دورية أكاديمية
أعرض في EDS

Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study.

التفاصيل البيبلوغرافية
العنوان: Host defense peptides combined with MTA extract increase the repair in dental pulp cells: in vitro and ex vivo study.
المؤلفون: Silva PAO; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil., Martins DCM; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil., de Castro Cantuária AP; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil., de Andrade RV; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, Room C-22170.790-160, Brasília, Distrito Federal, Brazil., Lacorte C; Laboratório de Biologia Sintética, Embrapa Recursos Genéticos e Biotecnologia, Brasília, Distrito Federal, Brazil., de Almeida JA; Curso de Educação Física, Universidade Federal de Mato Grosso do Sul, UFMS, Campo Grande, Mato Grosso do Sul, Brazil., Aguiar LR; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, Room C-22170.790-160, Brasília, Distrito Federal, Brazil., Corrêa JR; Laboratório de Microscopia e Microanálises, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, Brazil., da Silva IGM; Laboratório de Microscopia e Microanálises, Instituto de Ciências Biológicas, Universidade de Brasília, Brasília, Distrito Federal, Brazil., Franco OL; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, Room C-22170.790-160, Brasília, Distrito Federal, Brazil.; S-Inova Biotech, Pós-Graduação em Biotecnologia, Universidade Católica Dom Bosco, Campo Grande, Mato Grosso do Sul, Brazil., Rezende TMB; Programa de Pós-Graduação em Ciências da Saúde, Universidade de Brasília, Brasília, Distrito Federal, Brazil. taiambr@gmail.com.; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, SGAN 916N - Av. W5 - Campus II - Modulo C, Room C-22170.790-160, Brasília, Distrito Federal, Brazil. taiambr@gmail.com.; Curso de Odontologia, Universidade de Brasília, Brasília, Distrito Federal, Brazil. taiambr@gmail.com.
المصدر: Scientific reports [Sci Rep] 2023 Jun 12; Vol. 13 (1), pp. 9531. Date of Electronic Publication: 2023 Jun 12.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Dental Pulp* , Calcinosis*, Humans ; Interleukin-6 ; Antimicrobial Cationic Peptides ; Alkaline Phosphatase ; Analysis of Variance
مستخلص: Host Defense Peptides (HDPs) have, in previous studies, been demonstrating antimicrobial, anti-inflammatory, and immunomodulatory capacity, important factors in the repair process. Knowing these characteristics, this article aims to evaluate the potential of HDPs IDR1018 and DJK-6 associated with MTA extract in the repair process of human pulp cells. Antibacterial activity of HDPs, MTA and HDPs combined with MTA in Streptococcus mutans planktonic bacteria and antibiofilm activity was evaluated. Cell toxicity was assayed with MTT and cell morphology was observed by scanning electron microscopy (SEM). Proliferation and migration of pulp cells were evaluated by trypan blue and wound healing assay. Inflammatory and mineralization related genes were evaluated by qPCR (IL-6, TNFRSF, DSPP, TGF-β). Alkaline phosphatase, phosphate quantification and alizarin red staining were also verified. The assays were performed in technical and biological triplicate (n = 9). Results were submitted for the calculation of the mean and standard deviation. Then, normality verification by Kolmogorov Smirnov test, analyzing one-way ANOVA. Analyses were considered at a 95% significance level, with a p-value < 0.05. Our study demonstrated that HDPs combined with MTA were able to reduce biofilms performed in 24 h and biofilm performed over 7 days S. mutans biofilm (p < 0.05). IDR1018 and MTA, as well as their combination, down-regulated IL-6 expression (p < 0.05). Tested materials were not cytotoxic to pulp cells. IDR1018 induced high cell proliferation and combined with MTA induced high cellular migration rates in 48 h (p < 0.05). Furthermore, the combination of IDR1018 and MTA also induced high expression levels of DSPP, ALP activity, and the production of calcification nodules. So, IDR-1018 and its combination with MTA could assist in pulp-dentine complex repair process in vitro.
(© 2023. The Author(s).)
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المشرفين على المادة: 0 (Interleukin-6)
0 (Antimicrobial Cationic Peptides)
EC 3.1.3.1 (Alkaline Phosphatase)
تواريخ الأحداث: Date Created: 20230612 Date Completed: 20230614 Latest Revision: 20230628
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10261146
DOI: 10.1038/s41598-023-36748-3
PMID: 37308525
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-36748-3