دورية أكاديمية
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Species diversity and molecular characterization of Alternaria section Alternaria isolates collected mainly from cereal crops in Canada.

التفاصيل البيبلوغرافية
العنوان: Species diversity and molecular characterization of Alternaria section Alternaria isolates collected mainly from cereal crops in Canada.
المؤلفون: Dettman JR; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada., Eggertson QA; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada., Kim NE; Agriculture and Agri-Food Canada, Ottawa Research and Development Centre, Ottawa, ON, Canada.
المصدر: Frontiers in microbiology [Front Microbiol] 2023 May 25; Vol. 14, pp. 1194911. Date of Electronic Publication: 2023 May 25 (Print Publication: 2023).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Frontiers Research Foundation Country of Publication: Switzerland NLM ID: 101548977 Publication Model: eCollection Cited Medium: Print ISSN: 1664-302X (Print) Linking ISSN: 1664302X NLM ISO Abbreviation: Front Microbiol Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Lausanne : Frontiers Research Foundation
مستخلص: Alternaria is often one on the most abundant fungal genera recovered from a wide array of plant hosts and environmental substrates. Many species within the sub-generic Alternaria section Alternaria are common plant pathogens that cause pre-harvest losses due to reduced productivity and post-harvest losses due to spoilage and contamination with mycotoxins. As certain species of Alternaria may have distinct mycotoxin profiles, and very broad host ranges, understanding the distribution of species by geography and host is critical for disease prediction, toxicological risk assessment, and guiding regulatory decisions. In two previous reports, we performed phylogenomic analyses to identify highly informative molecular markers for Alternaria section Alternaria , and validated their diagnostic ability. Here, we perform molecular characterization of 558 section Alternaria strains, collected from 64 host genera in 12 countries, using two of these section-specific loci ( ASA-10 and ASA-19 ) along with the RNA polymerase II second largest subunit ( rpb2 ) gene. The majority of strains (57.4%) originated from various cereal crops in Canada, which formed the main focus of our study. Phylogenetic analyses were used to classify strains into section Alternaria species/lineages, demonstrating that the most common species on Canadian cereal crops are Alternaria alternata and A. arborescens. Further population genetic analyses were consistent with A. alternata being a widely distributed species with relatively low levels of geographic isolation (i.e., Canadian isolates did not form distinct clades when compared to other regions). Our expanded sampling of A. arborescens has greatly increased the known diversity of this group, with A. arborescens isolates forming at least three distinct phylogenetic lineages. Proportionally, A. arborescens is more prevalent in Eastern Canada than in Western Canada. Sequence analyses, putative hybrids, and mating-type distributions provided some evidence for recombination events, both within and between species. There was little evidence for associations between hosts and genetic haplotypes of A. alternata or A. arborescens .
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2023 His Majesty the King in Right of Canada, as represented by the Minister of Agriculture and Agri-Food Canada for the contribution of Jeremy R. Dettman, Quinn Eggertson, and Natalie E. Kim.)
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فهرسة مساهمة: Keywords: A. arborescens; ASA-10; ASA-19; Alternaria alternata; RPB2; species complex
تواريخ الأحداث: Date Created: 20230612 Latest Revision: 20230613
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10249498
DOI: 10.3389/fmicb.2023.1194911
PMID: 37303811
قاعدة البيانات: MEDLINE
الوصف
تدمد:1664-302X
DOI:10.3389/fmicb.2023.1194911