دورية أكاديمية
Design principles for selective polarization of PAR proteins by cortical flows.
| العنوان: | Design principles for selective polarization of PAR proteins by cortical flows. |
|---|---|
| المؤلفون: | Illukkumbura R; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Hirani N; The Francis Crick Institute , London, UK., Borrego-Pinto J; The Francis Crick Institute , London, UK., Bland T; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Ng K; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., Hubatsch L; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK., McQuade J; Department of Life Sciences, Imperial College London, London, UK., Endres RG; Department of Life Sciences, Imperial College London, London, UK., Goehring NW; The Francis Crick Institute , London, UK.; Institute for the Physics of Living Systems, University College London , London, UK. |
| المصدر: | The Journal of cell biology [J Cell Biol] 2023 Aug 07; Vol. 222 (8). Date of Electronic Publication: 2023 Jun 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Rockefeller University Press Country of Publication: United States NLM ID: 0375356 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1540-8140 (Electronic) Linking ISSN: 00219525 NLM ISO Abbreviation: J Cell Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Rockefeller University Press |
| مواضيع طبية MeSH: | Actins*/metabolism , Caenorhabditis elegans Proteins*/metabolism , Protein Serine-Threonine Kinases*/metabolism, Animals ; Actomyosin/metabolism ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans/metabolism ; Cell Polarity ; Cytoplasm/metabolism ; Embryo, Nonmammalian/metabolism |
| مستخلص: | Clustering of membrane-associated molecules is thought to promote interactions with the actomyosin cortex, enabling size-dependent transport by actin flows. Consistent with this model, in the Caenorhabditis elegans zygote, efficient anterior segregation of the polarity protein PAR-3 requires oligomerization. However, through direct assessment of local coupling between motion of PAR proteins and the underlying cortex, we find no links between PAR-3 oligomer size and the degree of coupling. Indeed, both anterior and posterior PAR proteins experience similar advection velocities, at least over short distances. Consequently, differential cortex engagement cannot account for selectivity of PAR protein segregation by cortical flows. Combining experiment and theory, we demonstrate that a key determinant of differential segregation of PAR proteins by cortical flow is the stability of membrane association, which is enhanced by clustering and enables transport across cellular length scales. Thus, modulation of membrane binding dynamics allows cells to achieve selective transport by cortical flows despite widespread coupling between membrane-associated molecules and the cell cortex. (© 2023 Illukkumbura et al.) |
| References: | Development. 2008 Nov;135(22):3665-75. (PMID: 18842813) Dev Cell. 2018 Jul 2;46(1):9-22.e4. (PMID: 29937389) Nat New Biol. 1971 Oct 20;233(42):225-9. (PMID: 20480991) Nat Methods. 2012 Jun 28;9(7):676-82. (PMID: 22743772) Biophys J. 2019 Sep 3;117(5):791-792. (PMID: 31422823) Biophys J. 2010 Oct 20;99(8):2443-52. (PMID: 20959084) Genetics. 2014 Nov;198(3):837-46. (PMID: 25161212) Dev Cell. 2017 Aug 21;42(4):400-415.e9. (PMID: 28781174) Science. 2007 Jan 5;315(5808):111-5. (PMID: 17204653) Nat Phys. 2019 Jun 24;15(10):1075-1085. (PMID: 31579399) Bull Math Biol. 2022 Feb 10;84(3):40. (PMID: 35142872) J Cell Biol. 2009 Feb 23;184(4):473-9. (PMID: 19221192) Structure. 