. 2016 Oct;140:75-82. (PMID: 27642090)
Nat Methods. 2012 Mar 04;9(4):357-9. (PMID: 22388286)
J Parasitol. 1986 Jun;72(3):372-82. (PMID: 3091802)
Genome Res. 2008 Dec;18(12):1979-90. (PMID: 18757608)
J Comput Biol. 2012 May;19(5):455-77. (PMID: 22506599)
J Physiol. 1965 May;178:290-304. (PMID: 14298120)
Parasitol Today. 1999 Apr;15(4):153-6. (PMID: 10322337)
Bioinformatics. 2013 Apr 15;29(8):1072-5. (PMID: 23422339)
Proc Biol Sci. 2012 Mar 22;279(1731):1093-9. (PMID: 21920981)
Mol Ecol. 2012 Jan;21(2):340-9. (PMID: 22126595)
Genome Res. 2004 Sep;14(9):1686-95. (PMID: 15342554)
BMC Genomics. 2022 Jul 2;23(1):485. (PMID: 35780080)
G3 (Bethesda). 2022 Mar 4;12(3):. (PMID: 35100331)
J Parasitol. 2005 Dec;91(6):1287-92. (PMID: 16539007)
Curr Opin Genet Dev. 2005 Dec;15(6):601-8. (PMID: 16188433)
Genome Biol. 2013 Apr 25;14(4):R36. (PMID: 23618408)
Bioinformatics. 2011 Feb 15;27(4):592-3. (PMID: 21169378)
mBio. 2012 Sep 04;3(5):e00204-12. (PMID: 22951932)
Curr Biol. 2021 Nov 22;31(22):R1465-R1466. (PMID: 34813747)
Bioinformatics. 2009 Aug 15;25(16):2078-9. (PMID: 19505943)
Bioinformatics. 2016 Mar 1;32(5):767-9. (PMID: 26559507)
Bioinformatics. 2018 Sep 15;34(18):3094-3100. (PMID: 29750242)
Methods Mol Biol. 2019;1962:29-51. (PMID: 31020553)
J Biol Chem. 1985 Sep 25;260(21):11396-405. (PMID: 2995332)
Plant Genome. 2016 Nov;9(3):. (PMID: 27902792)
J Invertebr Pathol. 2020 Feb;170:107328. (PMID: 31952966)
BMC Bioinformatics. 2009 Dec 15;10:421. (PMID: 20003500)
معلومات مُعتمدة: ABI 1661454 National Science Foundation
فهرسة مساهمة: Keywords: ATPase; Apicomplexan genomics; Cryptic species; Monarch butterfly parasite; OE; Protist genomics; Sodium potassium pump
المشرفين على المادة: EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
9NEZ333N27 (Sodium)
تواريخ الأحداث: Date Created: 20230524 Date Completed: 20230526 Latest Revision: 20230527
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10210345
DOI: 10.1186/s12864-023-09350-0
PMID: 37226080
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2164
DOI:10.1186/s12864-023-09350-0