دورية أكاديمية
أعرض في EDS

A high-throughput cytotoxicity screening platform reveals agr -independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence.

التفاصيل البيبلوغرافية
العنوان: A high-throughput cytotoxicity screening platform reveals agr -independent mutations in bacteraemia-associated Staphylococcus aureus that promote intracellular persistence.
المؤلفون: Hachani A; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Giulieri SG; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Guérillot R; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Walsh CJ; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Herisse M; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Soe YM; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Baines SL; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Thomas DR; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia.; Infection and Immunity Program, Department of Microbiology and Biomedicine Discovery Institute, Monash University, Clayton, Australia., Cheung SD; Biological Optical Microscopy Platform, University of Melbourne, Melbourne, Australia., Hayes AS; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Cho E; Biological Optical Microscopy Platform, University of Melbourne, Melbourne, Australia., Newton HJ; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia.; Infection and Immunity Program, Department of Microbiology and Biomedicine Discovery Institute, Monash University, Clayton, Australia., Pidot S; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Massey RC; School of Microbiology, University College Cork, Cork, Ireland.; School of Medicine, University College Cork, Cork, Ireland.; APC Microbiome Ireland, University College Cork, Cork, Ireland.; School of Cellular and Molecular Medicine, University of Bristol, Bristol, United Kingdom., Howden BP; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia.; Microbiological Diagnostic Unit Public Health Laboratory, Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia., Stinear TP; Department of Microbiology and Immunology, Doherty Institute, University of Melbourne, Melbourne, Australia.
المصدر: ELife [Elife] 2023 Jun 08; Vol. 12. Date of Electronic Publication: 2023 Jun 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: eLife Sciences Publications, Ltd Country of Publication: England NLM ID: 101579614 Publication Model: Electronic Cited Medium: Internet ISSN: 2050-084X (Electronic) Linking ISSN: 2050084X NLM ISO Abbreviation: Elife Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Cambridge, UK : eLife Sciences Publications, Ltd., 2012-
مواضيع طبية MeSH: Staphylococcal Infections*/microbiology , Bacteremia*/microbiology, Humans ; Staphylococcus aureus/metabolism ; Mutation ; Cell Line ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism
مستخلص: Staphylococcus aureus infections are associated with high mortality rates. Often considered an extracellular pathogen, S. aureus can persist and replicate within host cells, evading immune responses, and causing host cell death. Classical methods for assessing S. aureus cytotoxicity are limited by testing culture supernatants and endpoint measurements that do not capture the phenotypic diversity of intracellular bacteria. Using a well-established epithelial cell line model , we have developed a platform called InToxSa ( in tracellular tox icity of S. a ureus ) to quantify intracellular cytotoxic S. aureus phenotypes. Studying a panel of 387 S . aureus bacteraemia isolates, and combined with comparative, statistical, and functional genomics, our platform identified mutations in S. aureus clinical isolates that reduced bacterial cytotoxicity and promoted intracellular persistence. In addition to numerous convergent mutations in the Agr quorum sensing system, our approach detected mutations in other loci that also impacted cytotoxicity and intracellular persistence. We discovered that clinical mutations in ausA , encoding the aureusimine non-ribosomal peptide synthetase, reduced S. aureus cytotoxicity, and increased intracellular persistence. InToxSa is a versatile, high-throughput cell-based phenomics platform and we showcase its utility by identifying clinically relevant S. aureus pathoadaptive mutations that promote intracellular residency.
Competing Interests: AH, SG, RG, CW, MH, YS, SB, DT, SC, AH, EC, HN, SP, RM, BH, TS No competing interests declared
(© 2023, Hachani, Giulieri, Guérillot et al.)
