دورية أكاديمية
أعرض في EDS

Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components.

التفاصيل البيبلوغرافية
العنوان: Single-cell proteo-genomic reveals a comprehensive map of centrosome-associated spliceosome components.
المؤلفون: Cerulo L; Bioinformatics Laboratory, BIOGEM scrl, Ariano Irpino, Avellino, Italy.; Department of Sciences and Technologies, University of Sannio, Benevento, Italy., Pezzella N; Telethon Institute of Genetics and Medicine (TIGEM), Via Campi Flegrei, 34, Pozzuoli, 80078 Naples, Italy.; School for Advanced Studies, Genomics and Experimental Medicine Program, Naples, Italy., Caruso FP; Bioinformatics Laboratory, BIOGEM scrl, Ariano Irpino, Avellino, Italy.; Department of Sciences and Technologies, University of Sannio, Benevento, Italy., Parente P; Unit of Pathology, Fondazione IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Foggia, Italy., Remo A; Pathology Unit, Mater Salutis Hospital AULSS9, 'Scaligera', 37122 Verona, Italy., Giordano G; Unit of Medical Oncology and Biomolecular Therapy, Department of Medical and Surgical Sciences, University of Foggia, Policlinico Riuniti, 71122 Foggia, Italy., Forte N; Department of Clinical Pathology, Fatebenefratelli Hospital, 82100 Benevento, Italy., Busselez J; Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France., Boschi F; Department of Computer Science, University of Verona, Strada Le Grazie 8, Verona, Italy., Galiè M; Department of Neuroscience, Biomedicine and Movement, University of Verona, Verona, Italy., Franco B; Telethon Institute of Genetics and Medicine (TIGEM), Via Campi Flegrei, 34, Pozzuoli, 80078 Naples, Italy.; School for Advanced Studies, Genomics and Experimental Medicine Program, Naples, Italy.; Medical Genetics, Department of Translational Medicine, University of Naples 'Federico II', Via Sergio Pansini, 80131 Naples, Italy., Pancione M; Department of Sciences and Technologies, University of Sannio, Benevento, Italy.; Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Complutense University Madrid, 28040 Madrid, Spain.
المصدر: IScience [iScience] 2023 Apr 10; Vol. 26 (5), pp. 106602. Date of Electronic Publication: 2023 Apr 10 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Cell Press Country of Publication: United States NLM ID: 101724038 Publication Model: eCollection Cited Medium: Internet ISSN: 2589-0042 (Electronic) Linking ISSN: 25890042 NLM ISO Abbreviation: iScience Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: [Cambridge, MA] : Cell Press, [2018]-
مستخلص: Ribonucleoprotein (RNP) condensates are crucial for controlling RNA metabolism and splicing events in animal cells. We used spatial proteomics and transcriptomic to elucidate RNP interaction networks at the centrosome, the main microtubule-organizing center in animal cells. We found a number of cell-type specific centrosome-associated spliceosome interactions localized in subcellular structures involved in nuclear division and ciliogenesis. A component of the nuclear spliceosome BUD31 was validated as an interactor of the centriolar satellite protein OFD1. Analysis of normal and disease cohorts identified the cholangiocarcinoma as target of centrosome-associated spliceosome alterations. Multiplexed single-cell fluorescent microscopy for the centriole linker CEP250 and spliceosome components including BCAS2, BUD31, SRSF2 and DHX35 recapitulated bioinformatic predictions on the centrosome-associated spliceosome components tissue-type specific composition. Collectively, centrosomes and cilia act as anchor for cell-type specific spliceosome components, and provide a helpful reference for explore cytoplasmic condensates functions in defining cell identity and in the origin of rare diseases.
Competing Interests: The authors declare no competing interests.
(© 2023 The Author(s).)
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فهرسة مساهمة: Keywords: Biochemistry; Cell biology; Genomics; Proteomics
تواريخ الأحداث: Date Created: 20230530 Latest Revision: 20230531
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10214398
DOI: 10.1016/j.isci.2023.106602
PMID: 37250316
قاعدة البيانات: MEDLINE
الوصف
تدمد:2589-0042
DOI:10.1016/j.isci.2023.106602