دورية أكاديمية
A unique effector secreted by Pseudozyma flocculosa mediates its biocontrol activity.
| العنوان: | A unique effector secreted by Pseudozyma flocculosa mediates its biocontrol activity. |
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| المؤلفون: | Santhanam P; Département de Phytologie, Université Laval, Québec, QC, Canada.; Present Address: Agriculture Agri-Food Canada, Morden, MB, Canada., Madina MH; Département de Phytologie, Université Laval, Québec, QC, Canada., Albuini FM; Département de Phytologie, Université Laval, Québec, QC, Canada.; Departamento de Bioquímica E Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Labbé C; Département de Phytologie, Université Laval, Québec, QC, Canada., Fietto LG; Departamento de Bioquímica E Biologia Molecular, Universidade Federal de Viçosa, Viçosa, MG, Brazil., Bélanger RR; Département de Phytologie, Université Laval, Québec, QC, Canada. richard.belanger@fsaa.ulaval.ca. |
| المصدر: | BMC biology [BMC Biol] 2023 May 24; Vol. 21 (1), pp. 118. Date of Electronic Publication: 2023 May 24. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: BioMed Central Country of Publication: England NLM ID: 101190720 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7007 (Electronic) Linking ISSN: 17417007 NLM ISO Abbreviation: BMC Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : BioMed Central, c2003- |
| مواضيع طبية MeSH: | Tandem Mass Spectrometry* , Basidiomycota*, Chromatography, Liquid ; Antibiosis |
| مستخلص: | Background: Pseudozyma flocculosa is a highly efficient biocontrol agent (BCA) of powdery mildews whose mode of action remains elusive. It is known to secrete unique effectors during its interaction with powdery mildews but effectors have never been shown to be part of the arsenal of a BCA. Here, we characterize the role of the effector Pf2826 released by Pseudozyma flocculosa during its tripartite interaction with barley and the pathogen fungus Blumeria graminis f. sp. hordei. Results: We utilized CRISPR-Cas9-based genome editing and confirmed that secreted P. flocculosa effector Pf2826 is required for full biocontrol activity. We monitored the localization of the effector Pf2826 with C-terminal mCherry tag and found it localized around the haustoria and on powdery mildew spores. His-tagged Pf2826 recombinant protein was expressed, purified, and used as bait in a pull-down assay from total proteins extracted during the tripartite interaction. Potential interactors were identified by LC-MS/MS analysis after removing unspecific interactions found in the negative controls. A two-way yeast two-hybrid assay validated that Pf2826 interacted with barley pathogenesis-related (PR) proteins HvPR1a and chitinase and with an effector protein from powdery mildew. Conclusions: In contrast to the usual modes of action of competition, parasitism, and antibiosis ascribed to BCAs, this study shows that effector pf2826 plays a vital role in the biocontrol activity of P. flocculosa by interacting with plant PR proteins and a powdery mildew effector, altering the host-pathogen interaction. (© 2023. The Author(s).) |
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| فهرسة مساهمة: | Keywords: Biocontrol agent; CRISPR-Cas9; Chitinase; Effectors; Fungal-fungal interactions; Hyperbiotrophy; PR proteins; Powdery mildew; Pull-down; Ustilago maydis |
| SCR Organism: | Anthracocystis flocculosa |
| تواريخ الأحداث: | Date Created: 20230524 Date Completed: 20230526 Latest Revision: 20230528 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10210494 |
| DOI: | 10.1186/s12915-023-01624-z |
| PMID: | 37226185 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1741-7007 |
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| DOI: | 10.1186/s12915-023-01624-z |