دورية أكاديمية
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Characterization of two novel lytic bacteriophages having lysis potential against MDR avian pathogenic Escherichia coli strains of zoonotic potential.

التفاصيل البيبلوغرافية
العنوان: Characterization of two novel lytic bacteriophages having lysis potential against MDR avian pathogenic Escherichia coli strains of zoonotic potential.
المؤلفون: Sattar S; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan. sadia.sattar@comsats.edu.pk., Bailie M; AgResearch, Palmerston North, 4410, New Zealand., Yaqoob A; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Khanum S; AgResearch, Palmerston North, 4410, New Zealand., Fatima K; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Altaf AURB; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Ahmed I; Alpha Genomics Private Limited, Islamabad, 45710, Pakistan., Shah STA; Functional Genomics Lab, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Munawar J; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Zehra QA; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Daud S; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Arshad A; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Imdad K; Microbiology and Immunology Lab, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Javed S; Microbiology and Immunology Lab, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Tariq A; Microbiology and Immunology Lab, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Bostan N; Molecular Virology Labs, Department of Biosciences, Comsats University Islamabad, Islamabad, 45550, Pakistan., Altermann E; School of Veterinary Science Massey University Centre for Bioparticle Applications, Massey University, Palmerston North, 4472, New Zealand.
المصدر: Scientific reports [Sci Rep] 2023 Jun 20; Vol. 13 (1), pp. 10043. Date of Electronic Publication: 2023 Jun 20.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Escherichia coli Infections*/prevention & control , Escherichia coli Infections*/veterinary , Escherichia coli Infections*/microbiology , Bacteriophages*/genetics , Poultry Diseases*/prevention & control , Poultry Diseases*/microbiology, Animals ; Humans ; Escherichia coli/genetics ; Birds/microbiology ; Poultry ; Chickens
مستخلص: Avian pathogenic E. coli (APEC) is associated with local and systemic infections in poultry, ducks, turkeys, and many other avian species, leading to heavy economical losses. These APEC strains are presumed to possess zoonotic potential due to common virulence markers that can cause urinary tract infections in humans. The prophylactic use of antibiotics in the poultry sector has led to the rapid emergence of Multiple Drug Resistant (MDR) APEC strains that act as reservoirs and put human populations at risk. This calls for consideration of alternative strategies to decrease the bacterial load. Here, we report isolation, preliminary characterization, and genome analysis of two novel lytic phage species (Escherichia phage SKA49 and Escherichia phage SKA64) against MDR strain of APEC, QZJM25. Both phages were able to keep QZJM25 growth significantly less than the untreated bacterial control for approximately 18 h. The host range was tested against Escherichia coli strains of poultry and human UTI infections. SKA49 had a broader host range in contrast to SKA64. Both phages were stable at 37 °C only. Their genome analysis indicated their safety as no recombination, integration and host virulence genes were identified. Both these phages can be good candidates for control of APEC strains based on their lysis potential.
(© 2023. The Author(s).)
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تواريخ الأحداث: Date Created: 20230620 Date Completed: 20230622 Latest Revision: 20230701
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC10282059
DOI: 10.1038/s41598-023-37176-z
PMID: 37340022
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-023-37176-z