Functional differences in agonist-induced plasma membrane expression of Orai1α and Orai1β.

التفاصيل البيبلوغرافية
العنوان:	Functional differences in agonist-induced plasma membrane expression of Orai1α and Orai1β.
المؤلفون:	Jardin I; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Alvarado S; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Sanchez-Collado J; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Nieto-Felipe J; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Lopez JJ; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Salido GM; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain., Rosado JA; Department of Physiology (Cellular Physiology Research Group), Institute of Molecular Pathology Biomarkers (IMPB), University of Extremadura, Caceres, Spain.
المصدر:	Journal of cellular physiology [J Cell Physiol] 2023 Sep; Vol. 238 (9), pp. 2050-2062. Date of Electronic Publication: 2023 Jun 18.
نوع المنشور:	Journal Article; Research Support, Non-U.S. Gov't
اللغة:	English
بيانات الدورية:	Publisher: Wiley-Liss Country of Publication: United States NLM ID: 0050222 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4652 (Electronic) Linking ISSN: 00219541 NLM ISO Abbreviation: J Cell Physiol Subsets: MEDLINE
أسماء مطبوعة:	Publication: New York, NY : Wiley-Liss Original Publication: Philadelphia, Wistar Institute of Anatomy and Biology.
مواضيع طبية MeSH:	Calcium Channels/genetics , Calcium Channels/metabolism , Calcium Release Activated Calcium Channels/metabolism , ORAI1 Protein/antagonists & inhibitors , ORAI1 Protein/genetics , ORAI1 Protein/metabolism, Calcium/metabolism ; Calcium Signaling ; Cell Membrane/metabolism ; Stromal Interaction Molecule 1/metabolism ; Thapsigargin/pharmacology ; Humans ; HEK293 Cells
مستخلص:	Orai1 is the pore-forming subunit of the store-operated Ca ²⁺ release-activated Ca ²⁺ (CRAC) channels involved in a variety of cellular functions. Two Orai1 variants have been identified, the long form, Orai1α, containing 301 amino acids, and the short form, Orai1β, which arises from alternative translation initiation from methionines 64 or 71, in Orai1α. Orai1 is mostly expressed in the plasma membrane, but a subset of Orai1 is located in intracellular compartments. Here we show that Ca ²⁺ store depletion leads to trafficking and insertion of compartmentalized Orai1α in the plasma membrane via a mechanism that is independent on changes in cytosolic free-Ca ²⁺ concentration, as demonstrated by cell loading with the fast intracellular Ca ²⁺ chelator dimethyl BAPTA in the absence of extracellular Ca ²⁺ . Interestingly, thapsigargin (TG) was found to be unable to induce translocation of Orai1β to the plasma membrane when expressed individually; by contrast, when Orai1β is co-expressed with Orai1α, cell treatment with TG induced rapid trafficking and insertion of compartmentalized Orai1β in the plasma membrane. Translocation of Orai1 forms to the plasma membrane was found to require the integrity of the actin cytoskeleton. Finally, expression of a dominant negative mutant of the small GTPase ARF6, and ARF6-T27N, abolished the translocation of compartmentalized Orai1 variants to the plasma membrane upon store depletion. These findings provide new insights into the mechanism that regulate the plasma membrane abundance of Orai1 variants after Ca ²⁺ store depletion. (© 2023 The Authors. Journal of Cellular Physiology published by Wiley Periodicals LLC.)
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فهرسة مساهمة:	Keywords: ARF6; Orai1α; Orai1β; actin cytoskeleton; plasma membrane; store-operated Ca2+ entry
المشرفين على المادة:	SY7Q814VUP (Calcium) 0 (Calcium Channels) 0 (Calcium Release Activated Calcium Channels) 0 (ORAI1 Protein) 0 (Stromal Interaction Molecule 1) 67526-95-8 (Thapsigargin)
تواريخ الأحداث:	Date Created: 20230619 Date Completed: 20230925 Latest Revision: 20230925
رمز التحديث:	20230926
DOI:	10.1002/jcp.31055
PMID:	37332264
قاعدة البيانات:	MEDLINE

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تدمد:	1097-4652
DOI:	10.1002/jcp.31055