دورية أكاديمية
Quaking regulates circular RNA production in cardiomyocytes.
| العنوان: | Quaking regulates circular RNA production in cardiomyocytes. |
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| المؤلفون: | Montañés-Agudo P; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands., van der Made I; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands., Aufiero S; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands., Tijsen AJ; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands., Pinto YM; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands., Creemers EE; Department of Experimental Cardiology, Amsterdam Cardiovascular Sciences, Amsterdam UMC location University of Amsterdam, 1105AZ, Amsterdam, The Netherlands. |
| المصدر: | Journal of cell science [J Cell Sci] 2023 Jul 01; Vol. 136 (13). Date of Electronic Publication: 2023 Jun 30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Company of Biologists Country of Publication: England NLM ID: 0052457 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1477-9137 (Electronic) Linking ISSN: 00219533 NLM ISO Abbreviation: J Cell Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Company of Biologists Original Publication: London. |
| مواضيع طبية MeSH: | RNA, Circular*/genetics , RNA, Circular*/metabolism , Myocytes, Cardiac*/metabolism, Mice ; Animals ; Alternative Splicing/genetics ; RNA Splicing ; Mice, Knockout ; RNA/genetics ; RNA/metabolism ; RNA-Binding Proteins/metabolism |
| مستخلص: | Circular RNAs (circRNAs) are a class of non-coding RNA molecules that are gaining increasing attention for their roles in various pathophysiological processes. The RNA-binding protein quaking (QKI) has been identified as a regulator of circRNA formation. In this study, we investigate the role of QKI in the formation of circRNAs in the heart by performing RNA-sequencing on Qki-knockout mice. Loss of QKI resulted in the differential expression of 17% of the circRNAs in adult mouse hearts. Interestingly, the majority of the QKI-regulated circRNAs (58%) were derived from genes undergoing QKI-dependent splicing, indicating a relationship between back-splicing and linear splicing. We compared these QKI-dependent circRNAs with those regulated by RBM20, another cardiac splicing factor essential for circRNA formation. We found that QKI and RBM20 regulate the formation of a distinct, but partially overlapping set of circRNAs in the heart. Strikingly, many shared circRNAs were derived from the Ttn gene, and they were regulated in an opposite manner. Our findings indicate that QKI not only regulates alternative splicing in the heart but also the formation of circRNAs. Competing Interests: Competing interests Y.M.P. is an inventor on patents, holds minor shares (<5%), and serves as a consultant for biotech and pharmaceutical companies that develop molecules or RNA therapies that target myocardial disease (Forbion Capital, ARMGO BV, Oxitope Pharmaceuticals and Phlox Therapeutics) and received support from Roche Diagnostics. The remaining authors have no conflicts of interest to declare. (© 2023. Published by The Company of Biologists Ltd.) |
| References: | Mol Cell. 2017 Apr 6;66(1):22-37.e9. (PMID: 28344082) RNA. 2018 Jun;24(6):815-827. (PMID: 29567830) Circ Res. 2001 Jul 6;89(1):20-5. (PMID: 11440973) Circulation. 2021 Apr 13;143(15):1502-1512. (PMID: 33583186) Cardiovasc Res. 2017 Mar 1;113(3):298-309. (PMID: 28082450) Mol Cell. 2017 Apr 6;66(1):9-21.e7. (PMID: 28344080) Eur Heart J. 2022 Nov 7;43(42):4496-4511. (PMID: 35758064) Stem Cell Res Ther. 2018 Mar 9;9(1):56. (PMID: 29523209) Sci Rep. 2016 Oct 11;6:34985. (PMID: 27725737) Nat Rev Cardiol. 2019 Aug;16(8):503-514. (PMID: 30952956) Cardiovasc Res. 2023 May 22;119(5):1161-1174. (PMID: 36627242) Bioinformatics. 2014 Aug 1;30(15):2114-20. (PMID: 24695404) Nat Commun. 2021 Jan 4;12(1):89. (PMID: 33397958) J Neurosci. 2016 Apr 06;36(14):4106-20. (PMID: 27053216) Genome Biol. 2014;15(12):550. (PMID: 25516281) Cardiovasc Res. 2022 Nov 10;118(14):2903-2918. (PMID: 34662387) Nature. 2012 Sep 6;489(7414):101-8. (PMID: 22955620) Nucleic Acids Res. 2013 Feb 1;41(4):2659-72. (PMID: 23307558) Cell. 2015 Mar 12;160(6):1125-34. (PMID: 25768908) J Mol Cell Cardiol. 2017 Aug;109:48-56. (PMID: 28676412) Cell Res. 2017 May;27(5):626-641. (PMID: 28281539) Circ Res. 2016 Oct 14;119(9):996-1003. (PMID: 27531932) J Clin Invest. 2014 Aug;124(8):3419-30. (PMID: 24960161) Genome Res. 2002 Jun;12(6):996-1006. (PMID: 12045153) Circ Res. 2018 Jan 19;122(2):246-254. (PMID: 29133306) Nucleic Acids Res. 2022 May 20;50(9):5313-5334. (PMID: 35544276) Cells. 2020 Jul 04;9(7):. (PMID: 32635460) BMC Bioinformatics. 2020 Apr 29;21(1):164. (PMID: 32349660) J Mol Cell Cardiol. 2016 Sep;98:103-7. (PMID: 27476877) Wiley Interdiscip Rev RNA. 2022 Nov;13(6):e1724. (PMID: 35298877) Genome Res. 2012 Oct;22(10):2008-17. (PMID: 22722343) Nucleic Acids Res. 2010 Oct;38(18):e178. (PMID: 20802226) J Mol Biol. 2012 Nov 9;423(5):766-81. (PMID: 22982292) Eur Heart J. 2017 May 07;38(18):1402-1412. (PMID: 26873092) J Mol Cell Cardiol. 2023 Jun;179:72-79. (PMID: 37059416) Nat Med. 2012 May;18(5):766-73. (PMID: 22466703) Cardiovasc Res. 2014 Oct 1;104(1):61-71. (PMID: 25103110) |
| معلومات مُعتمدة: | Rembrandt Institute of Cardiovascular Science; CVON ARENA-PRIME Netherlands Research Initiative; CVON-ARENA-PRIME Netherlands Cardiovascular Research Initiative CVON; University of Amsterdam |
| فهرسة مساهمة: | Keywords: Alternative splicing; Cardiomyocytes; Quaking; RNA-binding proteins; circRNAs |
| سلسلة جزيئية: | figshare 10.6084/m9.figshare.23056598.v1 |
| المشرفين على المادة: | 0 (RNA, Circular) 63231-63-0 (RNA) 0 (RBM20 protein, mouse) 0 (RNA-Binding Proteins) |
| تواريخ الأحداث: | Date Created: 20230605 Date Completed: 20230703 Latest Revision: 20240201 |
| رمز التحديث: | 20240201 |
| مُعرف محوري في PubMed: | PMC10323251 |
| DOI: | 10.1242/jcs.261120 |
| PMID: | 37272356 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1477-9137 |
|---|---|
| DOI: | 10.1242/jcs.261120 |