References: Infect Immun. 2005 Apr;73(4):2273-80. (PMID: 15784571)
J Immunol. 2014 May 15;192(10):4709-17. (PMID: 24729616)
mBio. 2019 Oct 15;10(5):. (PMID: 31615966)
J Exp Med. 2016 Jun 27;213(7):1141-51. (PMID: 27325887)
Antimicrob Agents Chemother. 2022 Jan 18;66(1):e0155921. (PMID: 34748383)
J Clin Invest. 2019 Nov 1;129(11):4643-4656. (PMID: 31545300)
Nat Rev Microbiol. 2015 Sep;13(9):529-43. (PMID: 26272408)
Microbiologyopen. 2021 Mar;10(2):e1178. (PMID: 33970534)
Infect Immun. 2022 May 19;90(5):e0061421. (PMID: 35467397)
Proc Natl Acad Sci U S A. 2016 May 31;113(22):E3101-10. (PMID: 27185949)
Biochemistry. 2013 Feb 5;52(5):926-37. (PMID: 23302043)
Genome Med. 2018 Aug 23;10(1):65. (PMID: 30103826)
Elife. 2022 Jun 14;11:. (PMID: 35699423)
J Infect Dis. 2015 Jun 15;211(12):1862-74. (PMID: 25298028)
Nat Rev Microbiol. 2011 Mar;9(3):215-22. (PMID: 21297670)
Microb Genom. 2020 Mar;6(3):. (PMID: 32100713)
Microb Genom. 2019 Jan;5(1):. (PMID: 30663960)
Cell Microbiol. 2019 Sep;21(9):e13044. (PMID: 31099148)
Microbiology (Reading). 2021 Dec;167(12):. (PMID: 34928202)
Lancet. 2022 Feb 12;399(10325):629-655. (PMID: 35065702)
Bioinformatics. 2018 Dec 15;34(24):4310-4312. (PMID: 30535304)
Nat Microbiol. 2016 Apr 04;1:16041. (PMID: 27572646)
Antimicrob Agents Chemother. 2011 Mar;55(3):1082-7. (PMID: 21173172)
mBio. 2015 Mar 03;6(2):e00080. (PMID: 25736880)
Chem Biol. 2010 Sep 24;17(9):925-30. (PMID: 20851341)
J Infect Dis. 2011 Aug 1;204(3):340-7. (PMID: 21742831)
Nat Microbiol. 2022 Nov;7(11):1791-1804. (PMID: 36216891)
PLoS Pathog. 2014 Jun 05;10(6):e1004169. (PMID: 24901708)
Clin Microbiol Rev. 2015 Jul;28(3):603-61. (PMID: 26016486)
Genome Biol. 2018 Jul 18;19(1):94. (PMID: 30021593)
Nature. 2022 Apr;604(7906):502-508. (PMID: 35396580)
mBio. 2020 Dec 15;11(6):. (PMID: 33323513)
Infect Immun. 2019 Apr 23;87(5):. (PMID: 30782857)
Nat Rev Microbiol. 2014 Jan;12(1):49-62. (PMID: 24336184)
mBio. 2018 Jul 17;9(4):. (PMID: 30018109)
Elife. 2017 Dec 19;6:. (PMID: 29256859)
Microb Genom. 2020 Nov;6(11):. (PMID: 33206035)
mBio. 2013 Feb 12;4(1):e00537-12. (PMID: 23404398)
Nat Genet. 2012 Jun 17;44(7):821-4. (PMID: 22706312)
Int J Med Microbiol. 2010 Feb;300(2-3):170-5. (PMID: 19781990)
Infect Immun. 2018 Sep 21;86(10):. (PMID: 30061376)
J Biol Chem. 2019 Nov 8;294(45):17031-17042. (PMID: 31558608)
J Leukoc Biol. 2013 Oct;94(4):595-601. (PMID: 23625199)
PLoS Biol. 2015 Sep 02;13(9):e1002229. (PMID: 26331877)
Annu Rev Microbiol. 2013;67:629-50. (PMID: 23834243)
PLoS Pathog. 2021 Sep 2;17(9):e1009874. (PMID: 34473800)
BMC Infect Dis. 2018 Mar 5;18(1):107. (PMID: 29506483)
Genome Res. 2014 May;24(5):839-49. (PMID: 24717264)
J Clin Microbiol. 2014 Sep;52(9):3384-93. (PMID: 25031442)
Science. 2004 Mar 5;303(5663):1532-5. (PMID: 15001782)
PLoS Pathog. 2018 Jun 14;14(6):e1007112. (PMID: 29902272)
Cell Microbiol. 2014 Apr;16(4):451-65. (PMID: 24164701)
J Negat Results Biomed. 2017 Feb 19;16(1):2. (PMID: 28214466)
J Leukoc Biol. 2020 Sep;108(3):967-981. (PMID: 32531864)
Cell Microbiol. 2021 May;23(5):e13317. (PMID: 33550697)
