دورية أكاديمية
In situ characterization of the agglutination of lectins via cross-linking of carbohydrates by time-resolved measurement of forward static light scattering.
| العنوان: | In situ characterization of the agglutination of lectins via cross-linking of carbohydrates by time-resolved measurement of forward static light scattering. |
|---|---|
| المؤلفون: | Ogata M; Faculty of Food and Agricultural Sciences, Fukushima University, 1 Kanayagawa, Fukushima city, Fukushima, Japan.; Department of Applied Chemistry and Biochemistry, National Institute of Technology, Fukushima College, 30 Nagao, Iwaki, Fukushima, Japan., Onoda T; Department of Applied Chemistry and Biochemistry, National Institute of Technology, Fukushima College, 30 Nagao, Iwaki, Fukushima, Japan., Wakamatsu T; Department of Electrical and Electronic System Engineering, National Institute of Technology, Fukushima College, 30 Nagao, Iwaki, Fukushima, Japan.; Department of Industrial Engineering, National Institute of Technology, Ibaraki College, 866 Nakane, Hitachinaka, Ibaraki, Japan. |
| المصدر: | Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2023 Aug 23; Vol. 87 (9), pp. 1036-1044. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 9205717 Publication Model: Print Cited Medium: Internet ISSN: 1347-6947 (Electronic) Linking ISSN: 09168451 NLM ISO Abbreviation: Biosci Biotechnol Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : Oxford : Oxford University Press Original Publication: Tokyo, Japan : Japan Society for Bioscience, Biotechnology, and Agrochemistry, c1992- |
| مواضيع طبية MeSH: | Lectins*/metabolism , Carbohydrates*/chemistry, Hexoses ; Agglutination ; Plant Lectins/chemistry |
| مستخلص: | We present real-time observations of a structurally variable process for cross-linking agglutination between multivalent lectins and glycoclusters using a small-angle forward static light scattering (F-SLS) technique. In this study, a cross-linking agglutination reaction was carried out using a tetravalent Neu5Acα2,6LacNAc-glycocluster and Sambucus sieboldiana agglutinin (SSA). The scattering intensity of time-resolved F-SLS increased with formation of the Neu5Acα2,6LacNAc-glycocluster-SSA cross-linked complex. Using this approach, fine sequential cross-linking agglutination between glycoclusters and lectins was observed in real-time. The rate of increase in the intensity of time-resolved F-SLS increased with the concentration of sialo-glycoclusters and SSA. Structural analysis based on the fractal dimension using time-resolved F-SLS patterns revealed that the density of the aggregates changed with progression of the cross-linking reaction until equilibrium was reached. This is the first report to evaluate the cross-linking agglutination reaction between glycoclusters and lectins and analysis of the subsequent structure of the obtained aggregates using time-resolved measurements of F-SLS. (© The Author(s) 2023. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.) |
| معلومات مُعتمدة: | 20K05855 JSPS |
| فهرسة مساهمة: | Keywords: agglutination; carbohydrate; glycocluster; lectin; small-angle forward static light scattering |
| المشرفين على المادة: | 0 (Lectins) 0 (Carbohydrates) 0 (Hexoses) 0 (Plant Lectins) |
| تواريخ الأحداث: | Date Created: 20230622 Date Completed: 20230824 Latest Revision: 20230824 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1093/bbb/zbad082 |
| PMID: | 37348468 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1347-6947 |
|---|---|
| DOI: | 10.1093/bbb/zbad082 |