دورية أكاديمية
أعرض في EDS

Rapid and Facile Light-Based Approach to Fabricate Protease-Degradable Poly(ethylene glycol)-norbornene Microgels for Cell Encapsulation.

التفاصيل البيبلوغرافية
العنوان: Rapid and Facile Light-Based Approach to Fabricate Protease-Degradable Poly(ethylene glycol)-norbornene Microgels for Cell Encapsulation.
المؤلفون: Mora-Boza A; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA.; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA.; CÚRAM, University of Galway, Galway, H91 W2TY, Ireland., Ghebrezadik SG; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA.; Agnes Scott College, Decatur, GA, 30030, USA., Leisen JE; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA., García AJ; Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA.; Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA, 30332-0363, USA.
المصدر: Advanced healthcare materials [Adv Healthc Mater] 2023 Oct; Vol. 12 (26), pp. e2300942. Date of Electronic Publication: 2023 Jun 01.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 101581613 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2192-2659 (Electronic) Linking ISSN: 21922640 NLM ISO Abbreviation: Adv Healthc Mater Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Weinheim : Wiley-VCH, 2012-
مواضيع طبية MeSH: Polyethylene Glycols* , Microgels*, Humans ; Cell Encapsulation ; Peptide Hydrolases ; Hydrogels ; Biocompatible Materials ; Norbornanes
مستخلص: Thiol-norbornene photoclickable poly (ethylene glycol) (PEG)-based (PEG-NB) hydrogels are attractive biomaterials for cell encapsulation, drug delivery, and regenerative medicine applications. Although many crosslinking strategies and chemistries have been developed for PEG-NB bulk hydrogels, fabrication approaches of PEG-NB microgels have not been extensively explored. Here, a fabrication strategy for 4-arm amide-linked PEG-NB (PEG-4aNB) microgels using flow-focusing microfluidics for human mesenchymal stem/stromal cell (hMSCs) encapsulation is presented. PEG-4aNB photochemistry allows high-throughput, ultrafast generation, and cost-effective synthesis of monodispersed microgels (diameter 340 ± 18, 380 ± 24, and 420 ± 15 µm, for 6, 8, and 10 wt% of PEG-4aNB, respectively) using an in situ crosslinking methodology in a microfluidic device. PEG-4aNB microgels show in vitro degradability due to the incorporation of a protease-degradable peptide during photocrosslinking and encapsulated cells show excellent viability and metabolic activity for at least 13 days of culture. Furthermore, the secretory profile (i.e., MMP-13, ICAM-1, PD-L1, CXCL9, CCL3/MIP-1, IL-6, IL-12, IL-17E, TNF-α, CCL2/MCP-1) of encapsulated hMSCs shows increased expression in response to IFN-γ stimulation. Collectively, this work shows a versatile and facile approach for the fabrication of protease-degradable PEG-4aNB microgels for cell encapsulation.
(© 2023 Wiley-VCH GmbH.)
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معلومات مُعتمدة: P40 OD011050 United States OD NIH HHS; R01 AR062368 United States AR NIAMS NIH HHS
فهرسة مساهمة: Keywords: mesenchymal stem/stromal cells; microfluidics; microgels; photopolymerization; poly(ethylene glycol)−norbornene
المشرفين على المادة: 3WJQ0SDW1A (Polyethylene Glycols)
0 (Microgels)
EC 3.4.- (Peptide Hydrolases)
0 (Hydrogels)
0 (Biocompatible Materials)
0 (Norbornanes)
تواريخ الأحداث: Date Created: 20230526 Date Completed: 20231023 Latest Revision: 20231025
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10592588
DOI: 10.1002/adhm.202300942
PMID: 37235850
قاعدة البيانات: MEDLINE
الوصف
تدمد:2192-2659
DOI:10.1002/adhm.202300942