دورية أكاديمية
أعرض في EDS

A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.

التفاصيل البيبلوغرافية
العنوان: A novel cryopreservation and biobanking strategy to study lymphoid tissue stromal cells in human disease.
المؤلفون: Brandstadter JD; Division of Hematology/Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., De Martin A; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland., Lϋtge M; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland., Ferreira A; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA., Gaudette BT; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Stanossek Y; Department of Otorhinolaryngology, Head and Neck Surgery, Kantonsspital St. Gallen, St. Gallen, Switzerland., Wang S; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA., Gonzalez MV; Center for Cytokine Storm Treatment and Laboratory, Division of Translational Medicine and Human Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Camiolo E; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Wertheim G; Department of Pathology and Laboratory Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA., Austin B; Center for Cytokine Storm Treatment and Laboratory, Division of Translational Medicine and Human Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Allman D; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Bagg A; Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Lim MS; Department of Pathology and Laboratory Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, USA., Fajgenbaum DC; Center for Cytokine Storm Treatment and Laboratory, Division of Translational Medicine and Human Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA., Aster JC; Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA., Ludewig B; Institute of Immunobiology, Kantonsspital St. Gallen, St. Gallen, Switzerland., Maillard I; Division of Hematology/Oncology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
المصدر: European journal of immunology [Eur J Immunol] 2023 Sep; Vol. 53 (9), pp. e2250362. Date of Electronic Publication: 2023 Jun 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Wiley-VCH Country of Publication: Germany NLM ID: 1273201 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1521-4141 (Electronic) Linking ISSN: 00142980 NLM ISO Abbreviation: Eur J Immunol Subsets: MEDLINE
أسماء مطبوعة: Publication: <2005->: Weinheim : Wiley-VCH
Original Publication: Weinheim, Verlag Chemie GmbH.
مواضيع طبية MeSH: Biological Specimen Banks* , Cryopreservation*, Humans ; Lymphocytes ; Lymph Nodes/pathology ; Stromal Cells
مستخلص: Nonhematopoietic lymph node stromal cells (LNSCs) regulate lymphocyte trafficking, survival, and function for key roles in host defense, autoimmunity, alloimmunity, and lymphoproliferative disorders. However, the study of LNSCs in human diseases is complicated by a dependence on viable lymphoid tissues, which are most often excised prior to establishment of a specific diagnosis. Here, we demonstrate that cryopreservation can be used to bank lymphoid tissue for the study of LNSCs in human disease. Using human tonsils and lymph nodes (LN), lymphoid tissue fragments were cryopreserved for subsequent enzymatic digestion and recovery of viable nonhematopoietic cells. Flow cytometry and single-cell transcriptomics identified comparable proportions of LN stromal cell types in fresh and cryopreserved tissue. Moreover, cryopreservation had little effect on transcriptional profiles, which showed significant overlap between tonsils and LN. The presence and spatial distribution of transcriptionally defined cell types were confirmed by in situ analyses. Our broadly applicable approach promises to greatly enable research into the roles of LNSCs in human disease.
(© 2023 Wiley-VCH GmbH.)
التعليقات: Update of: bioRxiv. 2023 Feb 07;:. (PMID: 36798373)
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معلومات مُعتمدة: T32 HL007439 United States HL NHLBI NIH HHS; R01 AI091627 United States AI NIAID NIH HHS; T32 CA009140 United States CA NCI NIH HHS; P30 CA008748 United States CA NCI NIH HHS; R01 HL141408 United States HL NHLBI NIH HHS
فهرسة مساهمة: Keywords: Cryopreservation; LN; Lymphoid tissue fibroblasts; Stromal cells; Tonsil
تواريخ الأحداث: Date Created: 20230627 Date Completed: 20230911 Latest Revision: 20231122
رمز التحديث: 20231122
مُعرف محوري في PubMed: PMC10529925
DOI: 10.1002/eji.202250362
PMID: 37366295
قاعدة البيانات: MEDLINE
الوصف
تدمد:1521-4141
DOI:10.1002/eji.202250362