دورية أكاديمية
أعرض في EDS

Development and nationwide validation of kidney graft injury markers using urinary exosomes and microvesicles (complete English translation of the Japanese version).

التفاصيل البيبلوغرافية
العنوان: Development and nationwide validation of kidney graft injury markers using urinary exosomes and microvesicles (complete English translation of the Japanese version).
المؤلفون: Harada H; Department of Kidney Transplant Surgery, Sapporo City General Hospital, 1-1 Kita 11-jo Nishi 13-chome, Chuou- ku, Sapporo, Hokkaido, 060-8604, Japan. hirohara80@mac.com.; Harada Urological Clinic, 4F Hokuyaku Bldg., 1-1 Kita 11-jo Nishi 14-chome, Chuou-ku, Sapporo, Hokkaido, 060-0011, Japan. hirohara80@mac.com., Fukuzawa N; Department of Kidney Transplant Surgery, Sapporo City General Hospital, 1-1 Kita 11-jo Nishi 13-chome, Chuou- ku, Sapporo, Hokkaido, 060-8604, Japan., Abe T; Department of Urology, Graduate School of Medicine, Faculty of Medicine, Osaka University, 1 Machikaneyama- cho, Toyonaka, Osaka, 560-0043, Japan., Imamura R; Department of Urology, Graduate School of Medicine, Faculty of Medicine, Osaka University, 1 Machikaneyama- cho, Toyonaka, Osaka, 560-0043, Japan., Masaki N; Department of Kidney Surgery, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162- 8666, Japan., Fujiyama N; Department of Center for Kidney Disease and Transplantation, Akita University Hospital, 44-2 Hiroomote Azahasunuma, Akita, Akita, 010-8543, Japan., Sato S; Department of Center for Kidney Disease and Transplantation, Akita University Hospital, 44-2 Hiroomote Azahasunuma, Akita, Akita, 010-8543, Japan., Hatakeyama S; Department of Urology, Hirosaki University Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, Aomori, 036-8562, Japan., Nishimura K; Department of Urology, Hyogo Prefectural Nishinomiya Hospital, 13-9 Rokutanji-cho, Nishinomiya, Hyogo, Japan., Kishikawa H; Department of Urology, Hyogo Prefectural Nishinomiya Hospital, 13-9 Rokutanji-cho, Nishinomiya, Hyogo, Japan., Iwami D; Division of Renal Surgery and Transplantation, Department of Urology, Jichi Medical University, 3311-1, Yakushiji, Shimotsuke, Tochigi, 329-0498, Japan., Hotta K; Department of Renal and Genitourinary Surgery, Graduate School of Medicine, Hokkaido University, Kita 15-jo Nishi 7-chome, Kita-ku, Sapporo, Hokkaido, 060-8638, Japan., Miura M; Department of Kidney Transplant Surgery, Sapporo Hokuyu Hospital, 5-1 Higashi-sapporo 6-jo 6-chome, Shiroishi- ku, Sapporo, Hokkaido, 003-0006, Japan., Ide K; Department of Gastroenterological and Transplant Surgery, Graduate School of Biochemical and Health Sciences, Hiroshima University, 1-2-3 Kasumi, Minami-ku, Hiroshima, 734-8553, Japan., Nakamura M; Department of Transplant Surgery, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa, 259-1193, Japan., Kosoku A; Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka, Osaka, 545- 8585, Japan., Uchida J; Department of Urology, Osaka Metropolitan University Graduate School of Medicine, 1-4-3, Asahi-Machi, Abeno-ku, Osaka, Osaka, 545- 8585, Japan., Murakami T; R&D Center, Hitachi Chemical Co. America, Ltd. 1003 Health Sciences Road, Irvine, CA, 92617, USA., Tsuji T; Department of Pathology, Sapporo City General Hospital, 1-1 Kita 11-jo Nishi 13-chome, Chuou-ku, Sapporo, Hokkaido, 060-8604, Japan.
المصدر: BMC nephrology [BMC Nephrol] 2023 Jun 06; Vol. 24 (1), pp. 158. Date of Electronic Publication: 2023 Jun 06.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967793 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2369 (Electronic) Linking ISSN: 14712369 NLM ISO Abbreviation: BMC Nephrol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2000-
مواضيع طبية MeSH: Exosomes* , Kidney Diseases*/pathology , Kidney Transplantation*/adverse effects, Humans ; Antibodies ; Biomarkers/urine ; Graft Rejection/genetics ; Kidney/pathology ; RNA ; Japan
مستخلص: Background: Non-invasive, prompt, and proper detection tools for kidney graft injuries (KGIs) are awaited to ensure graft longevity. We screened diagnostic biomarkers for KGIs following kidney transplantation using extracellular vesicles (EVs; exosomes and microvesicles) from the urine samples of patients.
Methods: One hundred and twenty-seven kidney recipients at 11 Japanese institutions were enrolled in this study; urine samples were obtained prior to protocol/episode biopsies. EVs were isolated from urine samples, and EV RNA markers were assayed using quantitative reverse transcription polymerase chain reaction. Diagnostic performance of EV RNA markers and diagnostic formulas comprising these markers were evaluated by comparison with the corresponding pathological diagnoses.
Results: EV CXCL9, CXCL10, and UMOD were elevated in T-cell-mediated rejection samples compared with other KGI samples, while SPNS2 was elevated in chronic antibody-mediated rejection (cABMR) samples. A diagnostic formula developed through Sparse Logistic Regression analysis using EV RNA markers allowed us to accurately (with an area under the receiver operator characteristic curve [AUC] of 0.875) distinguish cABMR from other KGI samples. EV B4GALT1 and SPNS2 were also elevated in cABMR, and a diagnostic formula using these markers was able to distinguish between cABMR and chronic calcineurin toxicity accurately (AUC 0.886). In interstitial fibrosis and tubular atrophy (IFTA) urine samples and those with high Banff chronicity score sums (BChS), POTEM levels may reflect disease severity, and diagnostic formulas using POTEM detected IFTA (AUC 0.830) and high BChS (AUC 0.850).
Conclusions: KGIs could be diagnosed with urinary EV mRNA analysis with relatively high accuracy.
(© 2023. The Author(s).)
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فهرسة مساهمة: Keywords: Biomarker; Extracellular vesicle; Kidney transplant; Urine; mRNA
المشرفين على المادة: 0 (Antibodies)
0 (Biomarkers)
17911-61-4 (bis(cyclohexylammonium)sulfate)
63231-63-0 (RNA)
تواريخ الأحداث: Date Created: 20230606 Date Completed: 20230608 Latest Revision: 20230614
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10242781
DOI: 10.1186/s12882-023-03189-z
PMID: 37280521
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2369
DOI:10.1186/s12882-023-03189-z