دورية أكاديمية
أعرض في EDS

Chitosan, Chitosan/IgG-Loaded, and N-Trimethyl Chitosan Chloride Nanoparticles as Potential Adjuvant and Carrier-Delivery Systems.

التفاصيل البيبلوغرافية
العنوان: Chitosan, Chitosan/IgG-Loaded, and N-Trimethyl Chitosan Chloride Nanoparticles as Potential Adjuvant and Carrier-Delivery Systems.
المؤلفون: Tenorio-Barajas AY; Doctorado en Nanociencias y Nanotecnología, Centro de Investigación y Estudios Avanzados del IPN, Ciudad de México 07360, Mexico.; Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico., Olvera ML; Sección de Estado Sólido, Departamento de Ingeniería Eléctrica, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México 07360, Mexico., Romero-Paredes G; Sección de Estado Sólido, Departamento de Ingeniería Eléctrica, Centro de Investigación y de Estudios Avanzados del IPN, Ciudad de México 07360, Mexico., Altuzar V; Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico., Garrido-Guerrero E; Departamento de Genética y Biología Celular, Centro de Investigación y Estudios Avanzados del IPN, Ciudad de México 07360, Mexico., Mendoza-Barrera C; Facultad de Ciencias Físico Matemáticas, Benemérita Universidad Autónoma de Puebla, Puebla 72570, Mexico.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2023 May 15; Vol. 28 (10). Date of Electronic Publication: 2023 May 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Chitosan* , Nanoparticles*, Humans ; Drug Carriers ; Immunoglobulin G ; Particle Size
مستخلص: This work proposes a feasible, reproducible, and low-cost modified method to manufacture chitosan, chitosan/IgG-protein-loaded, and trimethylated chitosan nanoparticles, using microfluidics combined with the microemulsion technique, which differs from the traditional batch process of chitosan-based nanoparticles. The synthesis process consists of generating microreactors of chitosan-based polymer in a poly-dimethylsiloxane ψ-shaped microfluidic device and then crosslinking with sodium tripolyphosphate outside the cell. Transmission electron microscopy demonstrates an improvement in size control and distribution of the solid-shape chitosan nanoparticles (~80 nm) compared to the batch synthesis. Regarding chitosan/IgG-protein-loaded nanoparticles, these presented a core-shell morphology having a diameter of close to 15 nm. Raman and X-ray photoelectron spectroscopies confirmed the ionic crosslinking between the amino groups of chitosan and the phosphate groups of sodium tripolyphosphate in the fabricated samples and the total encapsulation of IgG protein during the fabrication of chitosan/IgG-loaded nanoparticles. Then, an ionic crosslinking and nucleation-diffusion process of chitosan-sodium tripolyphosphate was carried out during the nanoparticle formation, with and without IgG protein loading. The use of N-trimethyl chloride chitosan nanoparticles in vitro on human-keratinocyte-derived cell line HaCaT did not show side effects independently of its concentration from 1 to 10 μg/mL. Therefore, the proposed materials could be used as potential carrier-delivery systems.
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معلومات مُعتمدة: 286652 and A1-S-31186 Consejo Nacional de Ciencia y Tecnología
فهرسة مساهمة: Keywords: chitosan; in vitro culture; loaded nanoparticles; microfluidics
المشرفين على المادة: 9012-76-4 (Chitosan)
0 (Drug Carriers)
0 (Immunoglobulin G)
0 (N-trimethyl chitosan chloride)
NU43IAG5BC (triphosphoric acid)
تواريخ الأحداث: Date Created: 20230527 Date Completed: 20231011 Latest Revision: 20231011
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10221049
DOI: 10.3390/molecules28104107
PMID: 37241848
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules28104107