دورية أكاديمية
A study of the effectiveness of a detergent-based California mastitis test (CMT), using Ethiopian and Nigerian domestic detergents, for the detection of high somatic cell counts in milk and their reliability compared to the commercial UK CMT.
| العنوان: | A study of the effectiveness of a detergent-based California mastitis test (CMT), using Ethiopian and Nigerian domestic detergents, for the detection of high somatic cell counts in milk and their reliability compared to the commercial UK CMT. |
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| المؤلفون: | Rust JD; Bristol Veterinary School, University of Bristol, Langford, Bristol, BS40 5DU, UK., Christian MJ; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Vance CJ; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Bolajoko MB; National Veterinary Research Institute, Vom, Nigeria., Wong JT; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Suarez-Martinez J; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Allan FK; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK., Peters AR; Centre for Supporting Evidence Based Interventions-Livestock, University of Edinburgh, Easter Bush, Midlothian, EH25 9RG, UK. |
| المصدر: | Gates open research [Gates Open Res] 2023 May 17; Vol. 5, pp. 146. Date of Electronic Publication: 2023 May 17 (Print Publication: 2021). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Bill and Melinda Gates Foundation Country of Publication: United States NLM ID: 101717821 Publication Model: eCollection Cited Medium: Internet ISSN: 2572-4754 (Electronic) Linking ISSN: 25724754 NLM ISO Abbreviation: Gates Open Res Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Seattle, WA : Bill and Melinda Gates Foundation, [2017]- |
| مستخلص: | Background: The California mastitis test (CMT) is a simple cow-side indicator of the somatic cell count (SCC) in milk, providing a useful tool in identifying cases of subclinical mastitis in cattle. Mastitis, and in particular subclinical mastitis, is a major concern in Ethiopia and Nigeria, yet detection is challenging due to cost and access to commercial CMT reagents. Methods: Commercially available domestic detergents from Ethiopia and Nigeria were compared (n = 3 for each country) with the UK commercial CMT reagent in their ability to detect high SCC (>400,000 cells/ml milk). Sensitivity and specificity of the CMT test were calculated for the different detergents and positive and negative predictive values were established. Results: The average sensitivities of the tests ranged from 28-75% for the Ethiopian detergents and 68-80% for the Nigerian detergents, compared to 76% for the UK domestic detergent. Test specificities were 84-98%, 93-97% and 96%, respectively. Conclusions: Overall, the detergents demonstrated higher specificity than sensitivity. Nigerian detergents performed better than the Ethiopian products, however, the study identified suitable domestic detergents from both Ethiopia and Nigeria, comparable to the UK commercial CMT reagent, and we recommend their use as alternative CMT reagents for livestock-keepers to aid in cost-effective diagnosis of mastitis. Competing Interests: No competing interests were disclosed. (Copyright: © 2023 Rust JD et al.) |
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| فهرسة مساهمة: | Keywords: California mastitis test; Mastitis; reagent; sensitivity; somatic cell count; specificity |
| تواريخ الأحداث: | Date Created: 20230626 Latest Revision: 20230701 |
| رمز التحديث: | 20240628 |
| مُعرف محوري في PubMed: | PMC10285044 |
| DOI: | 10.12688/gatesopenres.13369.2 |
| PMID: | 37362381 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2572-4754 |
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| DOI: | 10.12688/gatesopenres.13369.2 |