التفاصيل البيبلوغرافية
| العنوان: |
High-Speed Magnetic Tweezers for Nanomechanical Measurements on Force-Sensitive Elements. |
| المؤلفون: |
Park C; Department of Physics, Pohang University of Science and Technology (POSTECH)., Yang T; Department of Physics, Pohang University of Science and Technology (POSTECH)., Rah SH; Department of Physics, Pohang University of Science and Technology (POSTECH)., Kim HG; School of Biological Sciences, Seoul National University; Institute for Molecular Biology and Genetics, Seoul National University., Yoon TY; School of Biological Sciences, Seoul National University; Institute for Molecular Biology and Genetics, Seoul National University; tyyoon@snu.ac.kr., Shon MJ; Department of Physics, Pohang University of Science and Technology (POSTECH); School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH); mjshon@postech.ac.kr. |
| المصدر: |
Journal of visualized experiments : JoVE [J Vis Exp] 2023 May 12 (195). Date of Electronic Publication: 2023 May 12. |
| نوع المنشور: |
Journal Article; Video-Audio Media; Research Support, Non-U.S. Gov't |
| اللغة: |
English |
| بيانات الدورية: |
Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: |
Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: |
Magnetics*/methods , Mechanical Phenomena*, DNA/chemistry ; Nanotechnology ; Magnetic Fields ; Optical Tweezers |
| مستخلص: |
Single-molecule magnetic tweezers (MTs) have served as powerful tools to forcefully interrogate biomolecules, such as nucleic acids and proteins, and are therefore poised to be useful in the field of mechanobiology. Since the method commonly relies on image-based tracking of magnetic beads, the speed limit in recording and analyzing images, as well as the thermal fluctuations of the beads, has long hampered its application in observing small and fast structural changes in target molecules. This article describes detailed methods for the construction and operation of a high-resolution MT setup that can resolve nanoscale, millisecond dynamics of biomolecules and their complexes. As application examples, experiments with DNA hairpins and SNARE complexes (membrane-fusion machinery) are demonstrated, focusing on how their transient states and transitions can be detected in the presence of piconewton-scale forces. We expect that high-speed MTs will continue to enable high-precision nanomechanical measurements on molecules that sense, transmit, and generate forces in cells, and thereby deepen our molecular-level understanding of mechanobiology. |
| المشرفين على المادة: |
9007-49-2 (DNA) |
| تواريخ الأحداث: |
Date Created: 20230529 Date Completed: 20230531 Latest Revision: 20230531 |
| رمز التحديث: |
20230531 |
| DOI: |
10.3791/65137 |
| PMID: |
37246853 |
| قاعدة البيانات: |
MEDLINE |
