دورية أكاديمية
Direct Visualization of Structurally Similar Polysaccharides in Single Yeast Cells in Vivo by Multivariate Analysis Assisted Raman Microspectroscopy.
| العنوان: | Direct Visualization of Structurally Similar Polysaccharides in Single Yeast Cells in Vivo by Multivariate Analysis Assisted Raman Microspectroscopy. |
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| المؤلفون: | Hossain IM; The United Graduate School of Agricultural Sciences, Tottori University, Tottori 6808550, Japan., Mazumder N; Department of Biophysics, Manipal School of Life Sciences, Manipal Academy of Higher Education, Manipal, Karnataka 576104, India., Kaino T; Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-8504, Japan., Kawamukai M; Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-8504, Japan., Noothalapati H; Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-8504, Japan., Yamamoto T; Faculty of Life and Environmental Sciences, Shimane University, Matsue 690-8504, Japan. |
| المصدر: | The journal of physical chemistry. B [J Phys Chem B] 2023 Jun 15; Vol. 127 (23), pp. 5249-5256. Date of Electronic Publication: 2023 Jun 06. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Chemical Society Country of Publication: United States NLM ID: 101157530 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-5207 (Electronic) Linking ISSN: 15205207 NLM ISO Abbreviation: J Phys Chem B Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Washington, D.C. : American Chemical Society, c1997- |
| مواضيع طبية MeSH: | Saccharomyces cerevisiae* , beta-Glucans*/chemistry, Polysaccharides ; Glucans ; Multivariate Analysis |
| مستخلص: | The demand for functional food ingredients like β-glucan has risen enormously in recent times owing to its use in many fields including the food and beverage, cosmetics, pharmaceuticals, and biotechnology industries. Among the many natural sources of glucans such as oats, barley, mushrooms, and seaweeds, yeast has a special advantage in the industrial production of glucans. However, characterizing glucans is not straightforward as there are many different structural variations such as α- or β-glucans with various configurations which vary in their physical and chemical properties. Currently, microscopy, chemical or genetic approaches are followed to study glucan synthesis and accumulation in single yeast cells. However, they are time-consuming, lack molecular specificity, or are practically not feasible for real applications. Therefore, we developed a Raman microspectroscopy based method to identify, distinguish, and visualize structurally similar glucan polysaccharides. By employing multivariate curve resolution analysis, we successfully separated Raman spectra of α- and β-glucans from mixtures with high specificity and visualized heterogeneous molecular distributions during the sporulation of yeasts at the single-cell level in a label-free manner. We believe such an approach when combined with a flow cell can achieve the sorting of yeast cells based on the accumulation of glucans for various applications. Further, such an approach can also be extended to various other biological systems to investigate structurally similar carbohydrate polymers in a fast and reliable manner. |
| المشرفين على المادة: | 0 (Polysaccharides) 0 (Glucans) 0 (beta-Glucans) |
| تواريخ الأحداث: | Date Created: 20230606 Date Completed: 20230616 Latest Revision: 20230630 |
| رمز التحديث: | 20230630 |
| DOI: | 10.1021/acs.jpcb.3c00632 |
| PMID: | 37279109 |
| قاعدة البيانات: | MEDLINE |
Find this issue from the American Chemical Society | تدمد: | 1520-5207 |
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| DOI: | 10.1021/acs.jpcb.3c00632 |