UPLC-QE-Orbitrap-Based Cell Metabolomics and Network Pharmacology to Reveal the Mechanism of N-Benzylhexadecanamide Isolated from Maca ( Lepidium meyenii Walp.) against Testicular Dysfunction.

التفاصيل البيبلوغرافية
العنوان:	UPLC-QE-Orbitrap-Based Cell Metabolomics and Network Pharmacology to Reveal the Mechanism of N-Benzylhexadecanamide Isolated from Maca ( Lepidium meyenii Walp.) against Testicular Dysfunction.
المؤلفون:	Zhang KY; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Li CN; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Zhang NX; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Gao XC; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Shen JM; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Cheng DD; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Wang YL; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Zhang H; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Lv JW; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China., Sun JM; Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, China.
المصدر:	Molecules (Basel, Switzerland) [Molecules] 2023 May 12; Vol. 28 (10). Date of Electronic Publication: 2023 May 12.
نوع المنشور:	Journal Article
اللغة:	English
بيانات الدورية:	Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة:	Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH:	Tandem Mass Spectrometry* , Lepidium*/chemistry, Mice ; Male ; Animals ; Network Pharmacology ; Hydrogen Peroxide ; Polyunsaturated Alkamides ; Testosterone ; Metabolomics
مستخلص:	Testicular dysfunction (TDF) is characterized by testosterone deficiency and is caused by oxidative stress injury in Leydig cells. A natural fatty amide named N-benzylhexadecanamide (NBH), derived from cruciferous maca, has been shown to promote testosterone production. Our study aims to reveal the anti-TDF effect of NBH and explore its potential mechanism in vitro. This study examined the effects of H 2 O 2 on cell viability and testosterone levels in mouse Leydig cells (TM3) under oxidative stress. In addition, cell metabolomics analysis based on UPLC-Q-Exactive-MS/MS showed that NBH was mainly involved in arginine biosynthesis, aminoacyl-tRNA biosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, the TCA cycle and other metabolic pathways by affecting 23 differential metabolites, including arginine and phenylalanine. Furthermore, we also performed network pharmacological analysis to observe the key protein targets in NBH treatment. The results showed that its role was to up-regulate ALOX5, down-regulate CYP1A2, and play a role in promoting testicular activity by participating in the steroid hormone biosynthesis pathway. In summary, our study not only provides new insights into the biochemical mechanisms of natural compounds in the treatment of TDF, but also provides a research strategy that integrates cell metabolomics and network pharmacology in order to promote the screening of new drugs for the treatment of TDF.
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معلومات مُعتمدة:	31570347 National Natural Science Foundation of China; 2022011 Jilin Province Development and Reform Commission; 20200404081YY Jilin Province Science and Technology Department
فهرسة مساهمة:	Keywords: N-benzylhexadecanamide; mechanisms; metabolomics; network pharmacology; testicular dysfunction
المشرفين على المادة:	1M5UVS8JUU (N-benzylhexadecanamide) BBX060AN9V (Hydrogen Peroxide) 0 (Polyunsaturated Alkamides) 3XMK78S47O (Testosterone)
تواريخ الأحداث:	Date Created: 20230527 Date Completed: 20230529 Latest Revision: 20230530
رمز التحديث:	20231215
مُعرف محوري في PubMed:	PMC10222419
DOI:	10.3390/molecules28104064
PMID:	37241805
قاعدة البيانات:	MEDLINE

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تدمد:	1420-3049
DOI:	10.3390/molecules28104064