دورية أكاديمية
أعرض في EDS

The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications.

التفاصيل البيبلوغرافية
العنوان: The cofactor challenge in synthetic methylotrophy: bioengineering and industrial applications.
المؤلفون: Krüsemann JL; Charité - Universitätsmedizin Berlin, Department of Biochemistry, Charitéplatz 1, 10117 Berlin, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany; Max Planck Institute for Terrestrial Microbiology, Department of Biochemistry and Synthetic Metabolism, Karl-von-Frisch-Str. 10, 35043 Marburg, Germany., Rainaldi V; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands., Cotton CA; Cambrium GmbH, Max-Urich-Straße 3, 13355 Berlin, Germany., Claassens NJ; Laboratory of Microbiology, Wageningen University, Stippeneng 4, 6708 WE Wageningen, The Netherlands., Lindner SN; Charité - Universitätsmedizin Berlin, Department of Biochemistry, Charitéplatz 1, 10117 Berlin, Germany; Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam, Germany. Electronic address: steffen.lindner@charite.de.
المصدر: Current opinion in biotechnology [Curr Opin Biotechnol] 2023 Aug; Vol. 82, pp. 102953. Date of Electronic Publication: 2023 Jun 13.
نوع المنشور: Journal Article; Review; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Country of Publication: England NLM ID: 9100492 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0429 (Electronic) Linking ISSN: 09581669 NLM ISO Abbreviation: Curr Opin Biotechnol Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Elsevier
Original Publication: London : Current Biology, c1990-
مواضيع طبية MeSH: NAD* , Methanol*, Hydrogen Peroxide ; PQQ Cofactor ; Bioengineering
مستخلص: Methanol is a promising feedstock for industrial bioproduction: it can be produced renewably and has high solubility and limited microbial toxicity. One of the key challenges for its bio-industrial application is the first enzymatic oxidation step to formaldehyde. This reaction is catalysed by methanol dehydrogenases (MDH) that can use NAD + , O 2 or pyrroloquinoline quinone (PQQ) as an electron acceptor. While NAD-dependent MDH are simple to express and have the highest energetic efficiency, they exhibit mediocre kinetics and poor thermodynamics at ambient temperatures. O 2 -dependent methanol oxidases require high oxygen concentrations, do not conserve energy and thus produce excessive heat as well as toxic H 2 O 2 . PQQ-dependent MDH provide a good compromise between energy efficiency and good kinetics that support fast growth rates without any drawbacks for process engineering. Therefore, we argue that this enzyme class represents a promising solution for industry and outline engineering strategies for the implementation of these complex systems in heterologous hosts.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
المشرفين على المادة: 0U46U6E8UK (NAD)
Y4S76JWI15 (Methanol)
BBX060AN9V (Hydrogen Peroxide)
72909-34-3 (PQQ Cofactor)
تواريخ الأحداث: Date Created: 20230615 Date Completed: 20230802 Latest Revision: 20230806
رمز التحديث: 20231215
DOI: 10.1016/j.copbio.2023.102953
PMID: 37320962
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0429
DOI:10.1016/j.copbio.2023.102953