دورية أكاديمية
أعرض في EDS

Discovery of a new Hepatozoon species namely Hepatozoon viperoi sp. nov. in nose-horned vipers in Türkiye.

التفاصيل البيبلوغرافية
العنوان: Discovery of a new Hepatozoon species namely Hepatozoon viperoi sp. nov. in nose-horned vipers in Türkiye.
المؤلفون: Ceylan O; Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye. onurceylan@selcuk.edu.tr., Úngari LP; Instituto de Biociências, Campus de Botucatu, Departamento de Parasitologia, UNESP-Univ. Estadual Paulista, Distrito de Rubião Junior, Botucatu, SP, 18618-689, Brazil., Sönmez G; Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye., Gul C; Department of Biology, Faculty of Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., Ceylan C; Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, 56100, Siirt, Türkiye., Tosunoglu M; Department of Biology, Faculty of Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., Baycan B; Department of Biology, School of Graduate Studies, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., O'Dwyer LH; Instituto de Biociências, Campus de Botucatu, Departamento de Parasitologia, UNESP-Univ. Estadual Paulista, Distrito de Rubião Junior, Botucatu, SP, 18618-689, Brazil., Sevinc F; Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye.
المصدر: Scientific reports [Sci Rep] 2023 Jun 15; Vol. 13 (1), pp. 9677. Date of Electronic Publication: 2023 Jun 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Eucoccidiida*/genetics , Colubridae* , Viperidae*/genetics , Coccidiosis*/veterinary , Coccidiosis*/parasitology, Animals ; Phylogeny ; RNA, Ribosomal, 18S/genetics
مستخلص: Although Hepatozoon spp. remains the most prevalent intracellular protozoa infecting snakes, it was reported only in a few snake species of the Colubridae family in Türkiye. Moreover, studies on these hemoparasites are not available in venomous nose-horned vipers from Türkiye. In this study, we investigated Hepatozoon spp. in three individual Vipera ammodytes using morphological and molecular methods. Our results were positive for intraerythrocytic Hepatozoon spp. gamonts in all three snakes, exhibiting low parasitemia. The microscopic findings were further confirmed through molecular data. A genus-specific PCR assay targeting the 18S rRNA gene region of Hepatozoon spp., was performed using HemoF/HemoR and Hep300/Hep900 primers. The obtained sequences were concatenated and used in phylogenetic analyses in comparison with different Hepatozoon species. Although our (OP377741) isolate was separated into a different branch, it was clustered with the isolates of H. massardi (KC342526), H. cevapii (KC342525), and H. annulatum (ON262426) from Brazilian snakes. Moreover, gene similarity and pair-wise distance between our isolate and other Hepatozoon species infecting snakes were found to be 89.30-98.63% and 0.009-0.077, respectively. Hence, we reported a new species of Hepatozoon, namely Hepatozoon viperoi sp. nov. infecting V. ammodytes. Since the literature does not indicate the existence of such a Hepatozoon species in V. ammodytes in different countries, our data may contribute to the expanding knowledge of Hepatozoon species in snakes, providing new insights into the biodiversity of the haemogregarine protozoan parasite.
(© 2023. The Author(s).)
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المشرفين على المادة: 0 (RNA, Ribosomal, 18S)
تواريخ الأحداث: Date Created: 20230615 Date Completed: 20230619 Latest Revision: 20231121
رمز التحديث: 20231122
مُعرف محوري في PubMed: PMC10272196
DOI: 10.1038/s41598-023-36814-w
PMID: 37322098
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-36814-w