Genome Res. 2005 Jul;15(7):1015-22. (PMID: 15998914)
PeerJ. 2021 Jul 13;9:e11764. (PMID: 34306830)
PLoS One. 2009 Oct 06;4(10):e7363. (PMID: 19806211)
J Leukoc Biol. 2020 Jul;108(1):377-396. (PMID: 32202340)
PLoS Pathog. 2016 Sep 15;12(9):e1005857. (PMID: 27632173)
J Comput Biol. 2012 May;19(5):455-77. (PMID: 22506599)
Microb Genom. 2015 Aug 3;1(2):e000026. (PMID: 28348811)
PLoS Genet. 2022 Mar 24;18(3):e1010112. (PMID: 35324915)
Microbiol Spectr. 2019 Mar;7(2):. (PMID: 30873936)
Infect Immun. 2010 Aug;78(8):3392-403. (PMID: 20530231)
Microbiol Spectr. 2022 Aug 31;10(4):e0101122. (PMID: 35863033)
Proc Natl Acad Sci U S A. 2022 Aug 2;119(31):e2123017119. (PMID: 35881802)
Sci Rep. 2016 Jun 14;6:27899. (PMID: 27296890)
Virulence. 2021 Dec;12(1):547-569. (PMID: 33522395)
Nucleic Acids Res. 2012 Apr;40(8):e55. (PMID: 22241772)
Cell Microbiol. 2016 Apr;18(4):514-35. (PMID: 26408990)
Clin Microbiol Infect. 2016 Sep;22(9):799-809. (PMID: 27393124)
Front Microbiol. 2019 Sep 24;10:2242. (PMID: 31608046)
Immunol Cell Biol. 2020 Aug;98(7):577-594. (PMID: 32333812)
Mol Biol Evol. 2021 Mar 9;38(3):1101-1121. (PMID: 33118035)
mSphere. 2018 Jan 24;3(1):. (PMID: 29404415)
J Vis Exp. 2020 Jan 3;(155):. (PMID: 31957747)
Int J Med Microbiol. 2018 Aug;308(6):558-568. (PMID: 29198880)
Genome Biol. 2019 Nov 28;20(1):257. (PMID: 31779668)
Genome Biol. 2016 Jun 20;17(1):132. (PMID: 27323842)
J Infect Dis. 2004 Sep 15;190(6):1140-9. (PMID: 15319865)
Nat Microbiol. 2017 Oct;2(10):1381-1388. (PMID: 28785103)
Cell Microbiol. 2019 Mar;21(3):e12997. (PMID: 30576050)
Nat Rev Microbiol. 2016 Mar;14(3):150-62. (PMID: 26806595)
Bioinformatics. 2014 Jul 15;30(14):2068-9. (PMID: 24642063)
Access Microbiol. 2021 Jan 7;3(2):000193. (PMID: 34151146)
EMBO Rep. 2019 Dec 5;20(12):e48109. (PMID: 31637841)
Nat Commun. 2022 Jan 26;13(1):509. (PMID: 35082278)
J Clin Microbiol. 2008 Jun;46(6):2112-4. (PMID: 18417658)
Virulence. 2021 Dec;12(1):1186-1198. (PMID: 33843450)
Nature. 2022 Apr;604(7906):509-516. (PMID: 35396579)
Proc Natl Acad Sci U S A. 2012 Mar 20;109(12):4550-5. (PMID: 22393007)
Nat Commun. 2020 May 4;11(1):2200. (PMID: 32366839)
Sci Rep. 2016 Aug 11;6:31360. (PMID: 27511873)
mBio. 2015 May 26;6(3):e00308-15. (PMID: 26015493)
Stat Anal Data Min. 2021 Apr;14(2):144-167. (PMID: 33833846)
معلومات مُعتمدة: GNT2018880 National Health and Medical Research Council; GNT1105525 National Health and Medical Research Council; GNT1145631 National Health and Medical Research Council; GNT1196103 National Health and Medical Research Council
فهرسة مساهمة: Keywords: GWAS; Staphylococcus aureus; bacterial population genomics; cytotoxicity; evolutionary convergence analysis; genetics; genomics; infectious disease; intracellular; microbiology
المشرفين على المادة: 0 (Bacterial Proteins)
تواريخ الأحداث: Date Created: 20230608 Date Completed: 20230612 Latest Revision: 20231116
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10259494
DOI: 10.7554/eLife.84778
PMID: 37289634
قاعدة البيانات: MEDLINE
الوصف
تدمد:2050-084X
DOI:10.7554/eLife.84778