دورية أكاديمية
Discovery of a new Hepatozoon species namely Hepatozoon viperoi sp. nov. in nose-horned vipers in Türkiye.
العنوان: | Discovery of a new Hepatozoon species namely Hepatozoon viperoi sp. nov. in nose-horned vipers in Türkiye. |
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المؤلفون: | Ceylan O; Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye. onurceylan@selcuk.edu.tr., Úngari LP; Instituto de Biociências, Campus de Botucatu, Departamento de Parasitologia, UNESP-Univ. Estadual Paulista, Distrito de Rubião Junior, Botucatu, SP, 18618-689, Brazil., Sönmez G; Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye., Gul C; Department of Biology, Faculty of Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., Ceylan C; Department of Parasitology, Faculty of Veterinary Medicine, Siirt University, 56100, Siirt, Türkiye., Tosunoglu M; Department of Biology, Faculty of Sciences, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., Baycan B; Department of Biology, School of Graduate Studies, Çanakkale Onsekiz Mart University, Çanakkale, Türkiye., O'Dwyer LH; Instituto de Biociências, Campus de Botucatu, Departamento de Parasitologia, UNESP-Univ. Estadual Paulista, Distrito de Rubião Junior, Botucatu, SP, 18618-689, Brazil., Sevinc F; Department of Veterinary Parasitology, Faculty of Veterinary Medicine, Selcuk University, 42130, Konya, Türkiye. |
المصدر: | Scientific reports [Sci Rep] 2023 Jun 15; Vol. 13 (1), pp. 9677. Date of Electronic Publication: 2023 Jun 15. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: London : Nature Publishing Group, copyright 2011- |
مواضيع طبية MeSH: | Eucoccidiida*/genetics , Colubridae* , Viperidae*/genetics , Coccidiosis*/veterinary , Coccidiosis*/parasitology, Animals ; Phylogeny ; RNA, Ribosomal, 18S/genetics |
مستخلص: | Although Hepatozoon spp. remains the most prevalent intracellular protozoa infecting snakes, it was reported only in a few snake species of the Colubridae family in Türkiye. Moreover, studies on these hemoparasites are not available in venomous nose-horned vipers from Türkiye. In this study, we investigated Hepatozoon spp. in three individual Vipera ammodytes using morphological and molecular methods. Our results were positive for intraerythrocytic Hepatozoon spp. gamonts in all three snakes, exhibiting low parasitemia. The microscopic findings were further confirmed through molecular data. A genus-specific PCR assay targeting the 18S rRNA gene region of Hepatozoon spp., was performed using HemoF/HemoR and Hep300/Hep900 primers. The obtained sequences were concatenated and used in phylogenetic analyses in comparison with different Hepatozoon species. Although our (OP377741) isolate was separated into a different branch, it was clustered with the isolates of H. massardi (KC342526), H. cevapii (KC342525), and H. annulatum (ON262426) from Brazilian snakes. Moreover, gene similarity and pair-wise distance between our isolate and other Hepatozoon species infecting snakes were found to be 89.30-98.63% and 0.009-0.077, respectively. Hence, we reported a new species of Hepatozoon, namely Hepatozoon viperoi sp. nov. infecting V. ammodytes. Since the literature does not indicate the existence of such a Hepatozoon species in V. ammodytes in different countries, our data may contribute to the expanding knowledge of Hepatozoon species in snakes, providing new insights into the biodiversity of the haemogregarine protozoan parasite. (© 2023. The Author(s).) |
References: | PLoS Negl Trop Dis. 2014 Jan 30;8(1):e2668. (PMID: 24498452) Acta Parasitol. 2021 Sep;66(3):871-880. (PMID: 33683553) Nat Methods. 2012 Jul 30;9(8):772. (PMID: 22847109) J Parasitol. 2012 Oct;98(5):913-7. (PMID: 22551400) Syst Parasitol. 2014 Mar;87(3):249-58. (PMID: 24563141) J Parasitol. 2001 Aug;87(4):870-6. (PMID: 11534653) Bioinformatics. 2014 May 1;30(9):1312-3. (PMID: 24451623) Exp Parasitol. 2014 Dec;147:7-15. (PMID: 25300762) Vet Parasitol. 2011 Sep 8;181(1):3-11. (PMID: 21620568) Syst Biol. 2003 Oct;52(5):696-704. (PMID: 14530136) Parasitol Int. 2022 Aug;89:102587. (PMID: 35452797) Animals (Basel). 2021 Jan 12;11(1):. (PMID: 33445814) Parasitology. 2014 Mar;141(3):389-98. (PMID: 24553081) Exp Parasitol. 2013 Oct;135(2):200-7. (PMID: 23867148) Ecohealth. 2016 Sep;13(3):467-479. (PMID: 27384647) Parasite. 2020;27:73. (PMID: 33332263) Trends Parasitol. 2009 Aug;25(8):375-82. (PMID: 19635681) Parasitol Res. 2012 Apr;110(4):1393-401. (PMID: 21922238) J Parasitol. 1996 Aug;82(4):565-85. (PMID: 8691364) Pathogens. 2021 Feb 19;10(2):. (PMID: 33669573) Bioinformatics. 2001 Aug;17(8):754-5. (PMID: 11524383) Folia Parasitol (Praha). 2018 Apr 3;65:. (PMID: 29657154) Vet Clin North Am Exot Anim Pract. 2013 Jan;16(1):1-30. (PMID: 23347537) Curr Protoc Bioinformatics. 2003 Feb;Chapter 6:Unit 6.5. (PMID: 18428705) J Parasitol. 2004 Jun;90(3):670-2. (PMID: 15270125) Parasitol Res. 2020 Nov;119(11):3793-3801. (PMID: 32979103) Int J Parasitol Parasites Wildl. 2017 Aug 26;6(3):241-256. (PMID: 28913164) J Comp Physiol B. 2012 Jan;182(1):139-55. (PMID: 21710385) Bioinformatics. 2012 Jun 15;28(12):1647-9. (PMID: 22543367) Parasitol Res. 2018 Dec;117(12):3857-3865. (PMID: 30267181) Parasitol Res. 1991;77(5):402-10. (PMID: 1891449) Parasitol Res. 2013 Jul;112(7):2533-42. (PMID: 23620221) Vet Microbiol. 2016;182:35-43. (PMID: 26711026) Syst Parasitol. 2017 Jan;94(1):65-72. (PMID: 28062992) |
المشرفين على المادة: | 0 (RNA, Ribosomal, 18S) |
تواريخ الأحداث: | Date Created: 20230615 Date Completed: 20230619 Latest Revision: 20231121 |
رمز التحديث: | 20231122 |
مُعرف محوري في PubMed: | PMC10272196 |
DOI: | 10.1038/s41598-023-36814-w |
PMID: | 37322098 |
قاعدة البيانات: | MEDLINE |
تدمد: | 2045-2322 |
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DOI: | 10.1038/s41598-023-36814-w |