دورية أكاديمية
Super-resolved trajectory-derived nanoclustering analysis using spatiotemporal indexing.
| العنوان: | Super-resolved trajectory-derived nanoclustering analysis using spatiotemporal indexing. |
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| المؤلفون: | Wallis TP; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia. t.wallis@uq.edu.au., Jiang A; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia., Young K; School of Mathematics and Physics, The University of Queensland, Brisbane, QLD, 4072, Australia., Hou H; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia., Kudo K; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia., McCann AJ; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia., Durisic N; Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia., Joensuu M; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia.; Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane, QLD, 4072, Australia., Oelz D; School of Mathematics and Physics, The University of Queensland, Brisbane, QLD, 4072, Australia., Nguyen H; School of Mathematics and Physics, The University of Queensland, Brisbane, QLD, 4072, Australia., Gormal RS; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia. r.gormal@uq.edu.au., Meunier FA; Clem Jones Centre for Ageing Dementia Research, Queensland Brain Institute, The University of Queensland, Brisbane, QLD, 4072, Australia. f.meunier@uq.edu.au.; School of Biomedical Sciences, The University of Queensland, Brisbane, QLD, 4072, Australia. f.meunier@uq.edu.au. |
| المصدر: | Nature communications [Nat Commun] 2023 Jun 08; Vol. 14 (1), pp. 3353. Date of Electronic Publication: 2023 Jun 08. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Nature Pub. Group Country of Publication: England NLM ID: 101528555 Publication Model: Electronic Cited Medium: Internet ISSN: 2041-1723 (Electronic) Linking ISSN: 20411723 NLM ISO Abbreviation: Nat Commun Subsets: PubMed not MEDLINE; MEDLINE |
| أسماء مطبوعة: | Original Publication: [London] : Nature Pub. Group |
| مواضيع طبية MeSH: | Proteins*/metabolism , Algorithms*, Cell Membrane/metabolism ; Spatio-Temporal Analysis |
| مستخلص: | Single-molecule localization microscopy techniques are emerging as vital tools to unravel the nanoscale world of living cells by understanding the spatiotemporal organization of protein clusters at the nanometer scale. Current analyses define spatial nanoclusters based on detections but neglect important temporal information such as cluster lifetime and recurrence in "hotspots" on the plasma membrane. Spatial indexing is widely used in video games to detect interactions between moving geometric objects. Here, we use the R-tree spatial indexing algorithm to determine the overlap of the bounding boxes of individual molecular trajectories to establish membership in nanoclusters. Extending the spatial indexing into the time dimension allows the resolution of spatial nanoclusters into multiple spatiotemporal clusters. Using spatiotemporal indexing, we found that syntaxin1a and Munc18-1 molecules transiently cluster in hotspots, offering insights into the dynamics of neuroexocytosis. Nanoscale spatiotemporal indexing clustering (NASTIC) has been implemented as a free and open-source Python graphic user interface. (© 2023. The Author(s).) |
| التعليقات: | Erratum in: Nat Commun. 2023 Jul 25;14(1):4468. (PMID: 37491417) |
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(PMID: 23646160363990110.1371/journal.pone.0062918) |
| معلومات مُعتمدة: | R21 AG080435 United States AG NIA NIH HHS |
| المشرفين على المادة: | 0 (Proteins) |
| تواريخ الأحداث: | Date Created: 20230608 Date Completed: 20230612 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC10250379 |
| DOI: | 10.1038/s41467-023-38866-y |
| PMID: | 37291117 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 2041-1723 |
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| DOI: | 10.1038/s41467-023-38866-y |