دورية أكاديمية
أعرض في EDS

A local-scale One Health genomic surveillance of Clostridioides difficile demonstrates highly related strains from humans, canines, and the environment.

التفاصيل البيبلوغرافية
العنوان: A local-scale One Health genomic surveillance of Clostridioides difficile demonstrates highly related strains from humans, canines, and the environment.
المؤلفون: Williamson CHD; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Roe CC; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Terriquez J; Flagstaff Medical Center, Flagstaff, AZ, USA., Hornstra H; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Lucero S; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Nunnally AE; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Vazquez AJ; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Vinocur J; Flagstaff Medical Center, Flagstaff, AZ, USA., Plude C; Flagstaff Medical Center, Flagstaff, AZ, USA., Nienstadt L; Flagstaff Medical Center, Flagstaff, AZ, USA., Stone NE; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Celona KR; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Wagner DM; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Keim P; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA., Sahl JW; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ, USA.
المصدر: Microbial genomics [Microb Genom] 2023 Jun; Vol. 9 (6).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Microbiology Society Country of Publication: England NLM ID: 101671820 Publication Model: Print Cited Medium: Internet ISSN: 2057-5858 (Electronic) Linking ISSN: 20575858 NLM ISO Abbreviation: Microb Genom Subsets: MEDLINE
أسماء مطبوعة: Publication: Oct. 2015- : [London] : Microbiology Society
Original Publication: [London] : Society for General Microbiology, [2015]-
مواضيع طبية MeSH: Clostridioides difficile* , Bacterial Toxins*/genetics , One Health* , Clostridium Infections*/epidemiology , Clostridium Infections*/veterinary, Humans ; Animals ; Dogs ; Clostridioides ; Genomics
مستخلص: Although infections caused by Clostridioides difficile have historically been attributed to hospital acquisition, growing evidence supports the role of community acquisition in C. difficile infection (CDI). Symptoms of CDI can range from mild, self-resolving diarrhoea to toxic megacolon, pseudomembranous colitis, and death. In this study, we sampled C. difficile from clinical, environmental, and canine reservoirs in Flagstaff, Arizona, USA, to understand the distribution and transmission of the pathogen in a One Health framework; Flagstaff is a medium-sized, geographically isolated city with a single hospital system, making it an ideal site to characterize genomic overlap between sequenced C. difficile isolates across reservoirs. An analysis of 562 genomes from Flagstaff isolates identified 65 sequence types (STs), with eight STs being found across all three reservoirs and another nine found across two reservoirs. A screen of toxin genes in the pathogenicity locus identified nine STs where all isolates lost the toxin genes needed for CDI manifestation ( tcdB , tcdA ), demonstrating the widespread distribution of non-toxigenic C. difficile (NTCD) isolates in all three reservoirs; 15 NTCD genomes were sequenced from symptomatic, clinical samples, including two from mixed infections that contained both tcdB+ and tcdB - isolates. A comparative single nucleotide polymorphism (SNP) analysis of clinically derived isolates identified 78 genomes falling within clusters separated by ≤2 SNPs, indicating that ~19 % of clinical isolates are associated with potential healthcare-associated transmission clusters; only symptomatic cases were sampled in this study, and we did not sample asymptomatic transmission. Using this same SNP threshold, we identified genomic overlap between canine and soil isolates, as well as putative transmission between environmental and human reservoirs. The core genome of isolates sequenced in this study plus a representative set of public C. difficile genomes ( n =136), was 2690 coding region sequences, which constitutes ~70 % of an individual C. difficile genome; this number is significantly higher than has been published in some other studies, suggesting that genome data quality is important in understanding the minimal number of genes needed by C. difficile . This study demonstrates the close genomic overlap among isolates sampled across reservoirs, which was facilitated by maximizing the genomic search space used for comprehensive identification of potential transmission events. Understanding the distribution of toxigenic and non-toxigenic C. difficile across reservoirs has implications for surveillance sampling strategies, characterizing routes of infections, and implementing mitigation measures to limit human infection.
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فهرسة مساهمة: Keywords: Clostridioides difficileinfection; One Health; comparative genomics
المشرفين على المادة: 0 (trimethylaminocarboxyldihydroboran)
0 (Bacterial Toxins)
تواريخ الأحداث: Date Created: 20230622 Date Completed: 20230626 Latest Revision: 20231116
رمز التحديث: 20240628
مُعرف محوري في PubMed: PMC10327504
DOI: 10.1099/mgen.0.001046
PMID: 37347682
قاعدة البيانات: MEDLINE
الوصف
تدمد:2057-5858
DOI:10.1099/mgen.0.001046