دورية أكاديمية
[Discussion on two common problems in renal transplantation donor-specificity discrimination of anti-human leukocyte antigen antibody].
| العنوان: | [Discussion on two common problems in renal transplantation donor-specificity discrimination of anti-human leukocyte antigen antibody]. |
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| المؤلفون: | Hao MJ; Department of Organ Transplantation, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200433, China., Ma LP; Suzhou Caibo Medical Research Institute, Suzhou 215000, China., Chen HJ; Department of Organ Transplantation, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200433, China., Li YH; Department of Nursing, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200433, China., Zhang L; Department of Organ Transplantation, Changhai Hospital Affiliated to Naval Medical University, Shanghai 200433, China. |
| المصدر: | Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 2023 May 30; Vol. 103 (20), pp. 1571-1574. |
| نوع المنشور: | English Abstract; Journal Article |
| اللغة: | Chinese |
| بيانات الدورية: | Publisher: Zhonghua yi xue hui Country of Publication: China NLM ID: 7511141 Publication Model: Print Cited Medium: Print ISSN: 0376-2491 (Print) Linking ISSN: 03762491 NLM ISO Abbreviation: Zhonghua Yi Xue Za Zhi Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Beijing : Zhonghua yi xue hui Original Publication: Beijing : Zhonghua yi xue hui. |
| مواضيع طبية MeSH: | Kidney Transplantation*, Graft Rejection/diagnosis ; Graft Rejection/etiology ; Isoantibodies ; Tissue Donors ; Humans |
| مستخلص: | Antibody-mediated rejection (AMR) is a key factor affecting the long-term survival of renal allografts. Donor-specific antibody (DSA) is the etiology of AMR. So it is very important to accurately detect DSA. The single antigen bead (SAB) method, which is widely used in clinical practice, is prone to miss DSA detection and underestimate its mean fluorescence intensity (MFI). In this paper, the probability of missed detection of two SAB reagents was calculated by comparing common HLA alleles in China population, and the in vitro effect of antibody cross reaction on MFI value of DSA was revealed. The authors emphasized the clinical significance of the above two problems, tried to manage them by using functional epitope (eplet) analysis and give some clinical examples. Finally, the limitations of this correction method were analyzed. |
| فهرسة مساهمة: | Local Abstract: [Publisher, Chinese] 抗体介导排斥反应（AMR）是影响移植肾长期存活的关键因素，供者特异性抗体（DSA）是AMR的病因，其准确判定至关重要。目前临床广泛应用的单抗原微珠法（SAB）判别DSA时容易出现漏检以及平均荧光强度（MFI）值低估两个问题。本文通过与中国人群常见人类白细胞抗原（HLA）等位基因进行比对，计算了常用的两种SAB试剂的漏检概率，探讨了体外实验体系中抗体交叉反应对DSA的MFI值的影响。笔者提出应重视上述两个问题的临床意义，尝试用功能性表位（eplet）分析的方法予以校正并列举了临床实例，最后分析了这种校正方法的局限性。. |
| المشرفين على المادة: | 0 (Isoantibodies) |
| تواريخ الأحداث: | Date Created: 20230528 Date Completed: 20230530 Latest Revision: 20230530 |
| رمز التحديث: | 20231215 |
| DOI: | 10.3760/cma.j.cn112137-20221228-02711 |
| PMID: | 37246009 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 0376-2491 |
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| DOI: | 10.3760/cma.j.cn112137-20221228-02711 |