دورية أكاديمية
Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma.
| العنوان: | Overexpression of heat-shock protein 47 impacts survival of patients with oral squamous cell carcinoma. |
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| المؤلفون: | da Costa BC; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Dourado MR; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., de Moraes EF; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Panini LM; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Elseragy A; Research Unit of Population Health, University of Oulu, Medical Research Centre and Oulu University Hospital, Oulu, Finland., Téo FH; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Guimarães GN; Department of Biosciences and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Machado RA; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Risteli M; Research Unit of Population Health, University of Oulu, Medical Research Centre and Oulu University Hospital, Oulu, Finland., Gurgel Rocha CA; Gonçalo Moniz Institute, Oswaldo Cruz Foundation, Federal University of Bahia and Dor Institute, Salvador, Bahia, Brazil., Paranaíba LMR; Department of Pathology and Parasitology, Institute of Biomedical Sciences, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil., González-Arriagada WA; Facultad de Odontología and Centro de Investigación e Innovación Biomédica (CIIB), Universidad de los Andes, Santiago, Chile., da Silva SD; Lady Davis Institute for Medical Research and Segal Cancer Center, Jewish General Hospital, and Department of Otolaryngology-Head and Neck Surgery, McGill University, Montreal, Quebec, Canada., Rangel ALCA; Department of Oral Pathology and Oral Medicine, Dental School, Western Paraná State University, Cascavel, Paraná, Brazil., Marques MR; Department of Biosciences and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil., Salo T; Research Unit of Population Health, University of Oulu, Medical Research Centre and Oulu University Hospital, Oulu, Finland.; Department of Oral and Maxillofacial Diseases and Department of Pathology, Helsinki University Central Hospital, and Translational Immunology Research Program, University of Helsinki, Helsinki, Finland., Coletta RD; Department of Oral Diagnosis and Graduate Program in Oral Biology, Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil. |
| المصدر: | Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology [J Oral Pathol Med] 2023 Aug; Vol. 52 (7), pp. 601-609. Date of Electronic Publication: 2023 May 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: Denmark NLM ID: 8911934 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0714 (Electronic) Linking ISSN: 09042512 NLM ISO Abbreviation: J Oral Pathol Med Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Oxford, UK : Wiley-Blackwell Original Publication: Copenhagen : Munksgaard, c1989- |
| مواضيع طبية MeSH: | Carcinoma, Squamous Cell*/pathology , Mouth Neoplasms*/pathology , Head and Neck Neoplasms*, Humans ; Squamous Cell Carcinoma of Head and Neck ; HSP47 Heat-Shock Proteins/genetics ; HSP47 Heat-Shock Proteins/metabolism ; Cisplatin/pharmacology ; Cell Line, Tumor ; Cell Proliferation/genetics ; Cell Movement/genetics |
| مستخلص: | Background: The expression of heat-shock protein 47 (HSP47) has been linked to collagen synthesis control and implicated in fibrotic disorders, but more recent studies have demonstrated its role in solid tumors. In this study, we explored the prognostic impact of HSP47 in oral squamous cell carcinomas (OSCC) and determined the in vitro effects of its loss-of-function on viability, proliferation, migration, invasion, and resistance to cisplatin of OSCC cells. Methods: The HSP47 expression in tumor samples was assessed by immunohistochemistry in two independent cohorts totaling 339 patients with OSCC, and protein levels were associated with clinicopathological features and survival outcomes. The OSCC cell lines HSC3 and SCC9 were transduced with lentivirus expressing short hairpin RNA to stably silence HSP47 and used in assays to measure cellular viability, proliferation, migration, and invasion. Results: HSP47 was overexpressed in OSCC samples, and its overexpression was significantly and independently associated with poor disease-specific survival and shortened disease-free survival in both OSCC cohorts. The knockdown of HSP47 showed no effects on cell viability or cisplatin sensitivity, but impaired significantly proliferation, migration, and invasion of OSCC cells, with stronger effects on SCC9 cells. Conclusion: Our results show a significant prognostic impact of HSP47 overexpression in OSCC and reveal that HSP47 inhibition impairs the proliferation, migration, and invasion of OSCC cells. HSP47 may represent a potential therapeutic target for OSCC. (© 2023 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.) |
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| معلومات مُعتمدة: | 407814/2018-3 Conselho Nacional de Desenvolvimento Científico e Tecnológico; PPM-00179-18 Fundação de Amparo à Pesquisa do Estado de Minas Gerais; 2018/16077-6 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2021/08943-8 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2022/00994-5 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2022/01123-8 Fundação de Amparo à Pesquisa do Estado de São Paulo; 2021/13595-9 Fundação de Amparo à Pesquisa do Estado de São Paulo; Universities of Oulu and Helsinki; Sigrid Juselius Foundation; Oulu and Helsinki University Hospital special state support for research |
| فهرسة مساهمة: | Keywords: SERPINH1; invasion; oral cancer; prognosis; proliferation |
| المشرفين على المادة: | 0 (HSP47 Heat-Shock Proteins) Q20Q21Q62J (Cisplatin) |
| تواريخ الأحداث: | Date Created: 20230529 Date Completed: 20230808 Latest Revision: 20230808 |
| رمز التحديث: | 20230808 |
| DOI: | 10.1111/jop.13455 |
| PMID: | 37247331 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1600-0714 |
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| DOI: | 10.1111/jop.13455 |