دورية أكاديمية
Xeno-Free Biomimetic ECM Model for Investigation of Matrix Composition and Stiffness on Astrocyte Cell Response.
| العنوان: | Xeno-Free Biomimetic ECM Model for Investigation of Matrix Composition and Stiffness on Astrocyte Cell Response. |
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| المؤلفون: | Saleh BM; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA., Pourmostafa A; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA., Patrawalla NY; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA., Kishore V; Department of Biomedical and Chemical Engineering and Sciences, Florida Institute of Technology, Melbourne, FL 32901, USA. |
| المصدر: | Journal of functional biomaterials [J Funct Biomater] 2023 May 05; Vol. 14 (5). Date of Electronic Publication: 2023 May 05. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101570734 Publication Model: Electronic Cited Medium: Print ISSN: 2079-4983 (Print) Linking ISSN: 20794983 NLM ISO Abbreviation: J Funct Biomater Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Basel : MDPI AG |
| مستخلص: | Astrocytes, highly specialized glial cells, play a critical role in neuronal function. Variations in brain extracellular matrix (ECM) during development and disease can significantly alter astrocyte cell function. Age-related changes in ECM properties have been linked to neurodegenerative diseases such as Alzheimer's disease. The goal of this study was to develop hydrogel-based biomimetic ECM models with varying stiffness and evaluate the effects of ECM composition and stiffness on astrocyte cell response. Xeno-free ECM models were synthesized by combining varying ratios of human collagen and thiolated hyaluronic acid (HA) crosslinked with polyethylene glycol diacrylate. Results showed that modulating ECM composition yielded hydrogels with varying stiffnesses that match the stiffness of the native brain ECM. Collagen-rich hydrogels swell more and exhibit greater stability. Higher metabolic activity and greater cell spreading was observed in hydrogels with lower HA. Soft hydrogels trigger astrocyte activation indicated by greater cell spreading, high GFAP expression and low ALDH1L1 expression. This work presents a baseline ECM model to investigate the synergistic effects of ECM composition and stiffness on astrocytes, which could be further developed to identify key ECM biomarkers and formulate new therapies to alleviate the impact of ECM changes on the onset and progression of neurodegenerative diseases. |
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| فهرسة مساهمة: | Keywords: Alzheimer’s disease; astrocytes; collagen; extracellular matrix; hyaluronic acid; hydrogel |
| تواريخ الأحداث: | Date Created: 20230526 Latest Revision: 20230529 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10218816 |
| DOI: | 10.3390/jfb14050256 |
| PMID: | 37233366 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2079-4983 |
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| DOI: | 10.3390/jfb14050256 |