دورية أكاديمية
Analysis of nuclear maturation, DNA damage and repair gene expression of bovine oocyte and cumulus cells submitted to ionizing radiation.
العنوان: | Analysis of nuclear maturation, DNA damage and repair gene expression of bovine oocyte and cumulus cells submitted to ionizing radiation. |
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المؤلفون: | Rovani BT; Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Rissi VB; Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Rovani MT; Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Gasperin BG; Laboratório de Reprodução Animal, Grupo FiBRA, Universidade Federal de Pelotas, Pelotas, RS, Brasil., Baumhardt T; Serviço de Radioterapia, Hospital Universitário de Santa Maria, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Bordignon V; Department of Animal Science, McGill University, Sainte Anne de Bellevue, QC, Canada., Bauermann LF; Departamento de Fisiologia e Farmacologia, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Missio D; Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil., Gonçalves PBD; Laboratório de Biotecnologia e Reprodução Animal, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil. |
المصدر: | Animal reproduction [Anim Reprod] 2023 May 29; Vol. 20 (2), pp. e20230021. Date of Electronic Publication: 2023 May 29 (Print Publication: 2023). |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Colégio Brasileiro de Reprodução Animal Country of Publication: Brazil NLM ID: 101272804 Publication Model: eCollection Cited Medium: Internet ISSN: 1984-3143 (Electronic) Linking ISSN: 18069614 NLM ISO Abbreviation: Anim Reprod Subsets: PubMed not MEDLINE |
أسماء مطبوعة: | Original Publication: Belo Horizonte, MG, Brazil : Colégio Brasileiro de Reprodução Animal, [2004]- |
مستخلص: | Radiotherapy causes destruction of tumor cells, but also threatens the integrity and survival of surrounding normal cells. Then, woman submitted to irradiation for cancer treatment may present permanent ovary damage, resulting in impaired fertility. The objective of this study was to investigate the effects of therapeutic doses of ionizing radiation (IR), used for ovarian cancer treatment in humans, on bovine cumulus-oocyte complexes (COCs) as experimental model. Bovine ovaries were exposed to 0.9 Gy, 1.8 Gy, 3.6 Gy or 18.6 Gy IR, and then COCs were collected and used to evaluate: (a) oocyte nuclear maturation; (b) presence of phosphorylated H2A.X (γH2AX), as an indicator of DNA double-strand breaks (DSBs); and (c) expression of genes involved in DNA repair ( TP53BP1, RAD52, ATM, XRCC6 and XRCC5 ) and apoptosis ( BAX ). The radiation doses tested in this study had no detrimental effects on nuclear maturation and did not increase γH2AX in the oocytes. However, IR treatment altered the mRNA abundance of RAD52 (RAD52 homolog, DNA repair protein) and BAX (BCL2-associated X protein). We conclude that although IR doses had no apparent effect on oocyte nuclear maturation and DNA damage, molecular pathways involved in DNA repair and apoptosis were affected by IR exposure in cumulus cells. Competing Interests: Conflicts of interest: The authors have no conflict of interest to declare. |
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فهرسة مساهمة: | Keywords: DNA damage; infertility; ionizing radiation; oocyte |
تواريخ الأحداث: | Date Created: 20230609 Latest Revision: 20230612 |
رمز التحديث: | 20240628 |
مُعرف محوري في PubMed: | PMC10247184 |
DOI: | 10.1590/1984-3143-AR2023-0021 |
PMID: | 37293252 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1984-3143 |
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DOI: | 10.1590/1984-3143-AR2023-0021 |