2013 Jun 4;21(6):997-1006. (PMID: 23643951) Nat Cell Biol. 2011 Oct 09;13(11):1361-7. (PMID: 21983565) Nat Methods. 2008 Feb;5(2):159-61. (PMID: 18176568) Nat Phys. 2019 Jul 2;15(3):293-300. (PMID: 31327978) Science. 1993 Nov 26;262(5138):1401-7. (PMID: 8248779) J Cell Biol. 1990 Dec;111(6 Pt 1):2499-512. (PMID: 2277071) Methods. 2003 Aug;30(4):313-21. (PMID: 12828945) Exp Cell Res. 2014 Nov 1;328(2):258-66. (PMID: 25128809) Nat Methods. 2014 Jun;11(6):677-82. (PMID: 24727651) J Cell Biol. 2011 May 2;193(3):583-94. (PMID: 21518794) Cell. 1996 Nov 15;87(4):601-6. (PMID: 8929529) J Cell Sci. 2019 Jul 15;132(14):. (PMID: 31221727) Dev Cell. 2017 Aug 21;42(4):416-434.e11. (PMID: 28829947) Dev Cell. 2006 Feb;10(2):199-208. (PMID: 16459299) Mol Biol Cell. 2010 Jan 15;21(2):266-77. (PMID: 19923324) Nature. 1970 Feb 7;225(5232):553-4. (PMID: 4189355) WormBook. 2014 Dec 30;:1-43. (PMID: 25548889) J Biol Chem. 2005 May 20;280(20):20021-9. (PMID: 15781472) Nat Methods. 2015 Mar;12(3):244-50, 3 p following 250. (PMID: 25599551) Development. 2003 Apr;130(7):1255-65. (PMID: 12588843) Elife. 2022 Dec 19;11:. (PMID: 36533896) Curr Opin Cell Biol. 2020 Feb;62:123-134. (PMID: 31760155) Dev Cell. 2004 Sep;7(3):413-24. (PMID: 15363415) Curr Biol. 2010 Jul 27;20(14):1296-303. (PMID: 20579886) Cell. 2015 Apr 9;161(2):374-86. (PMID: 25799384) Science. 2011 Nov 25;334(6059):1137-41. (PMID: 22021673) J Biol Chem. 2003 Aug 15;278(33):31240-50. (PMID: 12756256) Science. 1990 Mar 9;247(4947):1229-33. (PMID: 2315695) Dev Cell. 2015 Oct 12;35(1):131-42. (PMID: 26460948) Biophys J. 2015 Feb 17;108(4):799-809. (PMID: 25692585) Development. 2006 Oct;133(19):3745-54. (PMID: 16943281) Nat Cell Biol. 2017 Aug;19(8):988-995. (PMID: 28737772) Biophys J. 2011 Sep 21;101(6):1412-22. (PMID: 21943422) Sci Rep. 2017 Oct 11;7(1):12970. (PMID: 29021607) Genetics. 1974 May;77(1):71-94. (PMID: 4366476) Development. 2019 Mar 25;146(6):. (PMID: 30814118) Curr Biol. 2003 Aug 5;13(15):1330-4. (PMID: 12906794) Science. 1988 Feb 19;239(4842):883-8. (PMID: 3277283) MicroPubl Biol. 2022 Aug 4;2022:. (PMID: 35996692) Cell Rep. 2022 Apr 12;39(2):110652. (PMID: 35417695) Dev Biol. 2010 Aug 15;344(2):745-57. (PMID: 20678977) Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12729-34. (PMID: 19622735) Cell Rep. 2016 Aug 23;16(8):2156-2168. (PMID: 27524610) Cell Rep. 2022 May 31;39(9):110868. (PMID: 35649363) Development. 2022 Jul 15;149(14):. (PMID: 35713287) Nature. 1989 Jul 27;340(6231):284-8. (PMID: 2747796) Biophys J. 2022 Dec 6;121(23):4543-4559. (PMID: 36815706) Proc Natl Acad Sci U S A. 2006 Jun 13;103(24):9045-50. (PMID: 16754851) EMBO J. 2007 Jun 6;26(11):2786-96. (PMID: 17476308) Development. 2017 Oct 1;144(19):3405-3416. (PMID: 28974638) Genetics. 2018 Nov;210(3):781-787. (PMID: 30213854) Curr Biol. 2017 Jan 9;27(1):103-112. (PMID: 27989674) |
| معلومات مُعتمدة: | P40 OD010440 United States OD NIH HHS; United Kingdom WT_ Wellcome Trust; BB/M011178/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; FC001086 United Kingdom ARC_ Arthritis Research UK; FC001086 United Kingdom WT_ Wellcome Trust; FC001086 United Kingdom MRC_ Medical Research Council; FC001086 United Kingdom CRUK_ Cancer Research UK |
| المشرفين على المادة: | 0 (Actins) 9013-26-7 (Actomyosin) 0 (Caenorhabditis elegans Proteins) EC 2.7.11.1 (PAR-3 protein, C elegans) EC 2.7.11.1 (Protein Serine-Threonine Kinases) |
| تواريخ الأحداث: | Date Created: 20230602 Date Completed: 20230605 Latest Revision: 20230619 |
| رمز التحديث: | 20230620 |
| مُعرف محوري في PubMed: | PMC10238861 |
| DOI: | 10.1083/jcb.202209111 |
| PMID: | 37265444 |
| قاعدة البيانات: | MEDLINE |
كن أول من يترك تعليقا!