دورية أكاديمية
Microfibril-associated protein 5 and the regulation of skin scar formation.
| العنوان: | Microfibril-associated protein 5 and the regulation of skin scar formation. |
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| المؤلفون: | Han C; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA., Leonardo TR; Department of Microbiology and Immunology, College of Medicine, University of Illinois Chicago, Chicago, IL, USA., Romana-Souza B; Department of Histology and Embryology, Rio de Janeiro State University, Rio de Janeiro, RJ, Brazil., Shi J; NMPA Key Laboratory for Clinical Research and Evaluation of Traditional Chinese Medicine, Xiyuan Hospital, China Academy of Chinese Medical Sciences, Beijing, China., Keiser S; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA., Yuan H; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA., Altakriti M; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA., Ranzer MJ; Department of Surgery, University of Illinois Chicago, Chicago, IL, USA., Ferri-Borgogno S; Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center, Houston, TX, USA., Mok SC; Department of Gynecologic Oncology and Reproductive Medicine, University of Texas MD Anderson Cancer Center, Houston, TX, USA., Koh TJ; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA.; Department of Kinesiology and Nutrition, University of Illinois Chicago, Chicago, IL, USA., Hong SJ; Department of Surgery, Northwestern University-Feinberg School of Medicine, Chicago, IL, USA., Chen L; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA. Chenlin@uic.edu., DiPietro LA; Center for Wound Healing and Tissue Regeneration, University of Illinois Chicago, Chicago, IL, USA. Ldipiet@uic.edu. |
| المصدر: | Scientific reports [Sci Rep] 2023 May 30; Vol. 13 (1), pp. 8728. Date of Electronic Publication: 2023 May 30. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
| مواضيع طبية MeSH: | Cicatrix*/pathology , Wound Healing*/physiology , Intercellular Signaling Peptides and Proteins*/metabolism , Contractile Proteins*/metabolism, Animals ; Mice ; Fibroblasts/metabolism ; Fibrosis ; Microfibrils ; Skin/metabolism |
| مستخلص: | Many factors regulate scar formation, which yields a modified extracellular matrix (ECM). Among ECM components, microfibril-associated proteins have been minimally explored in the context of skin wound repair. Microfibril-associated protein 5 (MFAP5), a small 25 kD serine and threonine rich microfibril-associated protein, influences microfibril function and modulates major extracellular signaling pathways. Though known to be associated with fibrosis and angiogenesis in certain pathologies, MFAP5's role in wound healing is unknown. Using a murine model of skin wound repair, we found that MFAP5 is significantly expressed during the proliferative and remodeling phases of healing. Analysis of existing single-cell RNA-sequencing data from mouse skin wounds identified two fibroblast subpopulations as the main expressors of MFAP5 during wound healing. Furthermore, neutralization of MFAP5 in healing mouse wounds decreased collagen deposition and refined angiogenesis without altering wound closure. In vitro, recombinant MFAP5 significantly enhanced dermal fibroblast migration, collagen contractility, and expression of pro-fibrotic genes. Additionally, TGF-ß1 increased MFAP5 expression and production in dermal fibroblasts. Our findings suggest that MFAP5 regulates fibroblast function and influences scar formation in healing wounds. Our work demonstrates a previously undescribed role for MFAP5 and suggests that microfibril-associated proteins may be significant modulators of wound healing outcomes and scarring. (© 2023. The Author(s).) |
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| معلومات مُعتمدة: | GM50875 United States NH NIH HHS; F31 AR082287 United States AR NIAMS NIH HHS; DE028747-01 United States NH NIH HHS; AR082287-01 United States NH NIH HHS; R35 GM139603 United States GM NIGMS NIH HHS |
| المشرفين على المادة: | 0 (Intercellular Signaling Peptides and Proteins) 0 (Contractile Proteins) |
| تواريخ الأحداث: | Date Created: 20230530 Date Completed: 20230602 Latest Revision: 20240227 |
| رمز التحديث: | 20240227 |
| مُعرف محوري في PubMed: | PMC10229580 |
| DOI: | 10.1038/s41598-023-35558-x |
| PMID: | 37253753 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2045-2322 |
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| DOI: | 10.1038/s41598-023-35558-x |