التفاصيل البيبلوغرافية
| العنوان: |
High-throughput confocal airy beam oblique light-sheet tomography of brain-wide imaging at single-cell resolution. |
| المؤلفون: |
Qi X, Muñoz-Castañeda R, Narasimhan A, Ding L, Chen X, Elowsky C, Palmer J, Drewes R, Sun J, Mizrachi J, Peng H, Wu Z, Osten P |
| المصدر: |
BioRxiv : the preprint server for biology [bioRxiv] 2023 Jun 06. Date of Electronic Publication: 2023 Jun 06. |
| نوع المنشور: |
Preprint |
| اللغة: |
English |
| بيانات الدورية: |
Country of Publication: United States NLM ID: 101680187 Publication Model: Electronic Cited Medium: Internet NLM ISO Abbreviation: bioRxiv Subsets: PubMed not MEDLINE |
| مستخلص: |
Brain research is an area of research characterized by its cutting-edge nature, with brain mapping constituting a crucial aspect of this field. As sequencing tools have played a crucial role in gene sequencing, brain mapping largely depends on automated, high-throughput and high-resolution imaging techniques. Over the years, the demand for high-throughput imaging has scaled exponentially with the rapid development of microscopic brain mapping. In this paper, we introduce the novel concept of confocal Airy beam into oblique light-sheet tomography named CAB-OLST. We demonstrate that this technique enables the high throughput of brain-wide imaging of long-distance axon projection for the entire mouse brain at a resolution of 0.26 μm × 0.26 μm × 1.06 μm in 58 hours. This technique represents an innovative contribution to the field of brain research by setting a new standard for high-throughput imaging techniques. |
| معلومات مُعتمدة: |
U01 MH114824 United States MH NIMH NIH HHS |
| تواريخ الأحداث: |
Date Created: 20230619 Latest Revision: 20230916 |
| رمز التحديث: |
20231215 |
| مُعرف محوري في PubMed: |
PMC10274617 |
| DOI: |
10.1101/2023.06.04.543586 |
| PMID: |
37333143 |
| قاعدة البيانات: |
MEDLINE |
