دورية أكاديمية
Design principles for selective polarization of PAR proteins by cortical flows.
العنوان: | Design principles for selective polarization of PAR proteins by cortical flows. |
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المؤلفون: | Illukkumbura R; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Hirani N; The Francis Crick Institute , London, UK., Borrego-Pinto J; The Francis Crick Institute , London, UK., Bland T; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Ng K; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Hubatsch L; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., McQuade J; Department of Life Sciences, Imperial College London, London, UK., Endres RG; Department of Life Sciences, Imperial College London, London, UK., Goehring NW; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK. |
المصدر: | The Journal of cell biology [J Cell Biol] 2023 Aug 07; Vol. 222 (8). Date of Electronic Publication: 2023 Jun 02. |
نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
اللغة: | English |
بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
مواضيع طبية MeSH: | Actins*/metabolism , Caenorhabditis elegans Proteins*/metabolism , Protein Serine-Threonine Kinases*/metabolism, Animals ; Actomyosin/metabolism ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Cell Polarity ; Cytoplasm/metabolism ; Embryo, Nonmammalian/metabolism |
مستخلص: | Clustering of membrane-associated molecules is thought to promote interactions with the actomyosin cortex, enabling size-dependent transport by actin flows. Consistent with this model, in the Caenorhabditis elegans zygote, efficient anterior segregation of the polarity protein PAR-3 requires oligomerization. However, through direct assessment of local coupling between motion of PAR proteins and the underlying cortex, we find no links between PAR-3 oligomer size and the degree of coupling. Indeed, both anterior and posterior PAR proteins experience similar advection velocities, at least over short distances. Consequently, differential cortex engagement cannot account for selectivity of PAR protein segregation by cortical flows. Combining experiment and theory, we demonstrate that a key determinant of differential segregation of PAR proteins by cortical flow is the stability of membrane association, which is enhanced by clustering and enables transport across cellular length scales. Thus, modulation of membrane binding dynamics allows cells to achieve selective transport by cortical flows despite widespread coupling between membrane-associated molecules and the cell cortex. (© 2023 Illukkumbura et al.) |
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معلومات مُعتمدة: | P40 OD010440 United States OD NIH HHS; United Kingdom WT_ Wellcome Trust; BB/M011178/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; FC001086 United Kingdom ARC_ Arthritis Research UK; FC001086 United Kingdom WT_ Wellcome Trust; FC001086 United Kingdom MRC_ Medical Research Council; FC001086 United Kingdom CRUK_ Cancer Research UK |
المشرفين على المادة: | 0 (Actins) 9013-26-7 (Actomyosin) 0 (Caenorhabditis elegans Proteins) EC 2.7.11.1 (PAR-3 protein, C elegans) EC 2.7.11.1 (Protein Serine-Threonine Kinases) |
تواريخ الأحداث: | Date Created: 20230602 Date Completed: 20230605 Latest Revision: 20230619 |
رمز التحديث: | 20230620 |
مُعرف محوري في PubMed: | PMC10238861 |
DOI: | 10.1083/jcb.202209111 |
PMID: | 37265444 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1540-8140 |
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DOI: | 10.1083/jcb.202209111 |