دورية أكاديمية
Evaluation of MALDI-TOF MS, sequencing of D2 LSU rRNA and internal transcribed spacer regions (ITS) for the identification of filamentous fungi isolated from a pharmaceutical facility.
| العنوان: | Evaluation of MALDI-TOF MS, sequencing of D2 LSU rRNA and internal transcribed spacer regions (ITS) for the identification of filamentous fungi isolated from a pharmaceutical facility. |
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| المؤلفون: | Moreira FM; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil. Electronic address: marcelo.brandao@bio.fiocruz.br., Pereira PA; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Miranda RVDSL; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Reis CMFD; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Braga LMPDS; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., de Andrade JM; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., do Nascimento LG; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Mattoso JMV; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Forsythe SJ; Foodmicrobe.com, Adams Hill, Keyworth, Nottinghamshire NG12 5GY, United Kingdom., da Costa LV; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil., Brandão MLL; Microbiological Control Laboratory, Bio-manguinhos/Fiocruz, Brazil. |
| المصدر: | Journal of pharmaceutical and biomedical analysis [J Pharm Biomed Anal] 2023 Sep 20; Vol. 234, pp. 115531. Date of Electronic Publication: 2023 Jun 14. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Science Country of Publication: England NLM ID: 8309336 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-264X (Electronic) Linking ISSN: 07317085 NLM ISO Abbreviation: J Pharm Biomed Anal Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2006->: London : Elsevier Science Original Publication: Oxford ; New York : Pergamon Press, c1983- |
| مواضيع طبية MeSH: | Fungi*/genetics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization/methods ; Sequence Analysis, DNA ; Databases, Factual ; Pharmaceutical Preparations |
| مستخلص: | The identification of filamentous fungi through culture characterization may be hampered by phenotypic variability. Information obtained from the identification of microorganisms are important for investigation of sources of contamination of a product or process. The aim of this study was to identify filamentous fungal strains (n = 50) isolated from a pharmaceutical facility by using Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), as well as D2 domain of the large-subunit (LSU) ribosomal RNA gene and internal transcribed spacer regions (ITS) sequencing. MALDI-TOF MS system only identified five strains at the species level, while 45 were not identified. The analysis through GenBank allowed the identification of up to 19 strains at the species level, while MycoBank allowed the identification of up to nine strains at the species level. The databases identified up to 11 genera: Penicillium, Aspergillus, Cladosporium, Chaetomium, Coniochaeta, Curvularia, Diaporthe, Fusarium, Trichoderma, Rhizopus and Microdochium. MALDI-TOF MS showed an insufficient database to identify the species of fungi. DNA sequencing was the best methodology to identify to the genus level but was unable to differentiate between closely related species. Therefore further methods for the identification of filamentous fungi from pharmaceutical areas at species level need to be developed. Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (Copyright © 2023 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Fungi filamentous; Pharmaceutical facility; Protein analysis; Sequencing |
| المشرفين على المادة: | 0 (Pharmaceutical Preparations) |
| تواريخ الأحداث: | Date Created: 20230624 Date Completed: 20230828 Latest Revision: 20230828 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.jpba.2023.115531 |
| PMID: | 37354630 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-264X |
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| DOI: | 10.1016/j.jpba.2023.115531 |