دورية أكاديمية
The Western Lake Erie culture collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome.
| العنوان: | The Western Lake Erie culture collection: A promising resource for evaluating the physiological and genetic diversity of Microcystis and its associated microbiome. |
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| المؤلفون: | Yancey CE; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Kiledal EA; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Chaganti SR; Cooperative Institute for Great Lakes Research (CIGLR), University of Michigan, 4840 S State Road, Ann Arbor, MI 48108, United States of America., Denef VJ; Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, United States of America., Errera RM; National Oceanic and Atmospheric Administration (NOAA), Great Lakes Environmental Research Laboratory (GLERL), 4840 S State Road, Ann Arbor, MI 48108, United States of America., Evans JT; Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, United States of America., Hart LN; Program in Chemical Biology, University of Michigan, Ann Arbor, MI 48109, United States of America; Life Sciences Institute, University of Michigan, Ann Arbor, MI 48109, United States of America., Isailovic D; Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH 43606, United States of America., James WS; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Kharbush JJ; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Kimbrel JA; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, United States of America., Li W; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, United States of America., Mayali X; Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA 94550, United States of America., Nitschky H; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Polik CA; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Powers MA; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Premathilaka SH; Department of Chemistry and Biochemistry, University of Toledo, Toledo, OH 43606, United States of America., Rappuhn NA; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Reitz LA; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Rivera SR; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Zwiers CC; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America., Dick GJ; Earth and Environmental Sciences, University of Michigan, Ann Arbor, MI 48109, United States of America; Cooperative Institute for Great Lakes Research (CIGLR), University of Michigan, 4840 S State Road, Ann Arbor, MI 48108, United States of America. Electronic address: gdick@umich.edu. |
| المصدر: | Harmful algae [Harmful Algae] 2023 Jul; Vol. 126, pp. 102440. Date of Electronic Publication: 2023 Apr 30. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 101128968 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-1470 (Electronic) Linking ISSN: 15689883 NLM ISO Abbreviation: Harmful Algae Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c2002- |
| مواضيع طبية MeSH: | Microcystis*/genetics , Cyanobacteria*/genetics , Microbiota*, Humans ; Lakes/microbiology ; Genetic Variation |
| مستخلص: | Cyanobacterial harmful algal blooms (cyanoHABs) dominated by Microcystis spp. have significant public health and economic implications in freshwater bodies around the world. These blooms are capable of producing a variety of cyanotoxins, including microcystins, that affect fishing and tourism industries, human and environmental health, and access to drinking water. In this study, we isolated and sequenced the genomes of 21 primarily unialgal Microcystis cultures collected from western Lake Erie between 2017 and 2019. While some cultures isolated in different years have a high degree of genetic similarity (genomic Average Nucleotide Identity >99%), genomic data show that these cultures also represent much of the breadth of known Microcystis diversity in natural populations. Only five isolates contained all the genes required for microcystin biosynthesis while two isolates contained a previously described partial mcy operon. Microcystin production within cultures was also assessed using Enzyme-Linked Immunosorbent Assay (ELISA) and supported genomic results with high concentrations (up to 900 μg L⁻¹) in cultures with complete mcy operons and no or low toxin detected otherwise. These xenic cultures also contained a substantial diversity of bacteria associated with Microcystis, which has become increasingly recognized as an essential component of cyanoHAB community dynamics. These results highlight the genomic diversity among Microcystis strains and associated bacteria in Lake Erie, and their potential impacts on bloom development, toxin production, and toxin degradation. This culture collection significantly increases the availability of environmentally relevant Microcystis strains from temperate North America. Competing Interests: Declaration of Competing Interest The authors declare no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023. Published by Elsevier B.V.) |
| فهرسة مساهمة: | Keywords: CyanoHABs; Metagenomics; Microcystin; Microcystis; Phycosphere; Western Lake Erie |
| تواريخ الأحداث: | Date Created: 20230608 Date Completed: 20230612 Latest Revision: 20231116 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/j.hal.2023.102440 |
| PMID: | 37290887 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-1470 |
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| DOI: | 10.1016/j.hal.2023.102440 |