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Biotech Application of Exopolysaccharides from Curvularia brachyspora : Optimization of Production, Structural Characterization, and Biological Activity.

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العنوان: Biotech Application of Exopolysaccharides from Curvularia brachyspora : Optimization of Production, Structural Characterization, and Biological Activity.
المؤلفون: Menolli RA; Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel 85819-110, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Tessaro FHG; Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, São Paulo 05508-000, SP, Brazil., do Amaral AE; Unidade de Laboratório de Análises Clínicas, Universidade Federal de Santa Catarina, Florianópolis 88036-800, SC, Brazil., de Melo RH; Center of Medical and Pharmaceutical Sciences, Western Parana State University, Cascavel 85819-110, PR, Brazil., Dos Santos JF; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Iacomini M; Department of Biochemistry and Molecular Biology, Federal University of Paraná, Curitiba 81531-980, PR, Brazil., Smiderle FR; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil., Mello RG; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil.; Instituto de Pesquisa Pelé Pequeno Príncipe, Curitiba 80240-020, PR, Brazil.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2023 May 26; Vol. 28 (11). Date of Electronic Publication: 2023 May 26.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: Polysaccharides*/pharmacology , Macrophages*, Animals ; Mice ; Spectroscopy, Fourier Transform Infrared ; Biotechnology
مستخلص: C. brachyspora , a widespread dematiaceous fungus, was evaluated in this study to optimize the production of exopolysaccharides (CB-EPS). Optimization was performed using response surface methodology, and the best production yielded 75.05% of total sugar at pH 7.4, with 0.1% urea, after 197 h. The obtained CB-EPS showed typical signals of polysaccharides, which was confirmed by FT-IR and NMR. The HPSEC analysis indicated a polydisperse polymer, showing a non-uniform peak, with an average molar mass (M w ) of 24,470 g/mol. The major monosaccharide was glucose (63.9 Mol%), followed by mannose (19.7 Mol%), and galactose (16.4 Mol%). Methylation analysis encountered derivatives that indicated the presence of a β-d-glucan and a highly branched glucogalactomannan. CB-EPS was tested on murine macrophages to verify its immunoactivity, and the treated cells were able to produce TNF-α, IL-6, and IL-10. However, the cells did not produce superoxide anions or nitric oxide nor stimulated phagocytosis. The results demonstrated an indirect antimicrobial activity of macrophages by stimulating cytokines, showing another biotech applicability for the exopolysaccharides produced by C. brachyspora .
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معلومات مُعتمدة: 001 Fundação Araucária; CP 08/2009 - PP-SUS Ministry of Health
فهرسة مساهمة: Keywords: biological activity; dematiaceous fungi; fungal production; polysaccharide; response surface methodology
المشرفين على المادة: 0 (Polysaccharides)
SCR Organism: Curvularia brachyspora
تواريخ الأحداث: Date Created: 20230610 Date Completed: 20230612 Latest Revision: 20230612
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10254318
DOI: 10.3390/molecules28114356
PMID: 37298832
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules28114356