دورية أكاديمية
أعرض في EDS

Viperin from the dromedary camel: First report of an antiviral interferon-responsive gene from camelids.

التفاصيل البيبلوغرافية
العنوان: Viperin from the dromedary camel: First report of an antiviral interferon-responsive gene from camelids.
المؤلفون: Premraj A; Camel Biotechnology Center, Presidential Camels & Camel Racing Affairs Centre, Department of the President's Affairs, PO Box 17292, Al Ain, United Arab Emirates., Aleyas AG; Camel Biotechnology Center, Presidential Camels & Camel Racing Affairs Centre, Department of the President's Affairs, PO Box 17292, Al Ain, United Arab Emirates., Nautiyal B; Camel Biotechnology Center, Presidential Camels & Camel Racing Affairs Centre, Department of the President's Affairs, PO Box 17292, Al Ain, United Arab Emirates., Rasool TJ; Camel Biotechnology Center, Presidential Camels & Camel Racing Affairs Centre, Department of the President's Affairs, PO Box 17292, Al Ain, United Arab Emirates. Electronic address: tjrasool@gmail.com.
المصدر: Developmental and comparative immunology [Dev Comp Immunol] 2023 Oct; Vol. 147, pp. 104754. Date of Electronic Publication: 2023 Jun 07.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: United States NLM ID: 7708205 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0089 (Electronic) Linking ISSN: 0145305X NLM ISO Abbreviation: Dev Comp Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: Tarrytown Ny : Elsevier Science
Original Publication: Elmsford, N. Y., Pergamon Press., 1977-2004.
مواضيع طبية MeSH: Interferons*/genetics , Antiviral Agents*/metabolism, Animals ; Camelus ; Amino Acids ; Antiviral Restriction Factors
مستخلص: Viral infections activate pattern recognition receptors in the host, triggering an innate immune response that involves the production of interferons, which, in turn, stimulates the expression of antiviral effector genes. Viperin is one of the most highly induced interferon-stimulated genes and displays broad antiviral activity, especially against tick-borne viruses. Of late, camelid-borne zoonotic viruses have been on the rise in the Arabian Peninsula, but research into camelid antiviral effector genes has been limited. This is the first report of an interferon-responsive gene from the mammalian suborder Tylopoda to which modern camels belong. From camel kidney cells treated with dsRNA mimetic, we cloned viperin cDNA encoding 361 amino acid protein. Sequence analysis of camel viperin reveals high levels of amino acid conservation, particularly within the RSAD domain. Compared to kidney, the relative mRNA expression of viperin was higher in blood, lung, spleen, lymph nodes, and intestines. The in-vitro expression of viperin was induced by poly(I:C) and interferon treatment in camel kidney cell lines. Viperin expression was subdued in camel kidney cells infected with the camelpox virus during the early stages of infection, suggesting possible suppression by the virus. Overexpression of camel viperin through transient transfection significantly enhanced the resistance of cultured camel kidney cell lines to infection with camelpox virus. Research into the role of viperin in host immunity against emerging viral pathogens of camels will provide insight into novel mechanisms of antiviral activity of the protein, viral immune evasion strategies, and enable the development of better antivirals.
Competing Interests: Conflict of interest The authors declare that they have no conflicts of interest.
(Copyright © 2023 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Antiviral; Camel; Emerging viral pathogen; Pox virus; RSAD2; Zoonotic
المشرفين على المادة: 9008-11-1 (Interferons)
0 (Antiviral Agents)
0 (Amino Acids)
0 (Antiviral Restriction Factors)
تواريخ الأحداث: Date Created: 20230609 Date Completed: 20230809 Latest Revision: 20230811
رمز التحديث: 20231215
DOI: 10.1016/j.dci.2023.104754
PMID: 37295628
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0089
DOI:10.1016/j.dci.2023.104754