دورية أكاديمية
Eastern Equine Encephalitis Virus Diversity in Massachusetts Patients, 1938-2020.
| العنوان: | Eastern Equine Encephalitis Virus Diversity in Massachusetts Patients, 1938-2020. |
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| المؤلفون: | Langsjoen RM; Department of Pathology, Emory University School of Medicine, Atlanta, Georgia., Key A; Department of Pathology, Emory University School of Medicine, Atlanta, Georgia., Shariatzadeh N; Department of Pathology, Emory University School of Medicine, Atlanta, Georgia., Jackson CR; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts., Mahmood F; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts., Arkun K; Department of Pathology and Laboratory Medicine, Tufts Medical Center, Boston, Massachusetts., Alexandrescu S; Department of Pathology, Boston Children's Hospital, Harvard Medical School, Boston, Massachusetts., Solomon IH; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts., Piantadosi A; Department of Pathology, Emory University School of Medicine, Atlanta, Georgia.; Division of Infectious Diseases, Department of Medicine, Emory University School of Medicine, Atlanta, Georgia. |
| المصدر: | The American journal of tropical medicine and hygiene [Am J Trop Med Hyg] 2023 Jun 20; Vol. 109 (2), pp. 387-396. Date of Electronic Publication: 2023 Jun 20 (Print Publication: 2023). |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society of Tropical Medicine and Hygiene Country of Publication: United States NLM ID: 0370507 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1476-1645 (Electronic) Linking ISSN: 00029637 NLM ISO Abbreviation: Am J Trop Med Hyg Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Northbrook, IL : American Society of Tropical Medicine and Hygiene Original Publication: Baltimore. |
| مواضيع طبية MeSH: | Encephalitis Virus, Eastern Equine*/genetics , Encephalomyelitis, Equine*/epidemiology, Humans ; Animals ; Horses/genetics ; Phylogeny ; Massachusetts/epidemiology ; RNA, Viral/genetics |
| مستخلص: | Eastern equine encephalitis virus (EEEV) is a relatively little-studied alphavirus that can cause devastating viral encephalitis, potentially leading to severe neurological sequelae or death. Although case numbers have historically been low, outbreaks have been increasing in frequency and scale since the 2000 s. It is critical to investigate EEEV evolutionary patterns, especially within human hosts, to understand patterns of emergence, host adaptation, and within-host evolution. To this end, we obtained formalin-fixed paraffin-embedded tissue blocks from discrete brain regions from five contemporary (2004-2020) patients from Massachusetts, confirmed the presence of EEEV RNA by in situ hybridization (ISH) staining, and sequenced viral genomes. We additionally sequenced RNA from scrapings of historical slides made from brain sections of a patient in the first documented EEE outbreak in humans in 1938. ISH staining revealed the presence of RNA in all contemporary samples, and quantification loosely correlated with the proportion of EEEV reads in samples. Consensus EEEV sequences were generated for all six patients, including the sample from 1938; phylogenetic analysis using additional publicly available sequences revealed clustering of each study sample with like sequences from a similar region, whereas an intrahost comparison of consensus sequences between discrete brain regions revealed minimal changes. Intrahost single nucleotide variant (iSNV) analysis of four samples from two patients revealed the presence of tightly compartmentalized, mostly nonsynonymous iSNVs. This study contributes critical primary human EEEV sequences, including a historic sequence as well as novel intrahost evolution findings, contributing substantially to our understanding of the natural history of EEEV infection in humans. |
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| معلومات مُعتمدة: | P30 CA006516 United States CA NCI NIH HHS; R21 NS119660 United States NS NINDS NIH HHS |
| المشرفين على المادة: | 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20230620 Date Completed: 20230804 Latest Revision: 20231020 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10397450 |
| DOI: | 10.4269/ajtmh.23-0047 |
| PMID: | 37339758 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1476-1645 |
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| DOI: | 10.4269/ajtmh.23-0047 |